The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri
Glutathione S-transferase (GST) is a superfamily of multifunction enzymes with varying catalytic roles in cellular detoxification to protect hosts against oxidative damage. In the present study, six GST genes were identified from Chlamys farreri, including CfGSTω, CfGSTσ-1, CfGSTσ-2, CfGSTρ, CfGSTζ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2018-08-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/439 |
| _version_ | 1818965678120501248 |
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| author | M Wang L Wang D Ni Q Yi X Wang Z Jia L Song |
| author_facet | M Wang L Wang D Ni Q Yi X Wang Z Jia L Song |
| author_sort | M Wang |
| collection | DOAJ |
| description | Glutathione S-transferase (GST) is a superfamily of multifunction enzymes with varying catalytic
roles in cellular detoxification to protect hosts against oxidative damage. In the present study, six GST genes were identified from Chlamys farreri, including CfGSTω, CfGSTσ-1, CfGSTσ-2, CfGSTρ, CfGSTζ and CfmGST. CfGSTs shared high similarities with their counterparts from other species, and were clustered with their homologues into the corresponding clades in the phylogenetic tree, respectively. We investigated the distribution of their mRNA transcripts in different tissues and their temporal expression profiles in hemocytes after microbe stimulations by quantitative real-time PCR. The six CfGST genes were detectable in all the tested tissues, including hemocytes, muscle, mantle, gill, hepatopancreas, and gonad. Stimulations with various microbes drastically induced the mRNA transcripts of all the CfGSTs with different expression profiles. For examples, CfGSTω could be induced by three kinds of microbes, including Vibrio anguillarum, Micrococcus luteus and Pichia pastoris, whereas CfmGST could be only induced by V. anguillarum. These results indicated a powerful detoxification system of GSTs in scallop. Moreover, the distinct mRNA expression profiles of CfGSTs indicated their versatile and immune-challenge specific roles in the mollusk C. farreri. |
| first_indexed | 2024-12-20T13:20:49Z |
| format | Article |
| id | doaj.art-4746c658829b4a6a8a5e5dba39be49de |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-20T13:20:49Z |
| publishDate | 2018-08-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-4746c658829b4a6a8a5e5dba39be49de2022-12-21T19:39:24ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-08-01151The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreriM Wang0L Wang1D Ni2Q Yi3X Wang4Z Jia5L Song6CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China ; Dalian Key Laboratory of Disease Prevention and Control for Aquaculture Animals, Dalian Ocean University, Dalian 116023, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China ; Dalian Key Laboratory of Disease Prevention and Control for Aquaculture Animals, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao 266042, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Marine Science and Technology, Qingdao 266237, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China ; Dalian Key Laboratory of Disease Prevention and Control for Aquaculture Animals, Dalian Ocean University, Dalian 116023, ChinaGlutathione S-transferase (GST) is a superfamily of multifunction enzymes with varying catalytic roles in cellular detoxification to protect hosts against oxidative damage. In the present study, six GST genes were identified from Chlamys farreri, including CfGSTω, CfGSTσ-1, CfGSTσ-2, CfGSTρ, CfGSTζ and CfmGST. CfGSTs shared high similarities with their counterparts from other species, and were clustered with their homologues into the corresponding clades in the phylogenetic tree, respectively. We investigated the distribution of their mRNA transcripts in different tissues and their temporal expression profiles in hemocytes after microbe stimulations by quantitative real-time PCR. The six CfGST genes were detectable in all the tested tissues, including hemocytes, muscle, mantle, gill, hepatopancreas, and gonad. Stimulations with various microbes drastically induced the mRNA transcripts of all the CfGSTs with different expression profiles. For examples, CfGSTω could be induced by three kinds of microbes, including Vibrio anguillarum, Micrococcus luteus and Pichia pastoris, whereas CfmGST could be only induced by V. anguillarum. These results indicated a powerful detoxification system of GSTs in scallop. Moreover, the distinct mRNA expression profiles of CfGSTs indicated their versatile and immune-challenge specific roles in the mollusk C. farreri.https://isj02.unimore.it/index.php/ISJ/article/view/439Chlamys farreriGlutathione S-transferaseinnate immunity |
| spellingShingle | M Wang L Wang D Ni Q Yi X Wang Z Jia L Song The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri Invertebrate Survival Journal Chlamys farreri Glutathione S-transferase innate immunity |
| title | The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri |
| title_full | The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri |
| title_fullStr | The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri |
| title_full_unstemmed | The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri |
| title_short | The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri |
| title_sort | mrna expression profiles demonstrating versatile roles of glutathione s transferase genes in the mollusk chlamys farreri |
| topic | Chlamys farreri Glutathione S-transferase innate immunity |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/439 |
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