An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells
Abstract Photodynamic therapy is becoming increasingly popular for combat of bacteria. In the clinical photodynamic combat of bacteria, one critical issue is to avoid the potential damage to the host since the reactive oxygen species produced by photosensitizers are also harmful to mammalian cells....
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-11-01
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| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10539 |
| _version_ | 1797556842947149824 |
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| author | Fei Wang Yupeng Shi Po‐Yu Ho Engui Zhao Chuen Kam Qiang Zhang Xin Zhao Yue Pan Sijie Chen |
| author_facet | Fei Wang Yupeng Shi Po‐Yu Ho Engui Zhao Chuen Kam Qiang Zhang Xin Zhao Yue Pan Sijie Chen |
| author_sort | Fei Wang |
| collection | DOAJ |
| description | Abstract Photodynamic therapy is becoming increasingly popular for combat of bacteria. In the clinical photodynamic combat of bacteria, one critical issue is to avoid the potential damage to the host since the reactive oxygen species produced by photosensitizers are also harmful to mammalian cells. In this work, we report an aggregation‐induced‐emission‐active bacterial inhibitor and photosensitizer, OEO‐TPE‐MEM (OTM), for the imaging, killing, and light‐enhanced inactivation of bacteria. OTM could efficiently bind to and kill Gram‐positive bacteria, while its affinity to Gram‐negative bacteria is lower, and a higher OTM concentration is required for killing Gram‐negative bacteria. OTM is also an efficient photosensitizer and could efficiently sensitize the production of reactive oxygen species, which enhances its killing effect on both Gram‐positive and Gram‐negative bacteria. More interestingly, OTM is very biocompatible with normal mammalian cells both in the dark and under light irradiation. OTM in mice models with bacteria‐infected wounds could promote the healing of infected wounds without affecting their organs and blood parameters, which makes it an excellent candidate for clinical applications. |
| first_indexed | 2024-03-10T17:08:39Z |
| format | Article |
| id | doaj.art-471f5156f2f441c1bc03cb0254361a53 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-03-10T17:08:39Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-471f5156f2f441c1bc03cb0254361a532023-11-20T10:44:13ZengWileyBioengineering & Translational Medicine2380-67612023-11-0186n/an/a10.1002/btm2.10539An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cellsFei Wang0Yupeng Shi1Po‐Yu Ho2Engui Zhao3Chuen Kam4Qiang Zhang5Xin Zhao6Yue Pan7Sijie Chen8School of Science Harbin Institute of Technology, Shenzhen, HIT Campus of University Town Shenzhen ChinaMing Wai Lau Centre for Reparative Medicine Karolinska Institutet Hong Kong ChinaMing Wai Lau Centre for Reparative Medicine Karolinska Institutet Hong Kong ChinaSchool of Science Harbin Institute of Technology, Shenzhen, HIT Campus of University Town Shenzhen ChinaMing Wai Lau Centre for Reparative Medicine Karolinska Institutet Hong Kong ChinaDepartment of Biomedical Engineering The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Biomedical Engineering The Hong Kong Polytechnic University Hong Kong ChinaGuangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong‐Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat‐Sen Memorial Hospital Sun Yat‐Sen University Guangzhou ChinaMing Wai Lau Centre for Reparative Medicine Karolinska Institutet Hong Kong ChinaAbstract Photodynamic therapy is becoming increasingly popular for combat of bacteria. In the clinical photodynamic combat of bacteria, one critical issue is to avoid the potential damage to the host since the reactive oxygen species produced by photosensitizers are also harmful to mammalian cells. In this work, we report an aggregation‐induced‐emission‐active bacterial inhibitor and photosensitizer, OEO‐TPE‐MEM (OTM), for the imaging, killing, and light‐enhanced inactivation of bacteria. OTM could efficiently bind to and kill Gram‐positive bacteria, while its affinity to Gram‐negative bacteria is lower, and a higher OTM concentration is required for killing Gram‐negative bacteria. OTM is also an efficient photosensitizer and could efficiently sensitize the production of reactive oxygen species, which enhances its killing effect on both Gram‐positive and Gram‐negative bacteria. More interestingly, OTM is very biocompatible with normal mammalian cells both in the dark and under light irradiation. OTM in mice models with bacteria‐infected wounds could promote the healing of infected wounds without affecting their organs and blood parameters, which makes it an excellent candidate for clinical applications.https://doi.org/10.1002/btm2.10539aggregation‐induced emissionbacterial inhibitorphotodynamic therapyphotosensitizerwound healing |
| spellingShingle | Fei Wang Yupeng Shi Po‐Yu Ho Engui Zhao Chuen Kam Qiang Zhang Xin Zhao Yue Pan Sijie Chen An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells Bioengineering & Translational Medicine aggregation‐induced emission bacterial inhibitor photodynamic therapy photosensitizer wound healing |
| title | An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells |
| title_full | An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells |
| title_fullStr | An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells |
| title_full_unstemmed | An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells |
| title_short | An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells |
| title_sort | aie active bacterial inhibitor and photosensitizer for selective imaging killing and photodynamic inactivation of bacteria over mammalian cells |
| topic | aggregation‐induced emission bacterial inhibitor photodynamic therapy photosensitizer wound healing |
| url | https://doi.org/10.1002/btm2.10539 |
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