Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in Rats
Introduction: Methamphetamine (METH) is a neurotoxic psychostimulant with highly addictive potential that leads to compulsive drug use and vulnerability to relapse. Environmental cues, such as drug exposure, peer influence, and social stress, are the powerful triggers of drug relapse. In this study,...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2018-05-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-107-2&slc_lang=en&sid=1 |
| _version_ | 1797284987068743680 |
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| author | Zahra Taslimi Alireza Komaki Abbas Haghparast Abdolrahman Sarihi |
| author_facet | Zahra Taslimi Alireza Komaki Abbas Haghparast Abdolrahman Sarihi |
| author_sort | Zahra Taslimi |
| collection | DOAJ |
| description | Introduction: Methamphetamine (METH) is a neurotoxic psychostimulant with highly addictive potential that leads to compulsive drug use and vulnerability to relapse. Environmental cues, such as drug exposure, peer influence, and social stress, are the powerful triggers of drug relapse. In this study,.we.tried.to.find.out.the effect of acute and chronic restraint stress on reinstatement of extinguished METH-induced Conditioned Place.Preference.(CPP).in.rats.
Methods: Subcutaneous (SC) administration of METH (0.125, 0.25, 0.5, 1, 2 and 4 mg/kg) could induce CPP and it was found that.METH with the dose of 0.5 mg/kg was more potent than other doses. In.extinction phase,.rats.were.put.in.the.CPP.box.for.30.min.per.day.for.8 consecutive days..After.extinction,.animals.were.exposed.to restraint stress (3-h period, as an acute stress) 60.min.before.subcutaneous.administration.of.ineffective.dose.of.METH.(0.125.mg/kg).in.order.to.reinstate.the.extinguished.METH-induced CPP. For induction of the chronic stress during extinction phase, the animals were exposed to the restraint stress for one hour per day.
Results: The results showed that the effective dose of METH to induce CPP was 0.5 mg/kg..Based on the results, physical.stress.(restraint stress) whether acute and chronic, can.significantly.induce.reinstatement.of METH-induced CPP (P˂0.001) in extinguished animals.
Conclusion: Additionally, the chronic restraint stress could reduce duration of extinction (maintenance) of METH-induced CPP. It seems that exposure to the stress induces the relapse in abstinent amphetamine, but acute and chronic situation have a different reaction. |
| first_indexed | 2024-03-07T17:55:41Z |
| format | Article |
| id | doaj.art-47304e233ecf44ab9a4590aaa8806a3a |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:55:41Z |
| publishDate | 2018-05-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-47304e233ecf44ab9a4590aaa8806a3a2024-03-02T12:14:09ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422018-05-0193157166Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in RatsZahra Taslimi0Alireza Komaki1Abbas Haghparast2Abdolrahman Sarihi3 Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Introduction: Methamphetamine (METH) is a neurotoxic psychostimulant with highly addictive potential that leads to compulsive drug use and vulnerability to relapse. Environmental cues, such as drug exposure, peer influence, and social stress, are the powerful triggers of drug relapse. In this study,.we.tried.to.find.out.the effect of acute and chronic restraint stress on reinstatement of extinguished METH-induced Conditioned Place.Preference.(CPP).in.rats. Methods: Subcutaneous (SC) administration of METH (0.125, 0.25, 0.5, 1, 2 and 4 mg/kg) could induce CPP and it was found that.METH with the dose of 0.5 mg/kg was more potent than other doses. In.extinction phase,.rats.were.put.in.the.CPP.box.for.30.min.per.day.for.8 consecutive days..After.extinction,.animals.were.exposed.to restraint stress (3-h period, as an acute stress) 60.min.before.subcutaneous.administration.of.ineffective.dose.of.METH.(0.125.mg/kg).in.order.to.reinstate.the.extinguished.METH-induced CPP. For induction of the chronic stress during extinction phase, the animals were exposed to the restraint stress for one hour per day. Results: The results showed that the effective dose of METH to induce CPP was 0.5 mg/kg..Based on the results, physical.stress.(restraint stress) whether acute and chronic, can.significantly.induce.reinstatement.of METH-induced CPP (P˂0.001) in extinguished animals. Conclusion: Additionally, the chronic restraint stress could reduce duration of extinction (maintenance) of METH-induced CPP. It seems that exposure to the stress induces the relapse in abstinent amphetamine, but acute and chronic situation have a different reaction.http://bcn.iums.ac.ir/browse.php?a_code=A-10-107-2&slc_lang=en&sid=1Reward Stress Methamphetamine (METH) Reinstatement Conditioned place preference |
| spellingShingle | Zahra Taslimi Alireza Komaki Abbas Haghparast Abdolrahman Sarihi Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in Rats Basic and Clinical Neuroscience Reward Stress Methamphetamine (METH) Reinstatement Conditioned place preference |
| title | Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in Rats |
| title_full | Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in Rats |
| title_fullStr | Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in Rats |
| title_full_unstemmed | Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in Rats |
| title_short | Effects of Acute and Chronic Restraint Stress on Reinstatement of Extinguished Methamphetamine-induced Conditioned Place Preference in Rats |
| title_sort | effects of acute and chronic restraint stress on reinstatement of extinguished methamphetamine induced conditioned place preference in rats |
| topic | Reward Stress Methamphetamine (METH) Reinstatement Conditioned place preference |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-107-2&slc_lang=en&sid=1 |
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