Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis
AbstractGastric cancer (GC) is a malignant tumour with high lethality. Accruing evidence elucidates the critical adjusting role of long non-coding RNA (lncRNAs) in human cancers. DDX11 antisense RNA 1 (DDX11-AS1) was previously found to be involved in GC pathogenesis. However, the precise molecular...
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| Format: | Article |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1726937 |
| _version_ | 1797788796873342976 |
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| author | Zheng Ren Xiaochun Liu Yaoran Si Desheng Yang |
| author_facet | Zheng Ren Xiaochun Liu Yaoran Si Desheng Yang |
| author_sort | Zheng Ren |
| collection | DOAJ |
| description | AbstractGastric cancer (GC) is a malignant tumour with high lethality. Accruing evidence elucidates the critical adjusting role of long non-coding RNA (lncRNAs) in human cancers. DDX11 antisense RNA 1 (DDX11-AS1) was previously found to be involved in GC pathogenesis. However, the precise molecular mechanisms of DDX11-AS1 need to be further investigated. In this study, we found that DDX11-AS1 expression was up-regulated in GC tumour tissues and cells. Increased DDX11-AS1 expression was associated with advanced TNM stage and lymph node metastasis. Functionally, knockdown of DDX11-AS1 repressed cell proliferation and clone formation, while induced cell cycle arrest and apoptosis. As expected, DDX11-AS1 overexpression displayed the opposite effect. Mechanically, DDX11-AS1 enhanced SPC18 expression through acting as a ceRNA for miR-873-5p. Furthermore, the inhibitory effect of DDX11-AS1 silencing on malignant biological behaviour of GC cells was attenuated by either miR-873-5p inhibitor or SEC11A up-regulation. Moreover, suppression of DDX11-AS1 also decreased GC tumorigenesis in vivo. In conclusion, DDX11-AS1 may serve as an oncogene in GC progression by sponging miR-873-5p and promoting SPC18 expression, providing a new insight into the mechanisms of DDX11-AS1 and elucidating a promising therapy target in GC. |
| first_indexed | 2024-03-13T01:40:35Z |
| format | Article |
| id | doaj.art-4765670a5f284152b418c728c6139593 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-13T01:40:35Z |
| publishDate | 2020-01-01 |
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| spelling | doaj.art-4765670a5f284152b418c728c61395932023-07-03T14:04:56ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148157258310.1080/21691401.2020.1726937Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axisZheng Ren0Xiaochun Liu1Yaoran Si2Desheng Yang3Department of Digestive Diseases, Henan University Huaihe Hospital, Kaifeng, ChinaDepartment of Respiratory Medicine, Henan University Huaihe Hospital, Kaifeng, ChinaDepartment of Digestive Diseases, Henan University Huaihe Hospital, Kaifeng, ChinaDepartment of Digestive Diseases, Henan University Huaihe Hospital, Kaifeng, ChinaAbstractGastric cancer (GC) is a malignant tumour with high lethality. Accruing evidence elucidates the critical adjusting role of long non-coding RNA (lncRNAs) in human cancers. DDX11 antisense RNA 1 (DDX11-AS1) was previously found to be involved in GC pathogenesis. However, the precise molecular mechanisms of DDX11-AS1 need to be further investigated. In this study, we found that DDX11-AS1 expression was up-regulated in GC tumour tissues and cells. Increased DDX11-AS1 expression was associated with advanced TNM stage and lymph node metastasis. Functionally, knockdown of DDX11-AS1 repressed cell proliferation and clone formation, while induced cell cycle arrest and apoptosis. As expected, DDX11-AS1 overexpression displayed the opposite effect. Mechanically, DDX11-AS1 enhanced SPC18 expression through acting as a ceRNA for miR-873-5p. Furthermore, the inhibitory effect of DDX11-AS1 silencing on malignant biological behaviour of GC cells was attenuated by either miR-873-5p inhibitor or SEC11A up-regulation. Moreover, suppression of DDX11-AS1 also decreased GC tumorigenesis in vivo. In conclusion, DDX11-AS1 may serve as an oncogene in GC progression by sponging miR-873-5p and promoting SPC18 expression, providing a new insight into the mechanisms of DDX11-AS1 and elucidating a promising therapy target in GC.https://www.tandfonline.com/doi/10.1080/21691401.2020.1726937DDX11-AS1miR-873-5pSEC11A/SPC18gastric cancer |
| spellingShingle | Zheng Ren Xiaochun Liu Yaoran Si Desheng Yang Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis Artificial Cells, Nanomedicine, and Biotechnology DDX11-AS1 miR-873-5p SEC11A/SPC18 gastric cancer |
| title | Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis |
| title_full | Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis |
| title_fullStr | Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis |
| title_full_unstemmed | Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis |
| title_short | Long non-coding RNA DDX11-AS1 facilitates gastric cancer progression by regulating miR-873-5p/SPC18 axis |
| title_sort | long non coding rna ddx11 as1 facilitates gastric cancer progression by regulating mir 873 5p spc18 axis |
| topic | DDX11-AS1 miR-873-5p SEC11A/SPC18 gastric cancer |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1726937 |
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