Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247
The present work had as objective to achieve the formation of nodular calli in Phaseolus vulgaris L. cv. CIAP 7247 using several growth regulators in the culture medium. Cotyledons of mature seeds without coats were used as explants. The effect of different concentrations of TDZ, 2,4-D and AIA was e...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2006-04-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/406 |
| _version_ | 1818118513976410112 |
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| author | Lourdes R. García Jorge Pérez Pérez Damaris Torres Yenny Padrón |
| author_facet | Lourdes R. García Jorge Pérez Pérez Damaris Torres Yenny Padrón |
| author_sort | Lourdes R. García |
| collection | DOAJ |
| description | The present work had as objective to achieve the formation of nodular calli in Phaseolus vulgaris L. cv. CIAP 7247 using several growth regulators in the culture medium. Cotyledons of mature seeds without coats were used as explants. The effect of different concentrations of TDZ, 2,4-D and AIA was evauated for the callus formation. In the treatment with different concentrations of TDZ, the explants showed green-yellow coloration during the first two weeks of culture. When the callus growth was evaluated the best values were obtained when 0.2 mg.l-1 TDZ was used. When the effect of 2,4-D was evaluated in the culture medium were not found significant differences in the explants number with callus formation for the studied concentrations. These were not compact, have watery consistency and yellow-whitish coloration without presenting visually characteristic of nodular calli. Nevertheless, those formed in the culture medium with AIA were less voluminous, but they presented better characteristics in their consistency. It was demonstrated that the combination of TDZ-AIA was the best for the formation of nodular calli in Phaseolus vulgaris cv. CIAP 7247.
Key words: beans, culture medium, nodular calli, tissue culture |
| first_indexed | 2024-12-11T04:55:30Z |
| format | Article |
| id | doaj.art-4720eaa0f150489bbbb5dbc15b5a1467 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-11T04:55:30Z |
| publishDate | 2006-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-4720eaa0f150489bbbb5dbc15b5a14672022-12-22T01:20:16ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472006-04-0162376Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247Lourdes R. GarcíaJorge Pérez PérezDamaris TorresYenny PadrónThe present work had as objective to achieve the formation of nodular calli in Phaseolus vulgaris L. cv. CIAP 7247 using several growth regulators in the culture medium. Cotyledons of mature seeds without coats were used as explants. The effect of different concentrations of TDZ, 2,4-D and AIA was evauated for the callus formation. In the treatment with different concentrations of TDZ, the explants showed green-yellow coloration during the first two weeks of culture. When the callus growth was evaluated the best values were obtained when 0.2 mg.l-1 TDZ was used. When the effect of 2,4-D was evaluated in the culture medium were not found significant differences in the explants number with callus formation for the studied concentrations. These were not compact, have watery consistency and yellow-whitish coloration without presenting visually characteristic of nodular calli. Nevertheless, those formed in the culture medium with AIA were less voluminous, but they presented better characteristics in their consistency. It was demonstrated that the combination of TDZ-AIA was the best for the formation of nodular calli in Phaseolus vulgaris cv. CIAP 7247. Key words: beans, culture medium, nodular calli, tissue culturehttps://revista.ibp.co.cu/index.php/BV/article/view/406 |
| spellingShingle | Lourdes R. García Jorge Pérez Pérez Damaris Torres Yenny Padrón Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247 Biotecnología Vegetal |
| title | Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247 |
| title_full | Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247 |
| title_fullStr | Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247 |
| title_full_unstemmed | Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247 |
| title_short | Influence of growth regulators in the callus formation of Phaseolus vulgaris L cv. CIAP 7247 |
| title_sort | influence of growth regulators in the callus formation of phaseolus vulgaris l cv ciap 7247 |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/406 |
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