Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative
Abstract Organoids hold inestimable therapeutic potential in regenerative medicine and are increasingly serving as an in vitro research platform. Still, their expanding applications are critically restricted by the canonical culture matrix and system. Synthesis of a suitable bioink of bioactivity, b...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10327 |
| _version_ | 1797270834136481792 |
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| author | Zi‐Yan Xu Jin‐Jian Huang Ye Liu Can‐Wen Chen Gui‐Wen Qu Ge‐Fei Wang Yun Zhao Xiu‐Wen Wu Jian‐An Ren |
| author_facet | Zi‐Yan Xu Jin‐Jian Huang Ye Liu Can‐Wen Chen Gui‐Wen Qu Ge‐Fei Wang Yun Zhao Xiu‐Wen Wu Jian‐An Ren |
| author_sort | Zi‐Yan Xu |
| collection | DOAJ |
| description | Abstract Organoids hold inestimable therapeutic potential in regenerative medicine and are increasingly serving as an in vitro research platform. Still, their expanding applications are critically restricted by the canonical culture matrix and system. Synthesis of a suitable bioink of bioactivity, biosecurity, tunable stiffness, and printability to replace conventional matrices and fabricate customized culture systems remains challenging. Here, we envisaged a novel bioink formulation based on decellularized extracellular matrix (dECM) from porcine small intestinal submucosa for organoids bioprinting, which provides intestinal stem cells (ISCs) with niche‐specific ECM content and biomimetic microstructure. Intestinal organoids cultured in the fabricated bioink exhibited robust generation as well as a distinct differentiation pattern and transcriptomic signature. This bioink established a new co‐culture system able to study interaction between epithelial homeostasis and submucosal cells and promote organoids maturation after transplantation into the mesentery of immune‐deficient NODSCID‐gamma (NSG) mice. In summary, the development of such photo‐responsive bioink has the potential to replace tumor‐derived Matrigel and facilitate the application of organoids in translational medicine and disease modeling. |
| first_indexed | 2024-04-25T02:10:34Z |
| format | Article |
| id | doaj.art-47208b1206eb4925a42facbd2f15f1fb |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-04-25T02:10:34Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-47208b1206eb4925a42facbd2f15f1fb2024-03-07T12:20:54ZengWileyBioengineering & Translational Medicine2380-67612023-01-0181n/an/a10.1002/btm2.10327Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternativeZi‐Yan Xu0Jin‐Jian Huang1Ye Liu2Can‐Wen Chen3Gui‐Wen Qu4Ge‐Fei Wang5Yun Zhao6Xiu‐Wen Wu7Jian‐An Ren8Research Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing University Nanjing Jiangsu Province ChinaSchool of Medicine, Southeast University Nanjing Jiangsu Province ChinaSchool of Medicine, Southeast University Nanjing Jiangsu Province ChinaResearch Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing University Nanjing Jiangsu Province ChinaSchool of Medicine, Southeast University Nanjing Jiangsu Province ChinaResearch Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing University Nanjing Jiangsu Province ChinaDepartment of General Surgery, BenQ Medical Center Nanjing Jiangsu Province ChinaResearch Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing University Nanjing Jiangsu Province ChinaResearch Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing University Nanjing Jiangsu Province ChinaAbstract Organoids hold inestimable therapeutic potential in regenerative medicine and are increasingly serving as an in vitro research platform. Still, their expanding applications are critically restricted by the canonical culture matrix and system. Synthesis of a suitable bioink of bioactivity, biosecurity, tunable stiffness, and printability to replace conventional matrices and fabricate customized culture systems remains challenging. Here, we envisaged a novel bioink formulation based on decellularized extracellular matrix (dECM) from porcine small intestinal submucosa for organoids bioprinting, which provides intestinal stem cells (ISCs) with niche‐specific ECM content and biomimetic microstructure. Intestinal organoids cultured in the fabricated bioink exhibited robust generation as well as a distinct differentiation pattern and transcriptomic signature. This bioink established a new co‐culture system able to study interaction between epithelial homeostasis and submucosal cells and promote organoids maturation after transplantation into the mesentery of immune‐deficient NODSCID‐gamma (NSG) mice. In summary, the development of such photo‐responsive bioink has the potential to replace tumor‐derived Matrigel and facilitate the application of organoids in translational medicine and disease modeling.https://doi.org/10.1002/btm2.10327bioprintingdECM‐inkintestinal organoidsphoto‐crosslinkingstemnesstransplantation |
| spellingShingle | Zi‐Yan Xu Jin‐Jian Huang Ye Liu Can‐Wen Chen Gui‐Wen Qu Ge‐Fei Wang Yun Zhao Xiu‐Wen Wu Jian‐An Ren Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative Bioengineering & Translational Medicine bioprinting dECM‐ink intestinal organoids photo‐crosslinking stemness transplantation |
| title | Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative |
| title_full | Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative |
| title_fullStr | Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative |
| title_full_unstemmed | Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative |
| title_short | Extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe Matrigel alternative |
| title_sort | extracellular matrix bioink boosts stemness and facilitates transplantation of intestinal organoids as a biosafe matrigel alternative |
| topic | bioprinting dECM‐ink intestinal organoids photo‐crosslinking stemness transplantation |
| url | https://doi.org/10.1002/btm2.10327 |
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