Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. Halla

Novel microbe-derived products are gaining increasing attention for their ability to modulate skin conditions. The use of microbial metabolites to improve skin health outcomes is of particular interest because growing evidence points to the importance of natural products without side effects on huma...

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Main Authors: Min Nyeong Ko, Chang-Gu Hyun
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Cosmetics
Subjects:
Online Access:https://www.mdpi.com/2079-9284/9/4/73
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author Min Nyeong Ko
Chang-Gu Hyun
author_facet Min Nyeong Ko
Chang-Gu Hyun
author_sort Min Nyeong Ko
collection DOAJ
description Novel microbe-derived products are gaining increasing attention for their ability to modulate skin conditions. The use of microbial metabolites to improve skin health outcomes is of particular interest because growing evidence points to the importance of natural products without side effects on human health. This study aimed to sequence the genome of <i>Viridibacillus</i> sp. JNUCC6 isolated from Baengnokdam, the summit crater of Mt. Halla. We further investigated the potential use of its extract as a cosmetic ingredient in controlling melanogenesis and inflammation. The genome of this strain was sequenced using both Illumina Novaseq 6000 and third-generation sequencing technology (PacBio RSII) to obtain trustworthy assembly and annotation. Different concentrations of the <i>Viridibacillus</i> sp. JNUCC6 extract were tested for its anti-melanogenic and anti-inflammatory effects in α-melanocyte-stimulating hormone (α-MSH)-induced B16F10 melanoma and lipopolysaccharide (LPS)-activated RAW 264.7 cells, respectively. The whole genome sequence of the strain contained 4,526,142 bp with 35.61% GC content, one contig, and 4364 protein-coding sequences. Furthermore, antiSMASH analysis of the whole genome revealed three putative biosynthetic gene clusters that are responsible for the production of various secondary metabolites. Our study found that the <i>Viridibacillus</i> sp. JNUCC6 extract inhibited the α-MSH-induced melanin production and tyrosinase activity in B16F10 melanoma cells. In addition, it decreased the LPS-induced nitric oxide (NO) production caused by LPS stimulation in a concentration-dependent manner. Therefore, <i>Viridibacillus</i> sp. JNUCC6 has potential applications as an ingredient in skin-whitening and anti-inflammatory products and can be used in the cosmetic and medical industries.
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spelling doaj.art-471bfafb74804dbaa61d1c53792164962023-11-30T21:09:56ZengMDPI AGCosmetics2079-92842022-07-01947310.3390/cosmetics9040073Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. HallaMin Nyeong Ko0Chang-Gu Hyun1Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, KoreaJeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, KoreaNovel microbe-derived products are gaining increasing attention for their ability to modulate skin conditions. The use of microbial metabolites to improve skin health outcomes is of particular interest because growing evidence points to the importance of natural products without side effects on human health. This study aimed to sequence the genome of <i>Viridibacillus</i> sp. JNUCC6 isolated from Baengnokdam, the summit crater of Mt. Halla. We further investigated the potential use of its extract as a cosmetic ingredient in controlling melanogenesis and inflammation. The genome of this strain was sequenced using both Illumina Novaseq 6000 and third-generation sequencing technology (PacBio RSII) to obtain trustworthy assembly and annotation. Different concentrations of the <i>Viridibacillus</i> sp. JNUCC6 extract were tested for its anti-melanogenic and anti-inflammatory effects in α-melanocyte-stimulating hormone (α-MSH)-induced B16F10 melanoma and lipopolysaccharide (LPS)-activated RAW 264.7 cells, respectively. The whole genome sequence of the strain contained 4,526,142 bp with 35.61% GC content, one contig, and 4364 protein-coding sequences. Furthermore, antiSMASH analysis of the whole genome revealed three putative biosynthetic gene clusters that are responsible for the production of various secondary metabolites. Our study found that the <i>Viridibacillus</i> sp. JNUCC6 extract inhibited the α-MSH-induced melanin production and tyrosinase activity in B16F10 melanoma cells. In addition, it decreased the LPS-induced nitric oxide (NO) production caused by LPS stimulation in a concentration-dependent manner. Therefore, <i>Viridibacillus</i> sp. JNUCC6 has potential applications as an ingredient in skin-whitening and anti-inflammatory products and can be used in the cosmetic and medical industries.https://www.mdpi.com/2079-9284/9/4/73cosmeticsdraft genome sequenceinflammationmelanogenesis<i>Viridibacillus</i>
spellingShingle Min Nyeong Ko
Chang-Gu Hyun
Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. Halla
Cosmetics
cosmetics
draft genome sequence
inflammation
melanogenesis
<i>Viridibacillus</i>
title Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. Halla
title_full Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. Halla
title_fullStr Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. Halla
title_full_unstemmed Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. Halla
title_short Complete Genome Sequence and Cosmetic Potential of <i>Viridibacillus</i> sp. JNUCC6 Isolated from Baengnokdam, the Summit Crater of Mt. Halla
title_sort complete genome sequence and cosmetic potential of i viridibacillus i sp jnucc6 isolated from baengnokdam the summit crater of mt halla
topic cosmetics
draft genome sequence
inflammation
melanogenesis
<i>Viridibacillus</i>
url https://www.mdpi.com/2079-9284/9/4/73
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