Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development
<p>Abstract</p> <p>Background</p> <p>The Arabidopsis <it>SEUSS </it>(<it>SEU</it>) gene encodes a transcriptional adaptor protein that is required for a diverse set of developmental events, including floral organ identity specification, as well a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-09-01
|
| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/198 |
| _version_ | 1811266348913786880 |
|---|---|
| author | Nole-Wilson Staci Rueschhoff Elizabeth E Bhatti Huda Franks Robert G |
| author_facet | Nole-Wilson Staci Rueschhoff Elizabeth E Bhatti Huda Franks Robert G |
| author_sort | Nole-Wilson Staci |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The Arabidopsis <it>SEUSS </it>(<it>SEU</it>) gene encodes a transcriptional adaptor protein that is required for a diverse set of developmental events, including floral organ identity specification, as well as gynoecium, ovule and embryo development. In order to better understand the molecular mechanisms of <it>SEUSS </it>action we undertook a genetic modifier screen to identify <it>seuss-modifier </it>(<it>sum</it>) mutations.</p> <p>Results</p> <p>Screening of M2 lines representing approximately 5,000 M1 individuals identified mutations that enhance the <it>seuss </it>mutant phenotypic disruptions in ovules and gynoecia; here we describe the phenotype of the <it>sum63 </it>mutant and enhanced disruptions of ovule and gynoecial development in the <it>seu sum63 </it>double mutant. Mapping and genetic complementation tests indicate that <it>sum63 </it>is allelic to <it>CYP85A2 </it>(AT3G30180) a cytochrome p450 enzyme that catalyzes the final steps in the synthesis of the phytohormone brassinolide.</p> <p>Conclusions</p> <p>Our identification of mutations in <it>CYP85A2 </it>as enhancers of the <it>seuss </it>mutant phenotype suggests a previously unrecognized role for brassinolide synthesis in gynoecial and ovule outer integument development. The work also suggests that <it>seuss </it>mutants may be more sensitive to the loss or reduction of brassinolide synthesis than are wild type plants.</p> |
| first_indexed | 2024-04-12T20:40:21Z |
| format | Article |
| id | doaj.art-476154b437fb4269a358aa2165212db7 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T20:40:21Z |
| publishDate | 2010-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-476154b437fb4269a358aa2165212db72022-12-22T03:17:25ZengBMCBMC Plant Biology1471-22292010-09-0110119810.1186/1471-2229-10-198Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule developmentNole-Wilson StaciRueschhoff Elizabeth EBhatti HudaFranks Robert G<p>Abstract</p> <p>Background</p> <p>The Arabidopsis <it>SEUSS </it>(<it>SEU</it>) gene encodes a transcriptional adaptor protein that is required for a diverse set of developmental events, including floral organ identity specification, as well as gynoecium, ovule and embryo development. In order to better understand the molecular mechanisms of <it>SEUSS </it>action we undertook a genetic modifier screen to identify <it>seuss-modifier </it>(<it>sum</it>) mutations.</p> <p>Results</p> <p>Screening of M2 lines representing approximately 5,000 M1 individuals identified mutations that enhance the <it>seuss </it>mutant phenotypic disruptions in ovules and gynoecia; here we describe the phenotype of the <it>sum63 </it>mutant and enhanced disruptions of ovule and gynoecial development in the <it>seu sum63 </it>double mutant. Mapping and genetic complementation tests indicate that <it>sum63 </it>is allelic to <it>CYP85A2 </it>(AT3G30180) a cytochrome p450 enzyme that catalyzes the final steps in the synthesis of the phytohormone brassinolide.</p> <p>Conclusions</p> <p>Our identification of mutations in <it>CYP85A2 </it>as enhancers of the <it>seuss </it>mutant phenotype suggests a previously unrecognized role for brassinolide synthesis in gynoecial and ovule outer integument development. The work also suggests that <it>seuss </it>mutants may be more sensitive to the loss or reduction of brassinolide synthesis than are wild type plants.</p>http://www.biomedcentral.com/1471-2229/10/198 |
| spellingShingle | Nole-Wilson Staci Rueschhoff Elizabeth E Bhatti Huda Franks Robert G Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development BMC Plant Biology |
| title | Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development |
| title_full | Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development |
| title_fullStr | Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development |
| title_full_unstemmed | Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development |
| title_short | Synergistic disruptions in <it>seuss cyp85A2 </it>double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development |
| title_sort | synergistic disruptions in it seuss cyp85a2 it double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development |
| url | http://www.biomedcentral.com/1471-2229/10/198 |
| work_keys_str_mv | AT nolewilsonstaci synergisticdisruptionsinitseusscyp85a2itdoublemutantsrevealaroleforbrassinolidesynthesisduringgynoeciumandovuledevelopment AT rueschhoffelizabethe synergisticdisruptionsinitseusscyp85a2itdoublemutantsrevealaroleforbrassinolidesynthesisduringgynoeciumandovuledevelopment AT bhattihuda synergisticdisruptionsinitseusscyp85a2itdoublemutantsrevealaroleforbrassinolidesynthesisduringgynoeciumandovuledevelopment AT franksrobertg synergisticdisruptionsinitseusscyp85a2itdoublemutantsrevealaroleforbrassinolidesynthesisduringgynoeciumandovuledevelopment |