Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing
This study developed a new burn wound dressing based on core-shell nanofibers that co-deliver antibiotic and antioxidant drugs. For this purpose, poly(ethylene oxide) (PEO)-chitosan (CS)/poly(D,L-lactide-co-glycolide) (PLGA) core-shell nanofibers were fabricated through co-axial electrospinning tech...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1352717/full |
| _version_ | 1797238456898813952 |
|---|---|
| author | Mahshid Monavari Razieh Sohrabi Hamidreza Motasadizadeh Mehran Monavari Yousef Fatahi Yousef Fatahi Negin Mousavi Ejarestaghi Miguel Fuentes-Chandia Aldo Leal-Egaña Mohammad Akrami Mohammad Akrami Shahin Homaeigohar |
| author_facet | Mahshid Monavari Razieh Sohrabi Hamidreza Motasadizadeh Mehran Monavari Yousef Fatahi Yousef Fatahi Negin Mousavi Ejarestaghi Miguel Fuentes-Chandia Aldo Leal-Egaña Mohammad Akrami Mohammad Akrami Shahin Homaeigohar |
| author_sort | Mahshid Monavari |
| collection | DOAJ |
| description | This study developed a new burn wound dressing based on core-shell nanofibers that co-deliver antibiotic and antioxidant drugs. For this purpose, poly(ethylene oxide) (PEO)-chitosan (CS)/poly(D,L-lactide-co-glycolide) (PLGA) core-shell nanofibers were fabricated through co-axial electrospinning technique. Antibiotic levofloxacin (LEV) and antioxidant quercetin (QS) were incorporated into the core and shell parts of PEO-CS/PLGA nanofibers, respectively. The drugs could bond to the polymer chains through hydrogen bonding, leading to their steady release for 168 h. An in vitro drug release study showed a burst effect followed by sustained release of LEV and QS from the nanofibers due to the Fickian diffusion. The NIH 3T3 fibroblast cell viability of the drug loaded core-shell nanofibers was comparable to that in the control (tissue culture polystyrene) implying biocompatibility of the nanofibers and their cell supportive role. However, there was no significant difference in cell viability between the drug loaded and drug free core-shell nanofibers. According to in vivo experiments, PEO-CS-LEV/PLGA-QS core-shell nanofibers could accelerate the healing process of a burn wound compared to a sterile gauze. Thanks to the synergistic therapeutic effect of LEV and QS, a significantly higher wound closure rate was recorded for the drug loaded core-shell nanofibrous dressing than the drug free nanofibers and control. Conclusively, PEO-CS-LEV/PLGA-QS core-shell nanofibers were shown to be a promising wound healing material that could drive the healing cascade through local co-delivery of LEV and QS to burn wounds. |
| first_indexed | 2024-04-24T17:35:56Z |
| format | Article |
| id | doaj.art-47653b8c06af403299138b0e1082cfb2 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-24T17:35:56Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-47653b8c06af403299138b0e1082cfb22024-03-28T05:05:52ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-03-011210.3389/fbioe.2024.13527171352717Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healingMahshid Monavari0Razieh Sohrabi1Hamidreza Motasadizadeh2Mehran Monavari3Yousef Fatahi4Yousef Fatahi5Negin Mousavi Ejarestaghi6Miguel Fuentes-Chandia7Aldo Leal-Egaña8Mohammad Akrami9Mohammad Akrami10Shahin Homaeigohar11Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranDepartment of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranDental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, IranSection eScience (S.3), Federal Institute for Materials Research and Testing, Berlin, GermanyNanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranDepartment of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranDepartment of Biology, Skeletal Research Center, Case Western Reserve University, Cleveland, OH, United StatesInstitute for Molecular Systems Engineering and Advanced Materials, Heidelberg University, Heidelberg, GermanyDepartment of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranInstitute of Biomaterials, University of Tehran & Tehran University of Medical Sciences (IBUTUMS), Tehran, Iran0School of Science and Engineering, University of Dundee, Dundee, United KingdomThis study developed a new burn wound dressing based on core-shell nanofibers that co-deliver antibiotic and antioxidant drugs. For this purpose, poly(ethylene oxide) (PEO)-chitosan (CS)/poly(D,L-lactide-co-glycolide) (PLGA) core-shell nanofibers were fabricated through co-axial electrospinning technique. Antibiotic levofloxacin (LEV) and antioxidant quercetin (QS) were incorporated into the core and shell parts of PEO-CS/PLGA nanofibers, respectively. The drugs could bond to the polymer chains through hydrogen bonding, leading to their steady release for 168 h. An in vitro drug release study showed a burst effect followed by sustained release of LEV and QS from the nanofibers due to the Fickian diffusion. The NIH 3T3 fibroblast cell viability of the drug loaded core-shell nanofibers was comparable to that in the control (tissue culture polystyrene) implying biocompatibility of the nanofibers and their cell supportive role. However, there was no significant difference in cell viability between the drug loaded and drug free core-shell nanofibers. According to in vivo experiments, PEO-CS-LEV/PLGA-QS core-shell nanofibers could accelerate the healing process of a burn wound compared to a sterile gauze. Thanks to the synergistic therapeutic effect of LEV and QS, a significantly higher wound closure rate was recorded for the drug loaded core-shell nanofibrous dressing than the drug free nanofibers and control. Conclusively, PEO-CS-LEV/PLGA-QS core-shell nanofibers were shown to be a promising wound healing material that could drive the healing cascade through local co-delivery of LEV and QS to burn wounds.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1352717/fullcore-shell nanofiberco-axial electrospinningdrug deliveryburn woundwound healing |
| spellingShingle | Mahshid Monavari Razieh Sohrabi Hamidreza Motasadizadeh Mehran Monavari Yousef Fatahi Yousef Fatahi Negin Mousavi Ejarestaghi Miguel Fuentes-Chandia Aldo Leal-Egaña Mohammad Akrami Mohammad Akrami Shahin Homaeigohar Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing Frontiers in Bioengineering and Biotechnology core-shell nanofiber co-axial electrospinning drug delivery burn wound wound healing |
| title | Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing |
| title_full | Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing |
| title_fullStr | Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing |
| title_full_unstemmed | Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing |
| title_short | Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing |
| title_sort | levofloxacin loaded poly ethylene oxide chitosan quercetin loaded poly d l lactide co glycolide core shell electrospun nanofibers for burn wound healing |
| topic | core-shell nanofiber co-axial electrospinning drug delivery burn wound wound healing |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1352717/full |
| work_keys_str_mv | AT mahshidmonavari levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT raziehsohrabi levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT hamidrezamotasadizadeh levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT mehranmonavari levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT youseffatahi levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT youseffatahi levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT neginmousaviejarestaghi levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT miguelfuenteschandia levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT aldolealegana levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT mohammadakrami levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT mohammadakrami levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing AT shahinhomaeigohar levofloxacinloadedpolyethyleneoxidechitosanquercetinloadedpolydllactidecoglycolidecoreshellelectrospunnanofibersforburnwoundhealing |