The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile
Plant roots navigate in the soil environment following the gravity vector. Cell divisions in the meristem and rapid cell growth in the elongation zone propel the root tips through the soil. Actively elongating cells acidify their apoplast to enable cell wall extension by the activity of plasma membr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2023-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/85193 |
| _version_ | 1797745528982732800 |
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| author | Nelson BC Serre Daša Wernerová Pruthvi Vittal Shiv Mani Dubey Eva Medvecká Adriana Jelínková Jan Petrášek Guido Grossmann Matyáš Fendrych |
| author_facet | Nelson BC Serre Daša Wernerová Pruthvi Vittal Shiv Mani Dubey Eva Medvecká Adriana Jelínková Jan Petrášek Guido Grossmann Matyáš Fendrych |
| author_sort | Nelson BC Serre |
| collection | DOAJ |
| description | Plant roots navigate in the soil environment following the gravity vector. Cell divisions in the meristem and rapid cell growth in the elongation zone propel the root tips through the soil. Actively elongating cells acidify their apoplast to enable cell wall extension by the activity of plasma membrane AHA H+-ATPases. The phytohormone auxin, central regulator of gravitropic response and root development, inhibits root cell growth, likely by rising the pH of the apoplast. However, the role of auxin in the regulation of the apoplastic pH gradient along the root tip is unclear. Here, we show, by using an improved method for visualization and quantification of root surface pH, that the Arabidopsis thaliana root surface pH shows distinct acidic and alkaline zones, which are not primarily determined by the activity of AHA H+-ATPases. Instead, the distinct domain of alkaline pH in the root transition zone is controlled by a rapid auxin response module, consisting of the AUX1 auxin influx carrier, the AFB1 auxin co-receptor, and the CNCG14 calcium channel. We demonstrate that the rapid auxin response pathway is required for an efficient navigation of the root tip. |
| first_indexed | 2024-03-12T15:24:36Z |
| format | Article |
| id | doaj.art-474e1f5d439e4596a6a1335b730c8379 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-12T15:24:36Z |
| publishDate | 2023-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-474e1f5d439e4596a6a1335b730c83792023-08-10T14:44:16ZengeLife Sciences Publications LtdeLife2050-084X2023-07-011210.7554/eLife.85193The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profileNelson BC Serre0Daša Wernerová1Pruthvi Vittal2Shiv Mani Dubey3Eva Medvecká4Adriana Jelínková5Jan Petrášek6Guido Grossmann7https://orcid.org/0000-0001-7529-9244Matyáš Fendrych8https://orcid.org/0000-0002-9767-8699Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech RepublicDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic; Institute of Cell and Interaction Biology, Heinrich-Heine-University Düsseldorf, Düsseldorf, GermanyDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech RepublicDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech RepublicDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech RepublicInstitute of Experimental Botany, Czech Academy of Sciences, Prague, Czech RepublicDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic; Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech RepublicInstitute of Cell and Interaction Biology, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany; CEPLAS - Cluster of Excellence on Plant Sciences, Heinrich-Heine-University Düsseldorf, Düsseldorf, GermanyDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech RepublicPlant roots navigate in the soil environment following the gravity vector. Cell divisions in the meristem and rapid cell growth in the elongation zone propel the root tips through the soil. Actively elongating cells acidify their apoplast to enable cell wall extension by the activity of plasma membrane AHA H+-ATPases. The phytohormone auxin, central regulator of gravitropic response and root development, inhibits root cell growth, likely by rising the pH of the apoplast. However, the role of auxin in the regulation of the apoplastic pH gradient along the root tip is unclear. Here, we show, by using an improved method for visualization and quantification of root surface pH, that the Arabidopsis thaliana root surface pH shows distinct acidic and alkaline zones, which are not primarily determined by the activity of AHA H+-ATPases. Instead, the distinct domain of alkaline pH in the root transition zone is controlled by a rapid auxin response module, consisting of the AUX1 auxin influx carrier, the AFB1 auxin co-receptor, and the CNCG14 calcium channel. We demonstrate that the rapid auxin response pathway is required for an efficient navigation of the root tip.https://elifesciences.org/articles/85193auxinrootpH |
| spellingShingle | Nelson BC Serre Daša Wernerová Pruthvi Vittal Shiv Mani Dubey Eva Medvecká Adriana Jelínková Jan Petrášek Guido Grossmann Matyáš Fendrych The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile eLife auxin root pH |
| title | The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile |
| title_full | The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile |
| title_fullStr | The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile |
| title_full_unstemmed | The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile |
| title_short | The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile |
| title_sort | aux1 afb1 cngc14 module establishes a longitudinal root surface ph profile |
| topic | auxin root pH |
| url | https://elifesciences.org/articles/85193 |
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