Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum
Summary Currently, piperazic acid is chemically synthesized using ecologically unfriendly processes. Microbial synthesis from glucose is an attractive alternative to chemical synthesis. In this study, we report the production of L‐piperazic acid via microbial fermentation with the first engineered f...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-07-01
|
| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13838 |
| _version_ | 1818934355621314560 |
|---|---|
| author | Cuncui Kong Zhuangzhuang Wang Guanglei Liu Zhenming Chi Rodrigo Ledesma‐Amaro Zhe Chi |
| author_facet | Cuncui Kong Zhuangzhuang Wang Guanglei Liu Zhenming Chi Rodrigo Ledesma‐Amaro Zhe Chi |
| author_sort | Cuncui Kong |
| collection | DOAJ |
| description | Summary Currently, piperazic acid is chemically synthesized using ecologically unfriendly processes. Microbial synthesis from glucose is an attractive alternative to chemical synthesis. In this study, we report the production of L‐piperazic acid via microbial fermentation with the first engineered fungal strain of Aureobasidium melanogenum; this strain was constructed by chassis development, genetic element reconstitution and optimization, synthetic rewiring and constitutive genetic circuit reconstitution, to build a robust L‐piperazic acid synthetic cascade. These genetic modifications enable A. melanogenum to directly convert glucose to L‐piperazic acid without relying on the use of either chemically synthesized precursors or harsh conditions. This bio‐based process overcomes the shortcomings of the conventional synthesis routes. The ultimately engineered strain is a very high‐efficient cell factory that can excrete 1.12 ± 0.05 g l‐1 of L‐piperazic acid after a 120‐h 10.0‐l fed‐batch fermentation; this is the highest titre of L‐piperazic acid reported using a microbial cell factory. |
| first_indexed | 2024-12-20T05:02:58Z |
| format | Article |
| id | doaj.art-4740009c815643689594ccce39594a21 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-20T05:02:58Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-4740009c815643689594ccce39594a212022-12-21T19:52:30ZengWileyMicrobial Biotechnology1751-79152021-07-011441722172910.1111/1751-7915.13838Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenumCuncui Kong0Zhuangzhuang Wang1Guanglei Liu2Zhenming Chi3Rodrigo Ledesma‐Amaro4Zhe Chi5College of Marine Life Sciences Ocean University of China No.5 Yushan Road Qingdao 266003 ChinaCollege of Marine Life Sciences Ocean University of China No.5 Yushan Road Qingdao 266003 ChinaCollege of Marine Life Sciences Ocean University of China No.5 Yushan Road Qingdao 266003 ChinaCollege of Marine Life Sciences Ocean University of China No.5 Yushan Road Qingdao 266003 ChinaDepartment of Bioengineering Imperial College London London SW7 2AZ UKCollege of Marine Life Sciences Ocean University of China No.5 Yushan Road Qingdao 266003 ChinaSummary Currently, piperazic acid is chemically synthesized using ecologically unfriendly processes. Microbial synthesis from glucose is an attractive alternative to chemical synthesis. In this study, we report the production of L‐piperazic acid via microbial fermentation with the first engineered fungal strain of Aureobasidium melanogenum; this strain was constructed by chassis development, genetic element reconstitution and optimization, synthetic rewiring and constitutive genetic circuit reconstitution, to build a robust L‐piperazic acid synthetic cascade. These genetic modifications enable A. melanogenum to directly convert glucose to L‐piperazic acid without relying on the use of either chemically synthesized precursors or harsh conditions. This bio‐based process overcomes the shortcomings of the conventional synthesis routes. The ultimately engineered strain is a very high‐efficient cell factory that can excrete 1.12 ± 0.05 g l‐1 of L‐piperazic acid after a 120‐h 10.0‐l fed‐batch fermentation; this is the highest titre of L‐piperazic acid reported using a microbial cell factory.https://doi.org/10.1111/1751-7915.13838 |
| spellingShingle | Cuncui Kong Zhuangzhuang Wang Guanglei Liu Zhenming Chi Rodrigo Ledesma‐Amaro Zhe Chi Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum Microbial Biotechnology |
| title | Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum |
| title_full | Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum |
| title_fullStr | Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum |
| title_full_unstemmed | Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum |
| title_short | Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum |
| title_sort | bioproduction of l piperazic acid in gram scale using aureobasidium melanogenum |
| url | https://doi.org/10.1111/1751-7915.13838 |
| work_keys_str_mv | AT cuncuikong bioproductionoflpiperazicacidingramscaleusingaureobasidiummelanogenum AT zhuangzhuangwang bioproductionoflpiperazicacidingramscaleusingaureobasidiummelanogenum AT guangleiliu bioproductionoflpiperazicacidingramscaleusingaureobasidiummelanogenum AT zhenmingchi bioproductionoflpiperazicacidingramscaleusingaureobasidiummelanogenum AT rodrigoledesmaamaro bioproductionoflpiperazicacidingramscaleusingaureobasidiummelanogenum AT zhechi bioproductionoflpiperazicacidingramscaleusingaureobasidiummelanogenum |