The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice
The aim of this study was to determine the effect of voluntary wheel running (VWR) during weight-loss on hepatic lipid and inflammatory biomarkers using a murine model. To induce obesity, male C57Bl/6 mice were fed a 60% high-fat diet (HF) for 10 weeks. At 10 weeks, weight-loss was promoted by rando...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Research in Physiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665944122000037 |
| _version_ | 1811188506674855936 |
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| author | Joshua S. Wooten Kaylee E. Poole Matthew P. Harris Brianne L. Guilford Megan L. Schaller David Umbaugh Andrew Seija |
| author_facet | Joshua S. Wooten Kaylee E. Poole Matthew P. Harris Brianne L. Guilford Megan L. Schaller David Umbaugh Andrew Seija |
| author_sort | Joshua S. Wooten |
| collection | DOAJ |
| description | The aim of this study was to determine the effect of voluntary wheel running (VWR) during weight-loss on hepatic lipid and inflammatory biomarkers using a murine model. To induce obesity, male C57Bl/6 mice were fed a 60% high-fat diet (HF) for 10 weeks. At 10 weeks, weight-loss was promoted by randomizing HF-fed mice to a normal diet (ND) either with (WL + VWR) or without (WL) access to running wheels for 8 weeks. Age-matched dietary control mice were fed either a ND or HF for 18 weeks. Following weight-loss, WL + VWR had a lower body mass compared to all groups despite an average weekly caloric consumption comparable to HF mice. WL + VWR had an increased adiponectin concentration when compared to WL, but no difference between WL and WL + VWR was observed for plasma glucose and lipid biomarkers. When compared to HF, the lower hepatic total lipids in both WL and WL + VWR were associated with increased pAMPK:AMPK and reduced pACC-1:ACC-1 ratios. When compared to WL, WL + VWR resulted in lower hepatic cholesterol and trended to lower hepatic triglyceride. In both WL and WL + VWR, pNF-κB p65:NF-κB p65 ratio was lower than HF and comparable to ND. TGFβ1 and BAMBI protein levels were evaluated as biomarkers for hepatic fibrosis. No differences in TGFβ1 was observed between groups; however, WL and WL + VWR had BAMBI protein levels comparable to ND. Overall, the addition of voluntary exercise resulted in greater weight-loss and improvements in hepatic cholesterol and triglyceride levels; however, limited improvements in hepatic inflammation were observed when compared to weight-loss by diet alone. |
| first_indexed | 2024-04-11T14:21:02Z |
| format | Article |
| id | doaj.art-476be5a11cf44e6182d0b8b8e3ffd9a8 |
| institution | Directory Open Access Journal |
| issn | 2665-9441 |
| language | English |
| last_indexed | 2024-04-11T14:21:02Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Physiology |
| spelling | doaj.art-476be5a11cf44e6182d0b8b8e3ffd9a82022-12-22T04:19:04ZengElsevierCurrent Research in Physiology2665-94412022-01-0156372The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J miceJoshua S. Wooten0Kaylee E. Poole1Matthew P. Harris2Brianne L. Guilford3Megan L. Schaller4David Umbaugh5Andrew Seija6Corresponding author. Department of Applied Health Southern Illinois University Edwardsville, Campus Box 1126, Edwardsville, IL, 62026-1126, USA; Department of Applied Health, Southern Illinois University Edwardsville, Edwardsville, IL, USADepartment of Applied Health, Southern Illinois University Edwardsville, Edwardsville, IL, USADepartment of Applied Health, Southern Illinois University Edwardsville, Edwardsville, IL, USADepartment of Applied Health, Southern Illinois University Edwardsville, Edwardsville, IL, USADepartment of Applied Health, Southern Illinois University Edwardsville, Edwardsville, IL, USADepartment of Applied Health, Southern Illinois University Edwardsville, Edwardsville, IL, USADepartment of Applied Health, Southern Illinois University Edwardsville, Edwardsville, IL, USAThe aim of this study was to determine the effect of voluntary wheel running (VWR) during weight-loss on hepatic lipid and inflammatory biomarkers using a murine model. To induce obesity, male C57Bl/6 mice were fed a 60% high-fat diet (HF) for 10 weeks. At 10 weeks, weight-loss was promoted by randomizing HF-fed mice to a normal diet (ND) either with (WL + VWR) or without (WL) access to running wheels for 8 weeks. Age-matched dietary control mice were fed either a ND or HF for 18 weeks. Following weight-loss, WL + VWR had a lower body mass compared to all groups despite an average weekly caloric consumption comparable to HF mice. WL + VWR had an increased adiponectin concentration when compared to WL, but no difference between WL and WL + VWR was observed for plasma glucose and lipid biomarkers. When compared to HF, the lower hepatic total lipids in both WL and WL + VWR were associated with increased pAMPK:AMPK and reduced pACC-1:ACC-1 ratios. When compared to WL, WL + VWR resulted in lower hepatic cholesterol and trended to lower hepatic triglyceride. In both WL and WL + VWR, pNF-κB p65:NF-κB p65 ratio was lower than HF and comparable to ND. TGFβ1 and BAMBI protein levels were evaluated as biomarkers for hepatic fibrosis. No differences in TGFβ1 was observed between groups; however, WL and WL + VWR had BAMBI protein levels comparable to ND. Overall, the addition of voluntary exercise resulted in greater weight-loss and improvements in hepatic cholesterol and triglyceride levels; however, limited improvements in hepatic inflammation were observed when compared to weight-loss by diet alone.http://www.sciencedirect.com/science/article/pii/S2665944122000037LipotoxicityInflammationObesityLipid metabolismMetabolic syndromeExercise |
| spellingShingle | Joshua S. Wooten Kaylee E. Poole Matthew P. Harris Brianne L. Guilford Megan L. Schaller David Umbaugh Andrew Seija The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice Current Research in Physiology Lipotoxicity Inflammation Obesity Lipid metabolism Metabolic syndrome Exercise |
| title | The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice |
| title_full | The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice |
| title_fullStr | The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice |
| title_full_unstemmed | The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice |
| title_short | The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice |
| title_sort | effects of voluntary wheel running during weight loss on biomarkers of hepatic lipid metabolism and inflammation in c57bl 6j mice |
| topic | Lipotoxicity Inflammation Obesity Lipid metabolism Metabolic syndrome Exercise |
| url | http://www.sciencedirect.com/science/article/pii/S2665944122000037 |
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