Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>)
Pathogenic infection of fishes is an important constraining factor affecting marine aquaculture. Insufficient understanding of the molecular mechanisms has affected the diagnosis and corresponding treatment. Here, we reported the dynamic changes of gene expression patterns in the Chinese tongue sole...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/9/1144 |
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| author | Xianghui Zhang Xiancai Hao Wenxiu Ma Tengfei Zhu Zhihua Zhang Qian Wang Kaiqiang Liu Changwei Shao Hong-Yan Wang |
| author_facet | Xianghui Zhang Xiancai Hao Wenxiu Ma Tengfei Zhu Zhihua Zhang Qian Wang Kaiqiang Liu Changwei Shao Hong-Yan Wang |
| author_sort | Xianghui Zhang |
| collection | DOAJ |
| description | Pathogenic infection of fishes is an important constraining factor affecting marine aquaculture. Insufficient understanding of the molecular mechanisms has affected the diagnosis and corresponding treatment. Here, we reported the dynamic changes of gene expression patterns in the Chinese tongue sole kidney at 16 h, 48 h, 72 h and 96 h after <i>Vibrio harveyi</i> infection. In total, 366, 214, 115 and 238 differentially expressed genes were obtained from the 16 h−vs. −C, 48 h−vs. −C, 72 h−vs. −C and 96 h−vs. −C group comparisons, respectively. KEGG enrichment analysis revealed rapid up-regulation of several immune-related pathways, including IL-17, TNF and TLR signaling pathway. More importantly, time-series analyses of transcriptome showed that immune genes were specifically up-regulated in a short period of time and then decreased. The expression levels of chemokines increased after infection and reached a peak at 16 h. Specifically, Jak-STAT signaling pathway played a crucial role in the regulation during <i>Vibrio harveyi</i> infection. In the later stages of infection, genes in the neuroendocrine pathway, such as glucocorticoid-related genes, were activated in the kidney, indicating a close connection between the immune system and neuroendocrine system. Our dynamic transcriptome analyses provided profound insight into the gene expression profile and investigation of immunogenetic mechanisms of Chinese tongue sole. |
| first_indexed | 2024-03-10T04:23:45Z |
| format | Article |
| id | doaj.art-4756a3f5e7f94d4ba85a5426c0ef6a24 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T04:23:45Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4756a3f5e7f94d4ba85a5426c0ef6a242023-11-23T07:42:59ZengMDPI AGAnimals2076-26152022-04-01129114410.3390/ani12091144Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>)Xianghui Zhang0Xiancai Hao1Wenxiu Ma2Tengfei Zhu3Zhihua Zhang4Qian Wang5Kaiqiang Liu6Changwei Shao7Hong-Yan Wang8College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266072, ChinaPathogenic infection of fishes is an important constraining factor affecting marine aquaculture. Insufficient understanding of the molecular mechanisms has affected the diagnosis and corresponding treatment. Here, we reported the dynamic changes of gene expression patterns in the Chinese tongue sole kidney at 16 h, 48 h, 72 h and 96 h after <i>Vibrio harveyi</i> infection. In total, 366, 214, 115 and 238 differentially expressed genes were obtained from the 16 h−vs. −C, 48 h−vs. −C, 72 h−vs. −C and 96 h−vs. −C group comparisons, respectively. KEGG enrichment analysis revealed rapid up-regulation of several immune-related pathways, including IL-17, TNF and TLR signaling pathway. More importantly, time-series analyses of transcriptome showed that immune genes were specifically up-regulated in a short period of time and then decreased. The expression levels of chemokines increased after infection and reached a peak at 16 h. Specifically, Jak-STAT signaling pathway played a crucial role in the regulation during <i>Vibrio harveyi</i> infection. In the later stages of infection, genes in the neuroendocrine pathway, such as glucocorticoid-related genes, were activated in the kidney, indicating a close connection between the immune system and neuroendocrine system. Our dynamic transcriptome analyses provided profound insight into the gene expression profile and investigation of immunogenetic mechanisms of Chinese tongue sole.https://www.mdpi.com/2076-2615/12/9/1144Chinese tongue sole<i>Vibrio harveyi</i>immune responsetranscriptome |
| spellingShingle | Xianghui Zhang Xiancai Hao Wenxiu Ma Tengfei Zhu Zhihua Zhang Qian Wang Kaiqiang Liu Changwei Shao Hong-Yan Wang Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) Animals Chinese tongue sole <i>Vibrio harveyi</i> immune response transcriptome |
| title | Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_full | Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_fullStr | Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_full_unstemmed | Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_short | Transcriptome Analysis Indicates Immune Responses against <i>Vibrio harveyi</i> in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) |
| title_sort | transcriptome analysis indicates immune responses against i vibrio harveyi i in chinese tongue sole i cynoglossus semilaevis i |
| topic | Chinese tongue sole <i>Vibrio harveyi</i> immune response transcriptome |
| url | https://www.mdpi.com/2076-2615/12/9/1144 |
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