Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo
The high mortality rate caused by atherosclerosis makes it necessary to constantly search for new and better treatments. In previous reports, chemically modified carbon-coated iron nanoparticles (Fe@C NPs) have been demonstrated a high biocompatibility and promising anti-plaque properties. To furthe...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8241 |
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| author | Shamil Akhmedov Sergey Afanasyev Natalia Beshchasna Marina Trusova Ivan Stepanov Mariya Rebenkova Ekaterina Poletykina Yuri Vecherskiy Sergei Tverdokhlebov Evgeny Bolbasov Sascha Balakin Joerg Opitz Anatoly Yermakov Boris Kozlov |
| author_facet | Shamil Akhmedov Sergey Afanasyev Natalia Beshchasna Marina Trusova Ivan Stepanov Mariya Rebenkova Ekaterina Poletykina Yuri Vecherskiy Sergei Tverdokhlebov Evgeny Bolbasov Sascha Balakin Joerg Opitz Anatoly Yermakov Boris Kozlov |
| author_sort | Shamil Akhmedov |
| collection | DOAJ |
| description | The high mortality rate caused by atherosclerosis makes it necessary to constantly search for new and better treatments. In previous reports, chemically modified carbon-coated iron nanoparticles (Fe@C NPs) have been demonstrated a high biocompatibility and promising anti-plaque properties. To further investigate these effects, the interaction of these nanoparticles with the adipose tissue of Wistar rats (in vivo) and human atherosclerotic plaques (ex vivo) was studied. For the in vivo study, cobalt–chromium (CoCr) alloy tubes, which are used for coronary stent manufacturing, were prepared with a coating of polylactic acid (PLA) which contained either modified or non-modified Fe@C NPs in a 5% by weight concentration. The tubes were implanted into an area of subcutaneous fat in Wistar rats, where changes in the histological structure and functional properties of the surrounding tissue were observed in the case of coatings modified with Fe@C NPs. For the ex vivo study, freshly explanted human atherosclerotic plaques were treated in the physiological solution with doses of modified Fe@C NPs, with mass equal to 5% or 25% relative to the plaques. This treatment resulted in the release of cholesterol-like compounds from the surface of the plaques into the solution, thus proving a pronounced destructive effect on the plaque structure. Chemically modified Fe@C NPs, when used as an anti-atherosclerosis agent, were able to activate the activity of macrophages, which could lead to the destruction of atherosclerotic plaques structures. These findings could prove the fabrication of next-generation vascular stents with built-in anti-atherosclerotic agents. |
| first_indexed | 2024-03-09T05:22:02Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:22:02Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-473eb45b63b14206b555852f3b8aca062023-12-03T12:39:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315824110.3390/ijms23158241Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In VivoShamil Akhmedov0Sergey Afanasyev1Natalia Beshchasna2Marina Trusova3Ivan Stepanov4Mariya Rebenkova5Ekaterina Poletykina6Yuri Vecherskiy7Sergei Tverdokhlebov8Evgeny Bolbasov9Sascha Balakin10Joerg Opitz11Anatoly Yermakov12Boris Kozlov13Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634012 Tomsk, RussiaCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634012 Tomsk, RussiaFraunhofer Institute for Ceramic Technologies and Systems IKTS, 01109 Dresden, GermanyResearch School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, 634050 Tomsk, RussiaCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634012 Tomsk, RussiaCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634012 Tomsk, RussiaResearch School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, 634050 Tomsk, RussiaCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634012 Tomsk, RussiaSchool of Nuclear Science and Engineering, Tomsk Polytechnic University, 634050 Tomsk, RussiaSchool of Nuclear Science and Engineering, Tomsk Polytechnic University, 634050 Tomsk, RussiaFraunhofer Institute for Ceramic Technologies and Systems IKTS, 01109 Dresden, GermanyFraunhofer Institute for Ceramic Technologies and Systems IKTS, 01109 Dresden, GermanyM.N. Miheev Institute of Metal Physics of Ural Branch of Russian Academy of Sciences, 620108 Ekaterinburg, RussiaCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634012 Tomsk, RussiaThe high mortality rate caused by atherosclerosis makes it necessary to constantly search for new and better treatments. In previous reports, chemically modified carbon-coated iron nanoparticles (Fe@C NPs) have been demonstrated a high biocompatibility and promising anti-plaque properties. To further investigate these effects, the interaction of these nanoparticles with the adipose tissue of Wistar rats (in vivo) and human atherosclerotic plaques (ex vivo) was studied. For the in vivo study, cobalt–chromium (CoCr) alloy tubes, which are used for coronary stent manufacturing, were prepared with a coating of polylactic acid (PLA) which contained either modified or non-modified Fe@C NPs in a 5% by weight concentration. The tubes were implanted into an area of subcutaneous fat in Wistar rats, where changes in the histological structure and functional properties of the surrounding tissue were observed in the case of coatings modified with Fe@C NPs. For the ex vivo study, freshly explanted human atherosclerotic plaques were treated in the physiological solution with doses of modified Fe@C NPs, with mass equal to 5% or 25% relative to the plaques. This treatment resulted in the release of cholesterol-like compounds from the surface of the plaques into the solution, thus proving a pronounced destructive effect on the plaque structure. Chemically modified Fe@C NPs, when used as an anti-atherosclerosis agent, were able to activate the activity of macrophages, which could lead to the destruction of atherosclerotic plaques structures. These findings could prove the fabrication of next-generation vascular stents with built-in anti-atherosclerotic agents.https://www.mdpi.com/1422-0067/23/15/8241atherosclerosisatherosclerotic plaquechemically modified carbon-coated iron nanoparticlereverse cholesterol transportmacrophagecoronary stent |
| spellingShingle | Shamil Akhmedov Sergey Afanasyev Natalia Beshchasna Marina Trusova Ivan Stepanov Mariya Rebenkova Ekaterina Poletykina Yuri Vecherskiy Sergei Tverdokhlebov Evgeny Bolbasov Sascha Balakin Joerg Opitz Anatoly Yermakov Boris Kozlov Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo International Journal of Molecular Sciences atherosclerosis atherosclerotic plaque chemically modified carbon-coated iron nanoparticle reverse cholesterol transport macrophage coronary stent |
| title | Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo |
| title_full | Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo |
| title_fullStr | Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo |
| title_full_unstemmed | Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo |
| title_short | Effect of Chemically Modified Carbon-Coated Iron Nanoparticles on the Structure of Human Atherosclerotic Plaques Ex Vivo and on Adipose Tissue in Chronic Experiment In Vivo |
| title_sort | effect of chemically modified carbon coated iron nanoparticles on the structure of human atherosclerotic plaques ex vivo and on adipose tissue in chronic experiment in vivo |
| topic | atherosclerosis atherosclerotic plaque chemically modified carbon-coated iron nanoparticle reverse cholesterol transport macrophage coronary stent |
| url | https://www.mdpi.com/1422-0067/23/15/8241 |
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