Effect of gamma radiation on the growth of Nigella sativa L. callus
ABSTRACT<br /> The effect of different doses of gamma rays on the fresh weight and some cell constituents of Black seed (Nigella sativa L.) stem callus was investigated. <br /> The results revealed an increase in the fresh weight with an increase in the cellular contents of protein and n...
| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
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College of Education for Pure Sciences
2009-06-01
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| Series: | مجلة التربية والعلم |
| Subjects: | |
| Online Access: | https://edusj.mosuljournals.com/article_57667_9f6bceb3121f85881ae00ed2372f11eb.pdf |
| _version_ | 1819035144709734400 |
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| author | Hekmat Mustafa Al Doliemy Sajeda Aziz Aboud |
| author_facet | Hekmat Mustafa Al Doliemy Sajeda Aziz Aboud |
| author_sort | Hekmat Mustafa Al Doliemy |
| collection | DOAJ |
| description | ABSTRACT<br /> The effect of different doses of gamma rays on the fresh weight and some cell constituents of Black seed (Nigella sativa L.) stem callus was investigated. <br /> The results revealed an increase in the fresh weight with an increase in the cellular contents of protein and nucleic acid (DNA, RNA) extracted from radiated callus at doses 5,10, 30 and 50 rad, on the other hand, the high doses (70, 90 rad) led to death more than of 50% of initiating cells of callus. <br /> Shoots formation occurred on radiated callus, at dose 50 rad. In solid MS media, which are provided with 2,4–D(10-6 M). Verdure branches that occurred on radiated callus have been rooted by using MS free growth regulators. The plant developed on this medium can be readly transfered to the soil after harding. |
| first_indexed | 2024-12-21T07:44:58Z |
| format | Article |
| id | doaj.art-473093400bee417e818ec7fd9d9eb4c4 |
| institution | Directory Open Access Journal |
| issn | 1812-125X 2664-2530 |
| language | Arabic |
| last_indexed | 2024-12-21T07:44:58Z |
| publishDate | 2009-06-01 |
| publisher | College of Education for Pure Sciences |
| record_format | Article |
| series | مجلة التربية والعلم |
| spelling | doaj.art-473093400bee417e818ec7fd9d9eb4c42022-12-21T19:11:14ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302009-06-01222919910.33899/edusj.2009.5766757667Effect of gamma radiation on the growth of Nigella sativa L. callusHekmat Mustafa Al DoliemySajeda Aziz AboudABSTRACT<br /> The effect of different doses of gamma rays on the fresh weight and some cell constituents of Black seed (Nigella sativa L.) stem callus was investigated. <br /> The results revealed an increase in the fresh weight with an increase in the cellular contents of protein and nucleic acid (DNA, RNA) extracted from radiated callus at doses 5,10, 30 and 50 rad, on the other hand, the high doses (70, 90 rad) led to death more than of 50% of initiating cells of callus. <br /> Shoots formation occurred on radiated callus, at dose 50 rad. In solid MS media, which are provided with 2,4–D(10-6 M). Verdure branches that occurred on radiated callus have been rooted by using MS free growth regulators. The plant developed on this medium can be readly transfered to the soil after harding.https://edusj.mosuljournals.com/article_57667_9f6bceb3121f85881ae00ed2372f11eb.pdfgamma radiationnigella sativa lcallus |
| spellingShingle | Hekmat Mustafa Al Doliemy Sajeda Aziz Aboud Effect of gamma radiation on the growth of Nigella sativa L. callus مجلة التربية والعلم gamma radiation nigella sativa l callus |
| title | Effect of gamma radiation on the growth of Nigella sativa L. callus |
| title_full | Effect of gamma radiation on the growth of Nigella sativa L. callus |
| title_fullStr | Effect of gamma radiation on the growth of Nigella sativa L. callus |
| title_full_unstemmed | Effect of gamma radiation on the growth of Nigella sativa L. callus |
| title_short | Effect of gamma radiation on the growth of Nigella sativa L. callus |
| title_sort | effect of gamma radiation on the growth of nigella sativa l callus |
| topic | gamma radiation nigella sativa l callus |
| url | https://edusj.mosuljournals.com/article_57667_9f6bceb3121f85881ae00ed2372f11eb.pdf |
| work_keys_str_mv | AT hekmatmustafaaldoliemy effectofgammaradiationonthegrowthofnigellasativalcallus AT sajedaazizaboud effectofgammaradiationonthegrowthofnigellasativalcallus |