RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma.
Receptor activity modifying protein 1 (RAMP1) facilitates the localization of the calcitonin-like receptor (CLR) to the plasma membrane, but its role in osteosarcoma (OS) remains unclear. We evaluated the RAMP1 expression and prognostic value across different cancers, studying tumor immune infiltrat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292452&type=printable |
| _version_ | 1797635254223110144 |
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| author | Long Xie Weiwei Xiao Hangqi Fang Guoqiang Liu |
| author_facet | Long Xie Weiwei Xiao Hangqi Fang Guoqiang Liu |
| author_sort | Long Xie |
| collection | DOAJ |
| description | Receptor activity modifying protein 1 (RAMP1) facilitates the localization of the calcitonin-like receptor (CLR) to the plasma membrane, but its role in osteosarcoma (OS) remains unclear. We evaluated the RAMP1 expression and prognostic value across different cancers, studying tumor immune infiltration. The prognostic value was analyzed using the GSE39058 and TARGET datasets. Differential gene expression was evaluated. a protein-protein interaction network was constructed, and gene set enrichment analysis was performed. The function of RAMP1 in the tumor microenvironment was analyzed, and its expression in OS cell lines was validated using quantitative real-time PCR. High RAMP1 expression correlated with poor prognosis relative to low RAMP1 expression (p < 0.05). Low RAMP1 expression correlated with an abundance of CD4+ memory-activated T cells. whereas a high expression level correlated with a high proportion of gamma-delta T cells (γδ T cells). Differentially expressed genes from TARGET was enriched in olfactory transduction pathways (normalized enrichment scores [NES] = 1.6998, p < 0.0001). RAMP1 expression negatively correlated with CD44 expression but positively correlated with TNFSF9 expression. The RAMP1 gene is substantially expressed in OS cells compared to the normal osteoblast cell line hFOB1.19. Thus, RAMP1 may be a prognostic biomarker and potential therapeutic target in OS. |
| first_indexed | 2024-03-11T12:18:36Z |
| format | Article |
| id | doaj.art-472416bc7fd14ca2a9a1109549a13a52 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T12:18:36Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-472416bc7fd14ca2a9a1109549a13a522023-11-07T05:34:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-011810e029245210.1371/journal.pone.0292452RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma.Long XieWeiwei XiaoHangqi FangGuoqiang LiuReceptor activity modifying protein 1 (RAMP1) facilitates the localization of the calcitonin-like receptor (CLR) to the plasma membrane, but its role in osteosarcoma (OS) remains unclear. We evaluated the RAMP1 expression and prognostic value across different cancers, studying tumor immune infiltration. The prognostic value was analyzed using the GSE39058 and TARGET datasets. Differential gene expression was evaluated. a protein-protein interaction network was constructed, and gene set enrichment analysis was performed. The function of RAMP1 in the tumor microenvironment was analyzed, and its expression in OS cell lines was validated using quantitative real-time PCR. High RAMP1 expression correlated with poor prognosis relative to low RAMP1 expression (p < 0.05). Low RAMP1 expression correlated with an abundance of CD4+ memory-activated T cells. whereas a high expression level correlated with a high proportion of gamma-delta T cells (γδ T cells). Differentially expressed genes from TARGET was enriched in olfactory transduction pathways (normalized enrichment scores [NES] = 1.6998, p < 0.0001). RAMP1 expression negatively correlated with CD44 expression but positively correlated with TNFSF9 expression. The RAMP1 gene is substantially expressed in OS cells compared to the normal osteoblast cell line hFOB1.19. Thus, RAMP1 may be a prognostic biomarker and potential therapeutic target in OS.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292452&type=printable |
| spellingShingle | Long Xie Weiwei Xiao Hangqi Fang Guoqiang Liu RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma. PLoS ONE |
| title | RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma. |
| title_full | RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma. |
| title_fullStr | RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma. |
| title_full_unstemmed | RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma. |
| title_short | RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma. |
| title_sort | ramp1 as a novel prognostic biomarker in pan cancer and osteosarcoma |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292452&type=printable |
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