A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture
Abstract Decellularized xenogeneic vascular grafts can be used in revascularization surgeries. We have developed decellularization methods using high hydrostatic pressure (HHP), which preserves the extracellular structure. Here, we attempted ex vivo endothelialization of HHP-decellularized xenogenei...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26792-w |
| _version_ | 1797977396535623680 |
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| author | Wen-Jin Ho Mako Kobayashi Kozue Murata Yoshihide Hashimoto Kenji Izumi Tsuyoshi Kimura Hideo Kanemitsu Kazuhiro Yamazaki Tadashi Ikeda Kenji Minatoya Akio Kishida Hidetoshi Masumoto |
| author_facet | Wen-Jin Ho Mako Kobayashi Kozue Murata Yoshihide Hashimoto Kenji Izumi Tsuyoshi Kimura Hideo Kanemitsu Kazuhiro Yamazaki Tadashi Ikeda Kenji Minatoya Akio Kishida Hidetoshi Masumoto |
| author_sort | Wen-Jin Ho |
| collection | DOAJ |
| description | Abstract Decellularized xenogeneic vascular grafts can be used in revascularization surgeries. We have developed decellularization methods using high hydrostatic pressure (HHP), which preserves the extracellular structure. Here, we attempted ex vivo endothelialization of HHP-decellularized xenogeneic tissues using human endothelial cells (ECs) to prevent clot formation against human blood. Slices of porcine aortic endothelium were decellularized using HHP and coated with gelatin. Human umbilical vein ECs were directly seeded and cultured under dynamic flow or static conditions for 14 days. Dynamic flow cultures tend to demonstrate higher cell coverage. We then coated the tissues with the E8 fragment of human laminin-411 (hL411), which has high affinity for ECs, and found that Dynamic/hL411showed high area coverage, almost reaching 100% (Dynamic/Gelatin vs Dynamic/hL411; 58.7 ± 11.4 vs 97.5 ± 1.9%, P = 0.0017). Immunostaining revealed sufficient endothelial cell coverage as a single cell layer in Dynamic/hL411. A clot formation assay using human whole blood showed low clot formation in Dynamic/hL411, almost similar to that in the negative control, polytetrafluoroethylene. Surface modification of HHP-decellularized xenogeneic endothelial tissues combined with dynamic culture achieved sufficient ex vivo endothelialization along with prevention of clot formation, indicating their potential for clinical use as vascular grafts in the future. |
| first_indexed | 2024-04-11T05:07:17Z |
| format | Article |
| id | doaj.art-476094af4fbd473280f38c88bf3d1db4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:07:17Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-476094af4fbd473280f38c88bf3d1db42022-12-25T12:17:12ZengNature PortfolioScientific Reports2045-23222022-12-011211910.1038/s41598-022-26792-wA novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic cultureWen-Jin Ho0Mako Kobayashi1Kozue Murata2Yoshihide Hashimoto3Kenji Izumi4Tsuyoshi Kimura5Hideo Kanemitsu6Kazuhiro Yamazaki7Tadashi Ikeda8Kenji Minatoya9Akio Kishida10Hidetoshi Masumoto11Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityDepartment of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityDepartment of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityTokai Hit., Co, Ltd.Department of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityDepartment of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityDepartment of Cardiovascular Surgery, Graduate School of Medicine, Kyoto UniversityAbstract Decellularized xenogeneic vascular grafts can be used in revascularization surgeries. We have developed decellularization methods using high hydrostatic pressure (HHP), which preserves the extracellular structure. Here, we attempted ex vivo endothelialization of HHP-decellularized xenogeneic tissues using human endothelial cells (ECs) to prevent clot formation against human blood. Slices of porcine aortic endothelium were decellularized using HHP and coated with gelatin. Human umbilical vein ECs were directly seeded and cultured under dynamic flow or static conditions for 14 days. Dynamic flow cultures tend to demonstrate higher cell coverage. We then coated the tissues with the E8 fragment of human laminin-411 (hL411), which has high affinity for ECs, and found that Dynamic/hL411showed high area coverage, almost reaching 100% (Dynamic/Gelatin vs Dynamic/hL411; 58.7 ± 11.4 vs 97.5 ± 1.9%, P = 0.0017). Immunostaining revealed sufficient endothelial cell coverage as a single cell layer in Dynamic/hL411. A clot formation assay using human whole blood showed low clot formation in Dynamic/hL411, almost similar to that in the negative control, polytetrafluoroethylene. Surface modification of HHP-decellularized xenogeneic endothelial tissues combined with dynamic culture achieved sufficient ex vivo endothelialization along with prevention of clot formation, indicating their potential for clinical use as vascular grafts in the future.https://doi.org/10.1038/s41598-022-26792-w |
| spellingShingle | Wen-Jin Ho Mako Kobayashi Kozue Murata Yoshihide Hashimoto Kenji Izumi Tsuyoshi Kimura Hideo Kanemitsu Kazuhiro Yamazaki Tadashi Ikeda Kenji Minatoya Akio Kishida Hidetoshi Masumoto A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture Scientific Reports |
| title | A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture |
| title_full | A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture |
| title_fullStr | A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture |
| title_full_unstemmed | A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture |
| title_short | A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture |
| title_sort | novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture |
| url | https://doi.org/10.1038/s41598-022-26792-w |
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