Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide
Objective: To isolate and purify polysaccharides from Dendrobium officinale, characterize their structure and explore their anti-inflammatory effects. Methods: Polysaccharides from Dendrobium officinale were obtained by water extraction and alcohol precipitation. DOP-1 was purified by DEAE-52 cellul...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2024-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023080137 |
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| author | Xiaoliang LIN Lingna XIE Mengxiang CHEN Ming LIANG Yiheng LIANG Zhiyun DU |
| author_facet | Xiaoliang LIN Lingna XIE Mengxiang CHEN Ming LIANG Yiheng LIANG Zhiyun DU |
| author_sort | Xiaoliang LIN |
| collection | DOAJ |
| description | Objective: To isolate and purify polysaccharides from Dendrobium officinale, characterize their structure and explore their anti-inflammatory effects. Methods: Polysaccharides from Dendrobium officinale were obtained by water extraction and alcohol precipitation. DOP-1 was purified by DEAE-52 cellulose ion exchange resin and Sephadex G-100. Ultraviolet scanning, GC, high-performance gel chromatography, infrared spectroscopy and nuclear magnetic resonance scanning were used to analyze the structure of DOP-1 polysaccharide, and the inhibitory effect of DOP-1 on the proliferation of RAW 264.7 cells was determined. And its effect on the expression of NO, TNF-α and IL-1β in RAW264.7 cells induced by LPS. Results: DOP-1 was a homogeneous polysaccharide composed of rhamnose, mannose, glucose and galactose with a monosaccharide molar ratio of 0.6:9.0:2.7:2.9 and a molecular weight of 59.2 kDa. DOP-1 did not significantly inhibit the proliferation of RAW 264.7 cells. Compared with the model group, DOP-1 significantly inhibited the expression of NO, TNF-α and IL-1β. Conclusions: The polysaccharides of Dendrobium officinaleobtained in this study had clear structure and good anti-inflammatory activity, and the extraction conditions were feasible. This study would provide theoretical basis for the development and utilization of polysaccharides of Dendrobium officinale with anti-inflammatory effect. |
| first_indexed | 2024-04-24T05:56:31Z |
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| id | doaj.art-47556b8f53ed4d19b69a98e869301b2a |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-24T05:56:31Z |
| publishDate | 2024-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-47556b8f53ed4d19b69a98e869301b2a2024-04-23T08:59:17ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-04-01458394610.13386/j.issn1002-0306.20230801372023080137-8Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral PolysaccharideXiaoliang LIN0Lingna XIE1Mengxiang CHEN2Ming LIANG3Yiheng LIANG4Zhiyun DU5Infinity (China) Co., Ltd., Guangzhou 510623, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaInfinity (China) Co., Ltd., Guangzhou 510623, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, ChinaObjective: To isolate and purify polysaccharides from Dendrobium officinale, characterize their structure and explore their anti-inflammatory effects. Methods: Polysaccharides from Dendrobium officinale were obtained by water extraction and alcohol precipitation. DOP-1 was purified by DEAE-52 cellulose ion exchange resin and Sephadex G-100. Ultraviolet scanning, GC, high-performance gel chromatography, infrared spectroscopy and nuclear magnetic resonance scanning were used to analyze the structure of DOP-1 polysaccharide, and the inhibitory effect of DOP-1 on the proliferation of RAW 264.7 cells was determined. And its effect on the expression of NO, TNF-α and IL-1β in RAW264.7 cells induced by LPS. Results: DOP-1 was a homogeneous polysaccharide composed of rhamnose, mannose, glucose and galactose with a monosaccharide molar ratio of 0.6:9.0:2.7:2.9 and a molecular weight of 59.2 kDa. DOP-1 did not significantly inhibit the proliferation of RAW 264.7 cells. Compared with the model group, DOP-1 significantly inhibited the expression of NO, TNF-α and IL-1β. Conclusions: The polysaccharides of Dendrobium officinaleobtained in this study had clear structure and good anti-inflammatory activity, and the extraction conditions were feasible. This study would provide theoretical basis for the development and utilization of polysaccharides of Dendrobium officinale with anti-inflammatory effect.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023080137dendrobium officinaleneutral polysaccharideisolation and purificationstructural analysisanti-inflammatory effect |
| spellingShingle | Xiaoliang LIN Lingna XIE Mengxiang CHEN Ming LIANG Yiheng LIANG Zhiyun DU Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide Shipin gongye ke-ji dendrobium officinale neutral polysaccharide isolation and purification structural analysis anti-inflammatory effect |
| title | Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide |
| title_full | Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide |
| title_fullStr | Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide |
| title_full_unstemmed | Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide |
| title_short | Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide |
| title_sort | isolation and purification of polysaccharide and anti inflammatory effect of dendrobium officinale neutral polysaccharide |
| topic | dendrobium officinale neutral polysaccharide isolation and purification structural analysis anti-inflammatory effect |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023080137 |
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