The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway
ABSTRACT Purpose: To observe the mechanism of prepared Radix Rehmanniainon combined with Radix Astragali in treating osteoporosis. Methods: Osteoporosis rat model was established by bilateral ovariectomy combined with low-calcium diet feeding. Bone mineral density was measured by bone densitometer...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2023-01-01
|
| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001100200&tlng=en |
| _version_ | 1797871137164623872 |
|---|---|
| author | Wen-qian Kang Pei-feng Wei Li Ou Min Li Chun-yu Liu |
| author_facet | Wen-qian Kang Pei-feng Wei Li Ou Min Li Chun-yu Liu |
| author_sort | Wen-qian Kang |
| collection | DOAJ |
| description | ABSTRACT Purpose: To observe the mechanism of prepared Radix Rehmanniainon combined with Radix Astragali in treating osteoporosis. Methods: Osteoporosis rat model was established by bilateral ovariectomy combined with low-calcium diet feeding. Bone mineral density was measured by bone densitometer. Bone metabolism markers in serum were detected by enzyme linked immunosorbent assay (ELISA), bone tissue structure was observed by hematoxylin-eosin staining, and the effect of prepared Radix Rehmanniainon combined with Radix Astragali on PI3K-AKT signaling pathway was investigated by immunohistochemistry and reverse transcription polymerase chain reaction. Results: Compared with the model group, the bone tissue structure and imbalance of bone metabolism were improved, and the bone mineral density was significantly increased in the prepared Radix Rehmanniainon combined with Radix Astragali groups. After intervention with prepared Radix Rehmanniainon combined with Radix Astragali, the positive expression of PIK3CA and Akt1 in rat bone tissue was enhanced, and the expression levels of Akt1 mRNA were significantly increased. Conclusions: Prepared Radix Rehmanniainon combined with Radix Astragali may treat osteoporosis by activating PI3K/AKT pathway. |
| first_indexed | 2024-04-10T00:39:26Z |
| format | Article |
| id | doaj.art-472fe7ea20ff47d3be2793cae71243d9 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-10T00:39:26Z |
| publishDate | 2023-01-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-472fe7ea20ff47d3be2793cae71243d92023-03-14T07:32:52ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742023-01-01371110.1590/acb371101The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathwayWen-qian Kanghttps://orcid.org/0000-0003-2353-5254Pei-feng Weihttps://orcid.org/0000-0002-8652-5312Li Ouhttps://orcid.org/0000-0001-8767-1322Min Lihttps://orcid.org/0000-0003-0399-3696Chun-yu Liuhttps://orcid.org/0000-0001-6440-8871ABSTRACT Purpose: To observe the mechanism of prepared Radix Rehmanniainon combined with Radix Astragali in treating osteoporosis. Methods: Osteoporosis rat model was established by bilateral ovariectomy combined with low-calcium diet feeding. Bone mineral density was measured by bone densitometer. Bone metabolism markers in serum were detected by enzyme linked immunosorbent assay (ELISA), bone tissue structure was observed by hematoxylin-eosin staining, and the effect of prepared Radix Rehmanniainon combined with Radix Astragali on PI3K-AKT signaling pathway was investigated by immunohistochemistry and reverse transcription polymerase chain reaction. Results: Compared with the model group, the bone tissue structure and imbalance of bone metabolism were improved, and the bone mineral density was significantly increased in the prepared Radix Rehmanniainon combined with Radix Astragali groups. After intervention with prepared Radix Rehmanniainon combined with Radix Astragali, the positive expression of PIK3CA and Akt1 in rat bone tissue was enhanced, and the expression levels of Akt1 mRNA were significantly increased. Conclusions: Prepared Radix Rehmanniainon combined with Radix Astragali may treat osteoporosis by activating PI3K/AKT pathway.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001100200&tlng=enOsteoporosisBone and BonesRats |
| spellingShingle | Wen-qian Kang Pei-feng Wei Li Ou Min Li Chun-yu Liu The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway Acta Cirúrgica Brasileira Osteoporosis Bone and Bones Rats |
| title | The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway |
| title_full | The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway |
| title_fullStr | The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway |
| title_full_unstemmed | The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway |
| title_short | The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway |
| title_sort | mechanism study of inhibition effect of prepared radix rehmanniainon combined with radix astragali osteoporosis through pi3k akt signaling pathway |
| topic | Osteoporosis Bone and Bones Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001100200&tlng=en |
| work_keys_str_mv | AT wenqiankang themechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT peifengwei themechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT liou themechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT minli themechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT chunyuliu themechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT wenqiankang mechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT peifengwei mechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT liou mechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT minli mechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway AT chunyuliu mechanismstudyofinhibitioneffectofpreparedradixrehmanniainoncombinedwithradixastragaliosteoporosisthroughpi3kaktsignalingpathway |