Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis
Application of human induced pluripotent stem cells (hiPSCs) for tissue regeneration is hindered by off-target cell differentiation. Here, the authors use bulk and single cell RNA-sequencing to identify WNT and MITF as off-target hubs during chondrogenic differentiation; inhibiting these pathways en...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20598-y |
| _version_ | 1818716688758079488 |
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| author | Chia-Lung Wu Amanda Dicks Nancy Steward Ruhang Tang Dakota B. Katz Yun-Rak Choi Farshid Guilak |
| author_facet | Chia-Lung Wu Amanda Dicks Nancy Steward Ruhang Tang Dakota B. Katz Yun-Rak Choi Farshid Guilak |
| author_sort | Chia-Lung Wu |
| collection | DOAJ |
| description | Application of human induced pluripotent stem cells (hiPSCs) for tissue regeneration is hindered by off-target cell differentiation. Here, the authors use bulk and single cell RNA-sequencing to identify WNT and MITF as off-target hubs during chondrogenic differentiation; inhibiting these pathways enhanced homogeneity and yield. |
| first_indexed | 2024-12-17T19:23:14Z |
| format | Article |
| id | doaj.art-4749da5248df4c6c9aa0688e0fb689a9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T19:23:14Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-4749da5248df4c6c9aa0688e0fb689a92022-12-21T21:35:26ZengNature PortfolioNature Communications2041-17232021-01-0112111810.1038/s41467-020-20598-ySingle cell transcriptomic analysis of human pluripotent stem cell chondrogenesisChia-Lung Wu0Amanda Dicks1Nancy Steward2Ruhang Tang3Dakota B. Katz4Yun-Rak Choi5Farshid Guilak6Dept. of Orthopaedic Surgery, Washington University in Saint LouisDept. of Orthopaedic Surgery, Washington University in Saint LouisDept. of Orthopaedic Surgery, Washington University in Saint LouisDept. of Orthopaedic Surgery, Washington University in Saint LouisDept. of Orthopaedic Surgery, Washington University in Saint LouisDept. of Orthopaedic Surgery, Washington University in Saint LouisDept. of Orthopaedic Surgery, Washington University in Saint LouisApplication of human induced pluripotent stem cells (hiPSCs) for tissue regeneration is hindered by off-target cell differentiation. Here, the authors use bulk and single cell RNA-sequencing to identify WNT and MITF as off-target hubs during chondrogenic differentiation; inhibiting these pathways enhanced homogeneity and yield.https://doi.org/10.1038/s41467-020-20598-y |
| spellingShingle | Chia-Lung Wu Amanda Dicks Nancy Steward Ruhang Tang Dakota B. Katz Yun-Rak Choi Farshid Guilak Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis Nature Communications |
| title | Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis |
| title_full | Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis |
| title_fullStr | Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis |
| title_full_unstemmed | Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis |
| title_short | Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis |
| title_sort | single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis |
| url | https://doi.org/10.1038/s41467-020-20598-y |
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