Obesity and follicular fluid oxidative stress: Relationship to ICSI outcome
Objective: To study the influence of female obesity on follicular fluid oxidative stress and to correlate it to intracytoplasmic sperm injection (ICSI) outcome. Study design: Seventy-four normal females below the age of 40 undergoing ICSI for reason of male factor infertility were enrolled in the st...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2014-06-01
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| Series: | Middle East Fertility Society Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569013000927 |
| _version_ | 1819075623399718912 |
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| author | Eman Kamal Shaeer Soliman Adel Soliman Abdelwahab Abo-bakr El-Ghareeb |
| author_facet | Eman Kamal Shaeer Soliman Adel Soliman Abdelwahab Abo-bakr El-Ghareeb |
| author_sort | Eman Kamal Shaeer |
| collection | DOAJ |
| description | Objective: To study the influence of female obesity on follicular fluid oxidative stress and to correlate it to intracytoplasmic sperm injection (ICSI) outcome.
Study design: Seventy-four normal females below the age of 40 undergoing ICSI for reason of male factor infertility were enrolled in the study. They were divided into 2 groups according to body mass index (BMI); Group I (non-obese) (n = 24, BMI < 25 Kg/m2) and Group II (obese) (n = 50, BMI ⩾ 25 Kg/m2). Oxidative stress markers (MDA (malonaldehyde), NO2/NO3 (Nitrite/Nitrate) ratio, GSH (reduced glutathione) and GSH/GSSG (reduced glutathione/oxidized glutathione) ratio) were measured by high performance liquid chromatography (HPLC).
Results: Obese women had significantly higher mean follicular fluid MDA (P = 0.006) as well as NO2/NO3 ratio (P = 0.004). BMI strongly correlated to follicular fluid MDA (P = < 0.01). MDA showed strong positive correlation to NO2/NO3 ratio (P = 0.02). GSH and GSH/GSSG ratio showed a non-significant difference between the two groups (P = 0.14 and 0.67, respectively). Clinical pregnancy rate was significantly higher in the non-obese group (87%) compared to the obese (43%) (P = <0.01, OR:13; 95% CI 3.54–52). With binary logistic regression, MDA was found to be a good predictor of the occurrence of pregnancy (P = <0.01). No significant differences were detected as regards parameters including estradiol on day of hCG, gonadotropin dose, number of retrieved oocytes, metaphase II(MII) and fertilized oocytes.
Conclusion: Obesity aggravates follicular fluid oxidative stress with a negative impact on pregnancy outcome of ICSI cycles. |
| first_indexed | 2024-12-21T18:28:21Z |
| format | Article |
| id | doaj.art-472fc1bc7ea740fb8e8a05365ff03696 |
| institution | Directory Open Access Journal |
| issn | 1110-5690 |
| language | English |
| last_indexed | 2024-12-21T18:28:21Z |
| publishDate | 2014-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj.art-472fc1bc7ea740fb8e8a05365ff036962022-12-21T18:54:20ZengSpringerOpenMiddle East Fertility Society Journal1110-56902014-06-0119213914310.1016/j.mefs.2013.07.002Obesity and follicular fluid oxidative stress: Relationship to ICSI outcomeEman Kamal Shaeer0Soliman Adel Soliman1Abdelwahab Abo-bakr El-Ghareeb2Gynecology and Obstetrics, Cairo University, EgyptFaculty of Science, Cairo University, EgyptDepartment of Embryology, Faculty of Science, Cairo University, EgyptObjective: To study the influence of female obesity on follicular fluid oxidative stress and to correlate it to intracytoplasmic sperm injection (ICSI) outcome. Study design: Seventy-four normal females below the age of 40 undergoing ICSI for reason of male factor infertility were enrolled in the study. They were divided into 2 groups according to body mass index (BMI); Group I (non-obese) (n = 24, BMI < 25 Kg/m2) and Group II (obese) (n = 50, BMI ⩾ 25 Kg/m2). Oxidative stress markers (MDA (malonaldehyde), NO2/NO3 (Nitrite/Nitrate) ratio, GSH (reduced glutathione) and GSH/GSSG (reduced glutathione/oxidized glutathione) ratio) were measured by high performance liquid chromatography (HPLC). Results: Obese women had significantly higher mean follicular fluid MDA (P = 0.006) as well as NO2/NO3 ratio (P = 0.004). BMI strongly correlated to follicular fluid MDA (P = < 0.01). MDA showed strong positive correlation to NO2/NO3 ratio (P = 0.02). GSH and GSH/GSSG ratio showed a non-significant difference between the two groups (P = 0.14 and 0.67, respectively). Clinical pregnancy rate was significantly higher in the non-obese group (87%) compared to the obese (43%) (P = <0.01, OR:13; 95% CI 3.54–52). With binary logistic regression, MDA was found to be a good predictor of the occurrence of pregnancy (P = <0.01). No significant differences were detected as regards parameters including estradiol on day of hCG, gonadotropin dose, number of retrieved oocytes, metaphase II(MII) and fertilized oocytes. Conclusion: Obesity aggravates follicular fluid oxidative stress with a negative impact on pregnancy outcome of ICSI cycles.http://www.sciencedirect.com/science/article/pii/S1110569013000927ObesityOxidative stressAntioxidantsICSIPregnancy rate |
| spellingShingle | Eman Kamal Shaeer Soliman Adel Soliman Abdelwahab Abo-bakr El-Ghareeb Obesity and follicular fluid oxidative stress: Relationship to ICSI outcome Middle East Fertility Society Journal Obesity Oxidative stress Antioxidants ICSI Pregnancy rate |
| title | Obesity and follicular fluid oxidative stress: Relationship to ICSI outcome |
| title_full | Obesity and follicular fluid oxidative stress: Relationship to ICSI outcome |
| title_fullStr | Obesity and follicular fluid oxidative stress: Relationship to ICSI outcome |
| title_full_unstemmed | Obesity and follicular fluid oxidative stress: Relationship to ICSI outcome |
| title_short | Obesity and follicular fluid oxidative stress: Relationship to ICSI outcome |
| title_sort | obesity and follicular fluid oxidative stress relationship to icsi outcome |
| topic | Obesity Oxidative stress Antioxidants ICSI Pregnancy rate |
| url | http://www.sciencedirect.com/science/article/pii/S1110569013000927 |
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