Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning
Early microbial colonization in the gut impacts animal performance and lifelong health. However, research on gut microbial colonization and development in young ruminants, especially after weaning, is currently limited. In this study, next-generation sequencing technology was performed to investigat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/8/1111 |
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| author | Yimin Zhuang Jianmin Chai Kai Cui Yanliang Bi Qiyu Diao Wenqin Huang Hunter Usdrowski Naifeng Zhang |
| author_facet | Yimin Zhuang Jianmin Chai Kai Cui Yanliang Bi Qiyu Diao Wenqin Huang Hunter Usdrowski Naifeng Zhang |
| author_sort | Yimin Zhuang |
| collection | DOAJ |
| description | Early microbial colonization in the gut impacts animal performance and lifelong health. However, research on gut microbial colonization and development in young ruminants, especially after weaning, is currently limited. In this study, next-generation sequencing technology was performed to investigate the temporal dynamic changes of the microbial community in the jejunum and colon of goats at 1, 7, 14, 28, 42, 56, 70, and 84 days (d) of age. As age increased, significant increases in microbial diversity, including the number of Observed OTUs and the Shannon Index, were observed in both the jejunum and colon. Regarding beta diversity, significant shifts in community membership and structure from d1 to d84 were observed based on both Bray–Curtis and Jaccard distances. With increasing age, dominant genera in the jejunum shifted from <i>Lactobacillus</i> to unclassified <i>Ruminococcaceae</i>, unclassified <i>Lachnospiraceae</i> and unclassified <i>Clostridiales</i> through starter supplementation, whereas colonic dominant genera changed from <i>Lactobacillus</i> and <i>Butyricicoccus</i>, within d1–d28, to unclassified <i>Ruminococcaceae</i>, unclassified <i>Clostridiales</i> and <i>Campylobacter</i> after solid diet supplementation. The linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed bacterial features that are stage-specific in the jejunum and colon, respectively. In the jejunum and colon, a significantly distinct structure and membership of the microbiota was observed across all ages. The growth stage-associated microbiota in each gut compartment was also identified as a marker for biogeography. Our data indicate the temporal and spatial differences of the gut microbiota in goats are important for their performance and health. Early microbial colonization can influence microbial composition in later life (e.g., post-weaning phase). This study provides insights that the temporal dynamics of gut microbiota development from newborn to post-weaning can aid in developing feeding strategies to improve goat health and production. |
| first_indexed | 2024-03-10T18:14:51Z |
| format | Article |
| id | doaj.art-472fe0c208fc47e5980991d31ddd8b45 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T18:14:51Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-472fe0c208fc47e5980991d31ddd8b452023-11-20T07:49:13ZengMDPI AGMicroorganisms2076-26072020-07-0188111110.3390/microorganisms8081111Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to PostweaningYimin Zhuang0Jianmin Chai1Kai Cui2Yanliang Bi3Qiyu Diao4Wenqin Huang5Hunter Usdrowski6Naifeng Zhang7Feed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, ChinaDepartment of Animal Science, Division of Agriculture, University of Arkansas, Fayetteville, AR 72701, USAFeed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, ChinaEarly microbial colonization in the gut impacts animal performance and lifelong health. However, research on gut microbial colonization and development in young ruminants, especially after weaning, is currently limited. In this study, next-generation sequencing technology was performed to investigate the temporal dynamic changes of the microbial community in the jejunum and colon of goats at 1, 7, 14, 28, 42, 56, 70, and 84 days (d) of age. As age increased, significant increases in microbial diversity, including the number of Observed OTUs and the Shannon Index, were observed in both the jejunum and colon. Regarding beta diversity, significant shifts in community membership and structure from d1 to d84 were observed based on both Bray–Curtis and Jaccard distances. With increasing age, dominant genera in the jejunum shifted from <i>Lactobacillus</i> to unclassified <i>Ruminococcaceae</i>, unclassified <i>Lachnospiraceae</i> and unclassified <i>Clostridiales</i> through starter supplementation, whereas colonic dominant genera changed from <i>Lactobacillus</i> and <i>Butyricicoccus</i>, within d1–d28, to unclassified <i>Ruminococcaceae</i>, unclassified <i>Clostridiales</i> and <i>Campylobacter</i> after solid diet supplementation. The linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed bacterial features that are stage-specific in the jejunum and colon, respectively. In the jejunum and colon, a significantly distinct structure and membership of the microbiota was observed across all ages. The growth stage-associated microbiota in each gut compartment was also identified as a marker for biogeography. Our data indicate the temporal and spatial differences of the gut microbiota in goats are important for their performance and health. Early microbial colonization can influence microbial composition in later life (e.g., post-weaning phase). This study provides insights that the temporal dynamics of gut microbiota development from newborn to post-weaning can aid in developing feeding strategies to improve goat health and production.https://www.mdpi.com/2076-2607/8/8/1111gut microbiotagoatruminantsagesbiogeographytemporal dynamics |
| spellingShingle | Yimin Zhuang Jianmin Chai Kai Cui Yanliang Bi Qiyu Diao Wenqin Huang Hunter Usdrowski Naifeng Zhang Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning Microorganisms gut microbiota goat ruminants ages biogeography temporal dynamics |
| title | Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning |
| title_full | Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning |
| title_fullStr | Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning |
| title_full_unstemmed | Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning |
| title_short | Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning |
| title_sort | longitudinal investigation of the gut microbiota in goat kids from birth to postweaning |
| topic | gut microbiota goat ruminants ages biogeography temporal dynamics |
| url | https://www.mdpi.com/2076-2607/8/8/1111 |
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