Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study
Background. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study,...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2019-12-01
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| Series: | Journal of Dental Research, Dental Clinics, Dental Prospects |
| Subjects: | |
| Online Access: | https://joddd.tbzmed.ac.ir/PDF/joddd-24182 |
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| author | Fereydoon Sargolzaei-Aval Eshagh Ali Saberi Mohammad Reza Arab Narjes Sargolzaei Tayebeh Sanchooli Sima Tavakolinezhad |
| author_facet | Fereydoon Sargolzaei-Aval Eshagh Ali Saberi Mohammad Reza Arab Narjes Sargolzaei Tayebeh Sanchooli Sima Tavakolinezhad |
| author_sort | Fereydoon Sargolzaei-Aval |
| collection | DOAJ |
| description | Background. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study, 36 male Sprague-Dawley rats were used. The animals were randomly assigned to the following experimental groups: OCP (n=12), OCP/Gel (n=12), and the control group (n=12). Defects were created in the rat mandibles and filled with 10 mg of OCP and OCP/Gel disks in the experimental groups. In the control group, however, no substance was administered. Samples were taken on days 7, 14, 21 and 56, respectively, after the implantation. Sections (5 µ) were prepared and stained by H&E. The sections were studied, and the volume fraction of newly formed bone was measured by Dunnett's T3 test based on the significance level (P=0.05). Results. In the experimental groups, the new bone formation began from the margin of defects 7‒14 days after the implantation. During the healing process, the newly formed bone healed a larger area of the defects and grew structurally. In the control group, the defects were primarily filled with dense connective tissue, and only a small amount of new bone was formed. The present study showed a statistically significant difference in the volume of newly formed bone between the experimental groups and the control group (P<0.001). Conclusion. OCP/Gel composite can be beneficial in the healing process of mandibular bone defects. |
| first_indexed | 2024-12-13T02:56:12Z |
| format | Article |
| id | doaj.art-47517c0c88454a1795052a0864b82b0c |
| institution | Directory Open Access Journal |
| issn | 2008-210X 2008-2118 |
| language | English |
| last_indexed | 2024-12-13T02:56:12Z |
| publishDate | 2019-12-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Dental Research, Dental Clinics, Dental Prospects |
| spelling | doaj.art-47517c0c88454a1795052a0864b82b0c2022-12-22T00:01:57ZengTabriz University of Medical SciencesJournal of Dental Research, Dental Clinics, Dental Prospects2008-210X2008-21182019-12-0113425826610.15171/joddd.2019.040joddd-24182Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative studyFereydoon Sargolzaei-Aval0Eshagh Ali Saberi1Mohammad Reza Arab2Narjes Sargolzaei3Tayebeh Sanchooli4Sima Tavakolinezhad5Cellular and Molecular Research Center and Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IranDepartment of Endodontics, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, IranCellular and Molecular Research Center and Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IranDepartment of Community Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IranCellular and Molecular Research Center and Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IranCellular and Molecular Research Center and Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IranBackground. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study, 36 male Sprague-Dawley rats were used. The animals were randomly assigned to the following experimental groups: OCP (n=12), OCP/Gel (n=12), and the control group (n=12). Defects were created in the rat mandibles and filled with 10 mg of OCP and OCP/Gel disks in the experimental groups. In the control group, however, no substance was administered. Samples were taken on days 7, 14, 21 and 56, respectively, after the implantation. Sections (5 µ) were prepared and stained by H&E. The sections were studied, and the volume fraction of newly formed bone was measured by Dunnett's T3 test based on the significance level (P=0.05). Results. In the experimental groups, the new bone formation began from the margin of defects 7‒14 days after the implantation. During the healing process, the newly formed bone healed a larger area of the defects and grew structurally. In the control group, the defects were primarily filled with dense connective tissue, and only a small amount of new bone was formed. The present study showed a statistically significant difference in the volume of newly formed bone between the experimental groups and the control group (P<0.001). Conclusion. OCP/Gel composite can be beneficial in the healing process of mandibular bone defects.https://joddd.tbzmed.ac.ir/PDF/joddd-24182dental enamelfluorideshardness |
| spellingShingle | Fereydoon Sargolzaei-Aval Eshagh Ali Saberi Mohammad Reza Arab Narjes Sargolzaei Tayebeh Sanchooli Sima Tavakolinezhad Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study Journal of Dental Research, Dental Clinics, Dental Prospects dental enamel fluorides hardness |
| title | Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study |
| title_full | Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study |
| title_fullStr | Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study |
| title_full_unstemmed | Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study |
| title_short | Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study |
| title_sort | octacalcium phosphate gelatin composite facilitates bone regeneration of critical sized mandibular defects in rats a quantitative study |
| topic | dental enamel fluorides hardness |
| url | https://joddd.tbzmed.ac.ir/PDF/joddd-24182 |
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