Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure
White Spot Syndrome Virus (WSSV) is one of the main threats to farming <i>Litopenaeus vannamei</i>, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/7/805 |
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| author | Camilla A. Santos Sónia C. S. Andrade Jorge M. O. Fernandes Patrícia D. Freitas |
| author_facet | Camilla A. Santos Sónia C. S. Andrade Jorge M. O. Fernandes Patrícia D. Freitas |
| author_sort | Camilla A. Santos |
| collection | DOAJ |
| description | White Spot Syndrome Virus (WSSV) is one of the main threats to farming <i>Litopenaeus vannamei</i>, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhealthy) <i>L. vannamei,</i> previously exposed to the virus, to obtain new insights about the molecular basis of resistance to WSSV. We detected 71% of our reads mapped against the recently described <i>L. vannamei</i> genome. This is the first report mapping RNA-seq transcripts from shrimps exposed to WSSV against the species reference genome. Differentially expressed gene (DEG) analyses were performed for four independent comparisons, and 13,338 DEGs were identified. When the redundancies and isoforms were disregarded, we observed 8351 and 6514 DEGs, respectively. Interestingly, after crossing the data, we detected a common set of DEGs for hepatopancreas and healthy shrimps, as well as another one for muscle and unhealthy shrimps. Our findings indicate that genes related to apoptosis, melanization, and the Imd pathway are likely to be involved in response to WSSV, offering knowledge about WSSV defense in shrimps exposed to the virus but not infected. These data present potential to be applied in further genetic studies in penaeids and other farmed shrimp species. |
| first_indexed | 2024-03-10T18:26:34Z |
| format | Article |
| id | doaj.art-475678d1a8034f3faed99e5b4886648a |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T18:26:34Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-475678d1a8034f3faed99e5b4886648a2023-11-20T06:55:50ZengMDPI AGGenes2073-44252020-07-0111780510.3390/genes11070805Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) ExposureCamilla A. Santos0Sónia C. S. Andrade1Jorge M. O. Fernandes2Patrícia D. Freitas3Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos 676, BrazilDepartamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, BrazilFaculty of Biosciences and Aquaculture, Nord University, 8026 Bodø, NorwayDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos 676, BrazilWhite Spot Syndrome Virus (WSSV) is one of the main threats to farming <i>Litopenaeus vannamei</i>, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhealthy) <i>L. vannamei,</i> previously exposed to the virus, to obtain new insights about the molecular basis of resistance to WSSV. We detected 71% of our reads mapped against the recently described <i>L. vannamei</i> genome. This is the first report mapping RNA-seq transcripts from shrimps exposed to WSSV against the species reference genome. Differentially expressed gene (DEG) analyses were performed for four independent comparisons, and 13,338 DEGs were identified. When the redundancies and isoforms were disregarded, we observed 8351 and 6514 DEGs, respectively. Interestingly, after crossing the data, we detected a common set of DEGs for hepatopancreas and healthy shrimps, as well as another one for muscle and unhealthy shrimps. Our findings indicate that genes related to apoptosis, melanization, and the Imd pathway are likely to be involved in response to WSSV, offering knowledge about WSSV defense in shrimps exposed to the virus but not infected. These data present potential to be applied in further genetic studies in penaeids and other farmed shrimp species.https://www.mdpi.com/2073-4425/11/7/805innate immunityRNA-seqdifferential expressionWSSV resistanceshrimp |
| spellingShingle | Camilla A. Santos Sónia C. S. Andrade Jorge M. O. Fernandes Patrícia D. Freitas Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure Genes innate immunity RNA-seq differential expression WSSV resistance shrimp |
| title | Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure |
| title_full | Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure |
| title_fullStr | Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure |
| title_full_unstemmed | Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure |
| title_short | Shedding the Light on <i>Litopenaeus vannamei</i> Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure |
| title_sort | shedding the light on i litopenaeus vannamei i differential muscle and hepatopancreas immune responses in white spot syndrome virus wssv exposure |
| topic | innate immunity RNA-seq differential expression WSSV resistance shrimp |
| url | https://www.mdpi.com/2073-4425/11/7/805 |
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