Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta
Abstract Background A wide range of molecules are used by tapeworm metacestodes to establish successful infection in the hostile environment of the host. Reports indicating the proteins in the cestode-host interactions are limited predominantly to taeniids, with no previous data available for non-ta...
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| Language: | English |
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BMC
2017-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-017-2519-4 |
| _version_ | 1818111856487694336 |
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| author | Anna Sulima Justyna Bień Kirsi Savijoki Anu Näreaho Rusłan Sałamatin David Bruce Conn Daniel Młocicki |
| author_facet | Anna Sulima Justyna Bień Kirsi Savijoki Anu Näreaho Rusłan Sałamatin David Bruce Conn Daniel Młocicki |
| author_sort | Anna Sulima |
| collection | DOAJ |
| description | Abstract Background A wide range of molecules are used by tapeworm metacestodes to establish successful infection in the hostile environment of the host. Reports indicating the proteins in the cestode-host interactions are limited predominantly to taeniids, with no previous data available for non-taeniid species. A non-taeniid, Hymenolepis diminuta, represents one of the most important model species in cestode biology and exhibits an exceptional developmental plasticity in its life-cycle, which involves two phylogenetically distant hosts, arthropod and vertebrate. Results We identified H. diminuta cysticercoid proteins that were recognized by sera of H. diminuta-infected rats using two-dimensional gel electrophoresis (2DE), 2D-immunoblotting, and LC-MS/MS mass spectrometry. Proteomic analysis of 42 antigenic spots revealed 70 proteins. The largest number belonged to structural proteins and to the heat-shock protein (HSP) family. These results show a number of the antigenic proteins of the cysticercoid stage, which were present already in the insect host prior to contact with the mammal host. These are the first parasite antigens that the mammal host encounters after the infection, therefore they may represent some of the molecules important in host-parasite interactions at the early stage of infection. Conclusions These results could help in understanding how H. diminuta and other cestodes adapt to their diverse and complex parasitic life-cycles and show universal molecules used among diverse groups of cestodes to escape the host response to infection. |
| first_indexed | 2024-12-11T03:09:41Z |
| format | Article |
| id | doaj.art-476c5e385b274ed7bb6aa0214c1faf18 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-11T03:09:41Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-476c5e385b274ed7bb6aa0214c1faf182022-12-22T01:22:53ZengBMCParasites & Vectors1756-33052017-11-0110111210.1186/s13071-017-2519-4Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminutaAnna Sulima0Justyna Bień1Kirsi Savijoki2Anu Näreaho3Rusłan Sałamatin4David Bruce Conn5Daniel Młocicki6Department of General Biology and Parasitology, Medical University of WarsawWitold Stefański Institute of Parasitology, Polish Academy of SciencesDepartment of Food and Environmental Sciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of General Biology and Parasitology, Medical University of WarsawOne Health Center, Berry CollegeDepartment of General Biology and Parasitology, Medical University of WarsawAbstract Background A wide range of molecules are used by tapeworm metacestodes to establish successful infection in the hostile environment of the host. Reports indicating the proteins in the cestode-host interactions are limited predominantly to taeniids, with no previous data available for non-taeniid species. A non-taeniid, Hymenolepis diminuta, represents one of the most important model species in cestode biology and exhibits an exceptional developmental plasticity in its life-cycle, which involves two phylogenetically distant hosts, arthropod and vertebrate. Results We identified H. diminuta cysticercoid proteins that were recognized by sera of H. diminuta-infected rats using two-dimensional gel electrophoresis (2DE), 2D-immunoblotting, and LC-MS/MS mass spectrometry. Proteomic analysis of 42 antigenic spots revealed 70 proteins. The largest number belonged to structural proteins and to the heat-shock protein (HSP) family. These results show a number of the antigenic proteins of the cysticercoid stage, which were present already in the insect host prior to contact with the mammal host. These are the first parasite antigens that the mammal host encounters after the infection, therefore they may represent some of the molecules important in host-parasite interactions at the early stage of infection. Conclusions These results could help in understanding how H. diminuta and other cestodes adapt to their diverse and complex parasitic life-cycles and show universal molecules used among diverse groups of cestodes to escape the host response to infection.http://link.springer.com/article/10.1186/s13071-017-2519-4Hymenolepis diminutaImmunogenic proteinsMass spectrometryTwo-dimensional electrophoresis |
| spellingShingle | Anna Sulima Justyna Bień Kirsi Savijoki Anu Näreaho Rusłan Sałamatin David Bruce Conn Daniel Młocicki Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta Parasites & Vectors Hymenolepis diminuta Immunogenic proteins Mass spectrometry Two-dimensional electrophoresis |
| title | Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta |
| title_full | Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta |
| title_fullStr | Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta |
| title_full_unstemmed | Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta |
| title_short | Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta |
| title_sort | identification of immunogenic proteins of the cysticercoid of hymenolepis diminuta |
| topic | Hymenolepis diminuta Immunogenic proteins Mass spectrometry Two-dimensional electrophoresis |
| url | http://link.springer.com/article/10.1186/s13071-017-2519-4 |
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