Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i>
The aim of this study was to determine the volatile phytochemical constituents and evaluate the antimicrobial activity of the essential oils of the leaves from <i>Croton adipatus</i>, <i>Croton thurifer</i>, and <i>Croton collinus</i>. Essential oils were extracte...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/11/1387 |
| _version_ | 1797511422202085376 |
|---|---|
| author | Juana Liz Leslie Cucho-Medrano Sammy Wesley Mendoza-Beingolea César Máximo Fuertes-Ruitón María Elena Salazar-Salvatierra Oscar Herrera-Calderon |
| author_facet | Juana Liz Leslie Cucho-Medrano Sammy Wesley Mendoza-Beingolea César Máximo Fuertes-Ruitón María Elena Salazar-Salvatierra Oscar Herrera-Calderon |
| author_sort | Juana Liz Leslie Cucho-Medrano |
| collection | DOAJ |
| description | The aim of this study was to determine the volatile phytochemical constituents and evaluate the antimicrobial activity of the essential oils of the leaves from <i>Croton adipatus</i>, <i>Croton thurifer</i>, and <i>Croton collinus</i>. Essential oils were extracted by hydro-distillation using the Clevenger extractor and the phytochemical analysis was determined by Gas chromatography-Mass Spectrometry (GC-MS). The antimicrobial activity was assessed using the agar diffusion and colorimetric broth microdilution methods against <i>Staphylococcus aureus</i> ATCC 25923, <i>Bacillus subtilis</i> ATCC 6633, <i>Escherichia coli</i> ATCC 25922, <i>Pseudomonas aeruginosa</i> ATCC 9027, and <i>Candida albicans</i> ATCC The essential oils from <i>C. adipatus</i>, <i>C. thurifer</i>, and <i>C collinus</i> had 46, 38, and 35 volatile constituents respectively. The main compounds determined in <i>C. adipatus</i> were β-myrcene (18.34%), while in <i>C. collinus</i> was β-caryophyllene (44.7%), and in <i>C. thurifer</i> was an unknown component (C<sub>10</sub>H<sub>16</sub>: 22.38%). Essential oil of <i>C. adipatus</i> showed a MIC against <i>B. subtilis</i> (286.4 µg/mL) and <i>C. albicans</i> (572.8 ± 0 µg/mL); <i>C. thurifer</i> against <i>S. aureus</i> (296.1 ± 0 µg/mL) and <i>B. subtilis</i> (148 ± 0 µg/mL); and <i>C. collinus</i> against <i>B. subtilis</i> (72 ± 0 µg/mL) and <i>C. albicans</i> (576.2 ± 0 µg/mL). The three essential oils of Croton species demonstrated <i>in vitro</i> antimicrobial activity against a strain of bacteria or fungi. |
| first_indexed | 2024-03-10T05:45:02Z |
| format | Article |
| id | doaj.art-474849a2e5654db1929dcb8aece9a71e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T05:45:02Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-474849a2e5654db1929dcb8aece9a71e2023-11-22T22:10:54ZengMDPI AGAntibiotics2079-63822021-11-011011138710.3390/antibiotics10111387Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i>Juana Liz Leslie Cucho-Medrano0Sammy Wesley Mendoza-Beingolea1César Máximo Fuertes-Ruitón2María Elena Salazar-Salvatierra3Oscar Herrera-Calderon4Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, PeruFaculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, PeruInstitute for Research in Pharmaceutical Sciences and Natural Resources, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, PeruResearch Institute in Biological Chemistry, Microbiology and Biotechnology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, PeruDepartment of Pharmacology, Bromatology and Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, PeruThe aim of this study was to determine the volatile phytochemical constituents and evaluate the antimicrobial activity of the essential oils of the leaves from <i>Croton adipatus</i>, <i>Croton thurifer</i>, and <i>Croton collinus</i>. Essential oils were extracted by hydro-distillation using the Clevenger extractor and the phytochemical analysis was determined by Gas chromatography-Mass Spectrometry (GC-MS). The antimicrobial activity was assessed using the agar diffusion and colorimetric broth microdilution methods against <i>Staphylococcus aureus</i> ATCC 25923, <i>Bacillus subtilis</i> ATCC 6633, <i>Escherichia coli</i> ATCC 25922, <i>Pseudomonas aeruginosa</i> ATCC 9027, and <i>Candida albicans</i> ATCC The essential oils from <i>C. adipatus</i>, <i>C. thurifer</i>, and <i>C collinus</i> had 46, 38, and 35 volatile constituents respectively. The main compounds determined in <i>C. adipatus</i> were β-myrcene (18.34%), while in <i>C. collinus</i> was β-caryophyllene (44.7%), and in <i>C. thurifer</i> was an unknown component (C<sub>10</sub>H<sub>16</sub>: 22.38%). Essential oil of <i>C. adipatus</i> showed a MIC against <i>B. subtilis</i> (286.4 µg/mL) and <i>C. albicans</i> (572.8 ± 0 µg/mL); <i>C. thurifer</i> against <i>S. aureus</i> (296.1 ± 0 µg/mL) and <i>B. subtilis</i> (148 ± 0 µg/mL); and <i>C. collinus</i> against <i>B. subtilis</i> (72 ± 0 µg/mL) and <i>C. albicans</i> (576.2 ± 0 µg/mL). The three essential oils of Croton species demonstrated <i>in vitro</i> antimicrobial activity against a strain of bacteria or fungi.https://www.mdpi.com/2079-6382/10/11/1387antimicrobialantifungalcolorimetric microdilutionvolatile oilmedicinal plantsgram positive |
| spellingShingle | Juana Liz Leslie Cucho-Medrano Sammy Wesley Mendoza-Beingolea César Máximo Fuertes-Ruitón María Elena Salazar-Salvatierra Oscar Herrera-Calderon Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i> Antibiotics antimicrobial antifungal colorimetric microdilution volatile oil medicinal plants gram positive |
| title | Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i> |
| title_full | Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i> |
| title_fullStr | Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i> |
| title_full_unstemmed | Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i> |
| title_short | Chemical Profile of the Volatile Constituents and Antimicrobial Activity of the Essential Oils from <i>Croton adipatus</i>, <i>Croton thurifer,</i> and <i>Croton collinus</i> |
| title_sort | chemical profile of the volatile constituents and antimicrobial activity of the essential oils from i croton adipatus i i croton thurifer i and i croton collinus i |
| topic | antimicrobial antifungal colorimetric microdilution volatile oil medicinal plants gram positive |
| url | https://www.mdpi.com/2079-6382/10/11/1387 |
| work_keys_str_mv | AT juanalizlesliecuchomedrano chemicalprofileofthevolatileconstituentsandantimicrobialactivityoftheessentialoilsfromicrotonadipatusiicrotonthuriferiandicrotoncollinusi AT sammywesleymendozabeingolea chemicalprofileofthevolatileconstituentsandantimicrobialactivityoftheessentialoilsfromicrotonadipatusiicrotonthuriferiandicrotoncollinusi AT cesarmaximofuertesruiton chemicalprofileofthevolatileconstituentsandantimicrobialactivityoftheessentialoilsfromicrotonadipatusiicrotonthuriferiandicrotoncollinusi AT mariaelenasalazarsalvatierra chemicalprofileofthevolatileconstituentsandantimicrobialactivityoftheessentialoilsfromicrotonadipatusiicrotonthuriferiandicrotoncollinusi AT oscarherreracalderon chemicalprofileofthevolatileconstituentsandantimicrobialactivityoftheessentialoilsfromicrotonadipatusiicrotonthuriferiandicrotoncollinusi |