Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4
Ashy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The ob...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-11-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1019263/full |
| _version_ | 1811189069106905088 |
|---|---|
| author | Diego M. Viteri Angela M. Linares Zoralys Miranda Ainong Shi |
| author_facet | Diego M. Viteri Angela M. Linares Zoralys Miranda Ainong Shi |
| author_sort | Diego M. Viteri |
| collection | DOAJ |
| description | Ashy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The objective of this research was to identify QTL and single nucleotide polymorphism (SNP) markers associated with ASB resistance in recombinant inbred lines (RIL) derived from a cross between BAT 477 and NY6020-4 common bean. One hundred and twenty-six F6:7 RIL were phenotyped for ASB in the greenhouse. Disease severity was scored on a scale of 1–9. Genotyping was performed using whole genome resequencing with 2x common bean genome size coverage, and over six million SNPs were obtained. After being filtered, 72,017 SNPs distributed on 11 chromosomes were used to conduct the genome-wide association study (GWAS) and QTL mapping. A novel QTL region of ~4.28 Mbp from 35,546,329 bp to 39,826,434 bp on chromosome Pv03 was identified for ASB resistance. The two SNPs, Chr03_39824257 and Chr03_39824268 located at 39,824,257 bp and 39,824,268 bp on Pv03, respectively, were identified as the strongest markers associated with ASB resistance. The gene Phvul.003G175900 (drought sensitive, WD repeat-containing protein 76) located at 39,822,021 – 39,824,655 bp on Pv03 was recognized as one candidate for ASB resistance in the RIL, and the gene contained the two SNP markers. QTL and SNP markers may be used to select plants and lines for ASB resistance through marker-assisted selection (MAS) in common bean breeding. |
| first_indexed | 2024-04-11T14:29:42Z |
| format | Article |
| id | doaj.art-47457145132c49ee8cfb0addedbc14b6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T14:29:42Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-47457145132c49ee8cfb0addedbc14b62022-12-22T04:18:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10192631019263Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4Diego M. Viteri0Angela M. Linares1Zoralys Miranda2Ainong Shi3Department of Agro-environmental Sciences, University of Puerto Rico, Isabela Research Substation, Isabela, PR, United StatesDepartment of Agro-environmental Sciences, University of Puerto Rico, Lajas Research Substation, Lajas, PR, United StatesDepartment of Agro-environmental Sciences, University of Puerto Rico, Isabela Research Substation, Isabela, PR, United StatesDepartment of Horticulture, University of Arkansas, Fayetteville, AR, United StatesAshy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The objective of this research was to identify QTL and single nucleotide polymorphism (SNP) markers associated with ASB resistance in recombinant inbred lines (RIL) derived from a cross between BAT 477 and NY6020-4 common bean. One hundred and twenty-six F6:7 RIL were phenotyped for ASB in the greenhouse. Disease severity was scored on a scale of 1–9. Genotyping was performed using whole genome resequencing with 2x common bean genome size coverage, and over six million SNPs were obtained. After being filtered, 72,017 SNPs distributed on 11 chromosomes were used to conduct the genome-wide association study (GWAS) and QTL mapping. A novel QTL region of ~4.28 Mbp from 35,546,329 bp to 39,826,434 bp on chromosome Pv03 was identified for ASB resistance. The two SNPs, Chr03_39824257 and Chr03_39824268 located at 39,824,257 bp and 39,824,268 bp on Pv03, respectively, were identified as the strongest markers associated with ASB resistance. The gene Phvul.003G175900 (drought sensitive, WD repeat-containing protein 76) located at 39,822,021 – 39,824,655 bp on Pv03 was recognized as one candidate for ASB resistance in the RIL, and the gene contained the two SNP markers. QTL and SNP markers may be used to select plants and lines for ASB resistance through marker-assisted selection (MAS) in common bean breeding.https://www.frontiersin.org/articles/10.3389/fpls.2022.1019263/fullashy stem blightcommon beanMacrophomina phaseolinaPhaseolus vulgarisquantitative trait locigenome-wide association study |
| spellingShingle | Diego M. Viteri Angela M. Linares Zoralys Miranda Ainong Shi Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4 Frontiers in Plant Science ashy stem blight common bean Macrophomina phaseolina Phaseolus vulgaris quantitative trait loci genome-wide association study |
| title | Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4 |
| title_full | Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4 |
| title_fullStr | Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4 |
| title_full_unstemmed | Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4 |
| title_short | Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4 |
| title_sort | identification of a qtl region for ashy stem blight resistance using genome wide association and linage analysis in common bean recombinant inbred lines derived from bat 477 and ny6020 4 |
| topic | ashy stem blight common bean Macrophomina phaseolina Phaseolus vulgaris quantitative trait loci genome-wide association study |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1019263/full |
| work_keys_str_mv | AT diegomviteri identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204 AT angelamlinares identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204 AT zoralysmiranda identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204 AT ainongshi identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204 |