Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology
ABSTRACTThe primary objective of this study was to optimize the production of exopolysaccharide (EPS) produced by L. kefiranofaciens ZW3 through the utilization of response surface methodology (RSM). Central Composite Design (CCD) was used along with three variables to study the response variable. T...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-09-01
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| Series: | International Journal of Food Properties |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2023.2245577 |
| _version_ | 1797202454119448576 |
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| author | Zaheer Ahmed Tahir Mehmood Ifra Ferheen Abdul Wasim Noori Majid Almansouri Muhammad Waseem |
| author_facet | Zaheer Ahmed Tahir Mehmood Ifra Ferheen Abdul Wasim Noori Majid Almansouri Muhammad Waseem |
| author_sort | Zaheer Ahmed |
| collection | DOAJ |
| description | ABSTRACTThe primary objective of this study was to optimize the production of exopolysaccharide (EPS) produced by L. kefiranofaciens ZW3 through the utilization of response surface methodology (RSM). Central Composite Design (CCD) was used along with three variables to study the response variable. The effects of lactose (0.16–1.84%), tryptophan (0.16–0.84%), and pH (5.06–7.24) on EPS production were studied. RSM analysis revealed that coefficient of determination (0.9907) was good for the second-order polynomial model. The optimum values of explanatory variables were 0.36% lactose, 0.23% tryptophan, and pH value of 5.19. Additional experiments were performed to confirm the adequacy of this model. The predicted value of EPS at optimized level was 1609 mg/L, while its experimental value at optimized level was 1602 ± 3 mg/L. This study could help to produce higher amount of EPS from L. kefiranofaciens ZW3, which has a lot of industrial applications. |
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| format | Article |
| id | doaj.art-4758eecb281d4a6dbac1b10df93b9923 |
| institution | Directory Open Access Journal |
| issn | 1094-2912 1532-2386 |
| language | English |
| last_indexed | 2024-04-24T08:03:41Z |
| publishDate | 2023-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj.art-4758eecb281d4a6dbac1b10df93b99232024-04-17T13:20:13ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862023-09-012612285229310.1080/10942912.2023.2245577Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface MethodologyZaheer Ahmed0Tahir Mehmood1Ifra Ferheen2Abdul Wasim Noori3Majid Almansouri4Muhammad Waseem5Department of Nutritional Sciences and Environmental Design, Allama Iqbal Open University (AIOU), Islamabad, PakistanDepartment of Food Science and Technology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, PakistanSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, ItalyDepartment of Food Engineering, Faculty of Chemical industrial Engineering, Kabul Polytechnic University, Kabul, AfghanistanClinical Biochemistry Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Food Science and Technology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, PakistanABSTRACTThe primary objective of this study was to optimize the production of exopolysaccharide (EPS) produced by L. kefiranofaciens ZW3 through the utilization of response surface methodology (RSM). Central Composite Design (CCD) was used along with three variables to study the response variable. The effects of lactose (0.16–1.84%), tryptophan (0.16–0.84%), and pH (5.06–7.24) on EPS production were studied. RSM analysis revealed that coefficient of determination (0.9907) was good for the second-order polynomial model. The optimum values of explanatory variables were 0.36% lactose, 0.23% tryptophan, and pH value of 5.19. Additional experiments were performed to confirm the adequacy of this model. The predicted value of EPS at optimized level was 1609 mg/L, while its experimental value at optimized level was 1602 ± 3 mg/L. This study could help to produce higher amount of EPS from L. kefiranofaciens ZW3, which has a lot of industrial applications.https://www.tandfonline.com/doi/10.1080/10942912.2023.2245577ZW3 EPSResponse surface methodologyLactoseTryptophanpH |
| spellingShingle | Zaheer Ahmed Tahir Mehmood Ifra Ferheen Abdul Wasim Noori Majid Almansouri Muhammad Waseem Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology International Journal of Food Properties ZW3 EPS Response surface methodology Lactose Tryptophan pH |
| title | Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology |
| title_full | Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology |
| title_fullStr | Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology |
| title_full_unstemmed | Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology |
| title_short | Optimization of exopolysaccharide produced by L. kefiranofaciens ZW3 using response surface Methodology |
| title_sort | optimization of exopolysaccharide produced by l kefiranofaciens zw3 using response surface methodology |
| topic | ZW3 EPS Response surface methodology Lactose Tryptophan pH |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2023.2245577 |
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