Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis

Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis

Application of human induced pluripotent stem cells (hiPSCs) for tissue regeneration is hindered by off-target cell differentiation. Here, the authors use bulk and single cell RNA-sequencing to identify WNT and MITF as off-target hubs during chondrogenic differentiation; inhibiting these pathways en...

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Bibliographic Details
Main Authors: Chia-Lung Wu, Amanda Dicks, Nancy Steward, Ruhang Tang, Dakota B. Katz, Yun-Rak Choi, Farshid Guilak
Format: Article
Language:English
Published: Nature Portfolio 2021-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20598-y
Description
Summary:Application of human induced pluripotent stem cells (hiPSCs) for tissue regeneration is hindered by off-target cell differentiation. Here, the authors use bulk and single cell RNA-sequencing to identify WNT and MITF as off-target hubs during chondrogenic differentiation; inhibiting these pathways enhanced homogeneity and yield.
ISSN:2041-1723

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