Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a deca...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1101232/full |
| _version_ | 1797952058980040704 |
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| author | Abhroop Garg Carsten Jers Hee Jin Hwang Hee Jin Hwang Aida Kalantari Ildze Ventina Ivan Mijakovic Ivan Mijakovic |
| author_facet | Abhroop Garg Carsten Jers Hee Jin Hwang Hee Jin Hwang Aida Kalantari Ildze Ventina Ivan Mijakovic Ivan Mijakovic |
| author_sort | Abhroop Garg |
| collection | DOAJ |
| description | 3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP. |
| first_indexed | 2024-04-10T22:40:25Z |
| format | Article |
| id | doaj.art-4732a102545e447abae8122b9ff2cadf |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T22:40:25Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-4732a102545e447abae8122b9ff2cadf2023-01-16T05:22:08ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011110.3389/fbioe.2023.11012321101232Engineering Bacillus subtilis for production of 3-hydroxypropanoic acidAbhroop Garg0Carsten Jers1Hee Jin Hwang2Hee Jin Hwang3Aida Kalantari4Ildze Ventina5Ivan Mijakovic6Ivan Mijakovic7Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, DenmarkSystems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SwedenDepartment of Molecular Science and Technology, Ajou University, World cup-ro, Yeongtong-gu, Suwon-si, South KoreaSystems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SwedenNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, DenmarkSystems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1101232/full3-hydroxypropanoic acidglycerolbiosynthesiscell factorysynthetic biologymetabolic engineering |
| spellingShingle | Abhroop Garg Carsten Jers Hee Jin Hwang Hee Jin Hwang Aida Kalantari Ildze Ventina Ivan Mijakovic Ivan Mijakovic Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid Frontiers in Bioengineering and Biotechnology 3-hydroxypropanoic acid glycerol biosynthesis cell factory synthetic biology metabolic engineering |
| title | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
| title_full | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
| title_fullStr | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
| title_full_unstemmed | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
| title_short | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
| title_sort | engineering bacillus subtilis for production of 3 hydroxypropanoic acid |
| topic | 3-hydroxypropanoic acid glycerol biosynthesis cell factory synthetic biology metabolic engineering |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1101232/full |
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