Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliae
In plant pathogenic fungi, different signalling pathways operate to control responses to nutrient availability during plant infection. A candidate from the cAMP-PKA signalling pathway, the cAMP-dependent protein kinase A gene, pkaC1, and the β-1,6-endoglucanase gene, vegB, involved in cell wall degr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Firenze University Press
2020-01-01
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| Series: | Phytopathologia Mediterranea |
| Subjects: | |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/10917 |
| _version_ | 1818758750914215936 |
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| author | Lugard EBOIGBE Aliki K. TZIMA Epaminondas J. PAPLOMATAS Milton A. TYPAS |
| author_facet | Lugard EBOIGBE Aliki K. TZIMA Epaminondas J. PAPLOMATAS Milton A. TYPAS |
| author_sort | Lugard EBOIGBE |
| collection | DOAJ |
| description | In plant pathogenic fungi, different signalling pathways operate to control responses to nutrient availability during plant infection. A candidate from the cAMP-PKA signalling pathway, the cAMP-dependent protein kinase A gene, pkaC1, and the β-1,6-endoglucanase gene, vegB, involved in cell wall degradation, were studied in V. dahliae. Double mutants of the fungus were constructed, with insertional inactivation in the pkaC1 and vegB genes. Different developmental traits and virulence towards eggplant were evaluated in single and double disruption mutants. In all media tested, double mutants showed better radial growth but less conidia and microsclerotia than the wild type. An interaction between vegB and pkaC1 in controlling virulence on eggplants was recorded, as double mutants were slightly less virulent than the single mutant vegB- , but more virulent than the single mutant pkaC1-. Concomitant or independent function of the two genes and the signaling pathways they operate in for the different growth parameters and virulence are discussed. |
| first_indexed | 2024-12-18T06:31:48Z |
| format | Article |
| id | doaj.art-4758cb2ec20a4f3e8d43f1b239c5497b |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-12-18T06:31:48Z |
| publishDate | 2020-01-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-4758cb2ec20a4f3e8d43f1b239c5497b2022-12-21T21:17:54ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952020-01-0158310.14601/Phyto-10917Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliaeLugard EBOIGBE0Aliki K. TZIMA1Epaminondas J. PAPLOMATAS2Milton A. TYPAS3Division of Genetics and Biotechnology, Department of Biology, National and Kapodistrian University Athens, Panepistemiopolis, Kouponia,15701 Athens, GreeceLaboratory of Plant Pathology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceLaboratory of Plant Pathology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceDivision of Genetics and Biotechnology, Department of Biology, National and Kapodistrian University Athens, Panepistemiopolis, Kouponia,15701 Athens, GreeceIn plant pathogenic fungi, different signalling pathways operate to control responses to nutrient availability during plant infection. A candidate from the cAMP-PKA signalling pathway, the cAMP-dependent protein kinase A gene, pkaC1, and the β-1,6-endoglucanase gene, vegB, involved in cell wall degradation, were studied in V. dahliae. Double mutants of the fungus were constructed, with insertional inactivation in the pkaC1 and vegB genes. Different developmental traits and virulence towards eggplant were evaluated in single and double disruption mutants. In all media tested, double mutants showed better radial growth but less conidia and microsclerotia than the wild type. An interaction between vegB and pkaC1 in controlling virulence on eggplants was recorded, as double mutants were slightly less virulent than the single mutant vegB- , but more virulent than the single mutant pkaC1-. Concomitant or independent function of the two genes and the signaling pathways they operate in for the different growth parameters and virulence are discussed.https://oajournals.fupress.net/index.php/pm/article/view/10917Protein kinase AcAMP-mediated PKAcell wall degrading enzyme |
| spellingShingle | Lugard EBOIGBE Aliki K. TZIMA Epaminondas J. PAPLOMATAS Milton A. TYPAS Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliae Phytopathologia Mediterranea Protein kinase A cAMP-mediated PKA cell wall degrading enzyme |
| title | Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliae |
| title_full | Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliae |
| title_fullStr | Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliae |
| title_full_unstemmed | Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliae |
| title_short | Crosstalk between the cAMP-PKA pathway and the β-1,6-endoglucanase in Verticillium dahliae |
| title_sort | crosstalk between the camp pka pathway and the β 1 6 endoglucanase in verticillium dahliae |
| topic | Protein kinase A cAMP-mediated PKA cell wall degrading enzyme |
| url | https://oajournals.fupress.net/index.php/pm/article/view/10917 |
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