Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG
Guanyang Chen,1,* Zhehong Li,2,* Qing Sang,1,* Liang Wang,2 Qiqige Wuyun,2 Zheng Wang,2 Weijian Chen,2 Chengyuan Yu,1 Dongbo Lian,2 Nengwei Zhang2 1Department of General Surgery, Peking University Ninth School of Clinical Medicine, Beijing, People’s Republic of China; 2Depart...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-05-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
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| Online Access: | https://www.dovepress.com/establishment-of-a-nomogram-based-on-inflammatory-response-related-met-peer-reviewed-fulltext-article-DMSO |
| _version_ | 1797830159209857024 |
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| author | Chen G Li Z Sang Q Wang L Wuyun Q Wang Z Chen W Yu C Lian D Zhang N |
| author_facet | Chen G Li Z Sang Q Wang L Wuyun Q Wang Z Chen W Yu C Lian D Zhang N |
| author_sort | Chen G |
| collection | DOAJ |
| description | Guanyang Chen,1,* Zhehong Li,2,* Qing Sang,1,* Liang Wang,2 Qiqige Wuyun,2 Zheng Wang,2 Weijian Chen,2 Chengyuan Yu,1 Dongbo Lian,2 Nengwei Zhang2 1Department of General Surgery, Peking University Ninth School of Clinical Medicine, Beijing, People’s Republic of China; 2Department of General Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dongbo Lian; Nengwei Zhang, Email liandongbo2478@bjsjth.cn; znw@bjsjth.cnBackground: Laparoscopic sleeve gastrectomy (LSG) is considered as an effective bariatric and metabolic surgery for patients with severe obesity. Chronic low-grade inflammation of adipose tissue is associated with obesity and obesity-related complications.Objective: This study intends to establish a nomogram based on inflammatory response-related methylation sites in intraoperative visceral adipose tissue (VAT) to predict excess weight loss (EWL)% at one-year after LSG.Methods: Based on EWL% at one-year after LSG, patients were divided into two groups: the satisfied group (group-A, EWL%≥ 50%) and the unsatisfied group (group-B, EWL%< 50%). Next, we defined genes corresponding to the methylation sites in the 850 K methylation microarray as methylation-related genes (MRGs). We then took the intersection of MRGs and inflammatory response-related genes. After that, inflammatory response-related methylation sites were identified based on overlapping genes. Moreover, difference analysis was carried out to obtain inflammatory response-related differentially methylated sites (IRRDMSs) between group-A and group-B. LASSO analysis was used to identify the hub methylation sites. Finally, we developed a nomogram based on the hub methylation sites.Results: There were 26 patients in the study, with 13 in group-A and 13 in group-B. After data filtering and difference analysis, 200 IRRDMSs were identified (143 hypermethylated sites and 57 hypomethylated sites). Then, we identified three hub methylation sites (cg03610073, cg03208951, and cg18746357) by LASSO analysis and built a predictive nomogram (Area under the curve=0.953).Conclusion: The predictive nomogram based on three inflammatory-related methylation sites (cg03610073, cg03208951, and cg18746357) in intraoperative visceral adipose tissue can predict one-year EWL% after LSG effectively.Keywords: DNA methylation, excess weight loss, laparoscopic sleeve gastrectomy, inflammation, nomogram |
| first_indexed | 2024-04-09T13:32:42Z |
| format | Article |
| id | doaj.art-4733e013d9e34cb380339aae7f9351dc |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-09T13:32:42Z |
| publishDate | 2023-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-4733e013d9e34cb380339aae7f9351dc2023-05-09T18:57:55ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072023-05-01Volume 161335134583573Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSGChen GLi ZSang QWang LWuyun QWang ZChen WYu CLian DZhang NGuanyang Chen,1,* Zhehong Li,2,* Qing Sang,1,* Liang Wang,2 Qiqige Wuyun,2 Zheng Wang,2 Weijian Chen,2 Chengyuan Yu,1 Dongbo Lian,2 Nengwei Zhang2 1Department of General Surgery, Peking University Ninth School of Clinical Medicine, Beijing, People’s Republic of China; 2Department of General Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dongbo Lian; Nengwei Zhang, Email liandongbo2478@bjsjth.cn; znw@bjsjth.cnBackground: Laparoscopic sleeve gastrectomy (LSG) is considered as an effective bariatric and metabolic surgery for patients with severe obesity. Chronic low-grade inflammation of adipose tissue is associated with obesity and obesity-related complications.Objective: This study intends to establish a nomogram based on inflammatory response-related methylation sites in intraoperative visceral adipose tissue (VAT) to predict excess weight loss (EWL)% at one-year after LSG.Methods: Based on EWL% at one-year after LSG, patients were divided into two groups: the satisfied group (group-A, EWL%≥ 50%) and the unsatisfied group (group-B, EWL%< 50%). Next, we defined genes corresponding to the methylation sites in the 850 K methylation microarray as methylation-related genes (MRGs). We then took the intersection of MRGs and inflammatory response-related genes. After that, inflammatory response-related methylation sites were identified based on overlapping genes. Moreover, difference analysis was carried out to obtain inflammatory response-related differentially methylated sites (IRRDMSs) between group-A and group-B. LASSO analysis was used to identify the hub methylation sites. Finally, we developed a nomogram based on the hub methylation sites.Results: There were 26 patients in the study, with 13 in group-A and 13 in group-B. After data filtering and difference analysis, 200 IRRDMSs were identified (143 hypermethylated sites and 57 hypomethylated sites). Then, we identified three hub methylation sites (cg03610073, cg03208951, and cg18746357) by LASSO analysis and built a predictive nomogram (Area under the curve=0.953).Conclusion: The predictive nomogram based on three inflammatory-related methylation sites (cg03610073, cg03208951, and cg18746357) in intraoperative visceral adipose tissue can predict one-year EWL% after LSG effectively.Keywords: DNA methylation, excess weight loss, laparoscopic sleeve gastrectomy, inflammation, nomogramhttps://www.dovepress.com/establishment-of-a-nomogram-based-on-inflammatory-response-related-met-peer-reviewed-fulltext-article-DMSOdna methylationexcess weight losslaparoscopic sleeve gastrectomyinflammationnomogram. |
| spellingShingle | Chen G Li Z Sang Q Wang L Wuyun Q Wang Z Chen W Yu C Lian D Zhang N Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG Diabetes, Metabolic Syndrome and Obesity dna methylation excess weight loss laparoscopic sleeve gastrectomy inflammation nomogram. |
| title | Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG |
| title_full | Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG |
| title_fullStr | Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG |
| title_full_unstemmed | Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG |
| title_short | Establishment of a Nomogram Based on Inflammatory Response-Related Methylation Sites in Intraoperative Visceral Adipose Tissue to Predict EWL% at One Year After LSG |
| title_sort | establishment of a nomogram based on inflammatory response related methylation sites in intraoperative visceral adipose tissue to predict ewl at one year after lsg |
| topic | dna methylation excess weight loss laparoscopic sleeve gastrectomy inflammation nomogram. |
| url | https://www.dovepress.com/establishment-of-a-nomogram-based-on-inflammatory-response-related-met-peer-reviewed-fulltext-article-DMSO |
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