Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other Services
Introduction: The prevalence and spread rate of the Escherichia coli ST131 clone continue to be an important issue requiring investigation. This study investigated the presence of the ST131 clone in E. coli isolates isolated from intensive care units (ICUs) and other services using real-time polymer...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Turkish |
| Published: |
Galenos Yayinevi
2023-06-01
|
| Series: | Mediterranean Journal of Infection, Microbes and Antimicrobials |
| Subjects: | |
| Online Access: | https://mjima.org/text.php?&id=368 |
| _version_ | 1797809705901359104 |
|---|---|
| author | Figen HASLİ Kemal BİLGİN Hamza KADI Yeliz TANRIVERDİ ÇAYCI Asuman BİRİNCİ |
| author_facet | Figen HASLİ Kemal BİLGİN Hamza KADI Yeliz TANRIVERDİ ÇAYCI Asuman BİRİNCİ |
| author_sort | Figen HASLİ |
| collection | DOAJ |
| description | Introduction: The prevalence and spread rate of the Escherichia coli ST131 clone continue to be an important issue requiring investigation. This study investigated the presence of the ST131 clone in E. coli isolates isolated from intensive care units (ICUs) and other services using real-time polymerase chain reaction (PCR).
Materials and Methods: A total of 200 E. coli isolates were included in the study. The double-disk synergy method was used to show the presence of extended-spectrum beta-lactamases (ESBL). The ST131 clones were investigated using the real-time PCR method in all isolates.
Results: E. coli ST131 clones were identified in 17 of 100 strains isolated from the specimens from ICUs. Of these strains, 15 (29.4%) of the 51 ESBL positive isolates and two (4.1%) of the 49 ESBL negative isolates were identified as E. coli ST131 clones. E. coli ST131 clones were identified in 13 of 100 strains isolated from non-ICUs. Among these strains, 12 (34.3%) of 35 ESBL positive isolates and one (1.5%) of the 65 ESBL negative isolates were identified as E. coli ST131 clones.
Conclusion: While the E. coli ST131 clone was common in ESBL-producing isolates, its presence did not differ in intensive or non-ICUs. |
| first_indexed | 2024-03-13T06:56:45Z |
| format | Article |
| id | doaj.art-476c79ea83034a5f9e9cf02f1337b72f |
| institution | Directory Open Access Journal |
| issn | 2147-673X |
| language | Turkish |
| last_indexed | 2024-03-13T06:56:45Z |
| publishDate | 2023-06-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Mediterranean Journal of Infection, Microbes and Antimicrobials |
| spelling | doaj.art-476c79ea83034a5f9e9cf02f1337b72f2023-06-07T05:56:50ZturGalenos YayineviMediterranean Journal of Infection, Microbes and Antimicrobials2147-673X2023-06-0112110.4274/mjima.galenos.2023.2022.8Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other ServicesFigen HASLİ0https://orcid.org/0000-0002-8761-4704Kemal BİLGİN1https://orcid.org/0000-0002-8892-2223Hamza KADI2https://orcid.org/0000-0002-7706-4251Yeliz TANRIVERDİ ÇAYCI3https://orcid.org/0000-0002-9251-1953Asuman BİRİNCİ4https://orcid.org/0000-0002-8653-4710Ondokuz Mayıs University Faculty of Medicine, Department of Medical Microbiology, Samsun, TurkeyOndokuz Mayıs University Faculty of Medicine, Department of Medical Microbiology, Samsun, TurkeyVeterinary Control Institute, Department of Virology, Samsun, TurkeyOndokuz Mayıs University Faculty of Medicine, Department of Medical Microbiology, Samsun, TurkeyOndokuz Mayıs University Faculty of Medicine, Department of Medical Microbiology, Samsun, TurkeyIntroduction: The prevalence and spread rate of the Escherichia coli ST131 clone continue to be an important issue requiring investigation. This study investigated the presence of the ST131 clone in E. coli isolates isolated from intensive care units (ICUs) and other services using real-time polymerase chain reaction (PCR). Materials and Methods: A total of 200 E. coli isolates were included in the study. The double-disk synergy method was used to show the presence of extended-spectrum beta-lactamases (ESBL). The ST131 clones were investigated using the real-time PCR method in all isolates. Results: E. coli ST131 clones were identified in 17 of 100 strains isolated from the specimens from ICUs. Of these strains, 15 (29.4%) of the 51 ESBL positive isolates and two (4.1%) of the 49 ESBL negative isolates were identified as E. coli ST131 clones. E. coli ST131 clones were identified in 13 of 100 strains isolated from non-ICUs. Among these strains, 12 (34.3%) of 35 ESBL positive isolates and one (1.5%) of the 65 ESBL negative isolates were identified as E. coli ST131 clones. Conclusion: While the E. coli ST131 clone was common in ESBL-producing isolates, its presence did not differ in intensive or non-ICUs.https://mjima.org/text.php?&id=368e. colist131intensive care unit |
| spellingShingle | Figen HASLİ Kemal BİLGİN Hamza KADI Yeliz TANRIVERDİ ÇAYCI Asuman BİRİNCİ Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other Services Mediterranean Journal of Infection, Microbes and Antimicrobials e. coli st131 intensive care unit |
| title | Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other Services |
| title_full | Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other Services |
| title_fullStr | Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other Services |
| title_full_unstemmed | Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other Services |
| title_short | Investigation of the ST131 Clone by Real-time PCR in Escherichia coli Strains Isolated in Patients from Intensive Care Units and Other Services |
| title_sort | investigation of the st131 clone by real time pcr in escherichia coli strains isolated in patients from intensive care units and other services |
| topic | e. coli st131 intensive care unit |
| url | https://mjima.org/text.php?&id=368 |
| work_keys_str_mv | AT figenhasli investigationofthest131clonebyrealtimepcrinescherichiacolistrainsisolatedinpatientsfromintensivecareunitsandotherservices AT kemalbilgin investigationofthest131clonebyrealtimepcrinescherichiacolistrainsisolatedinpatientsfromintensivecareunitsandotherservices AT hamzakadi investigationofthest131clonebyrealtimepcrinescherichiacolistrainsisolatedinpatientsfromintensivecareunitsandotherservices AT yeliztanriverdicayci investigationofthest131clonebyrealtimepcrinescherichiacolistrainsisolatedinpatientsfromintensivecareunitsandotherservices AT asumanbirinci investigationofthest131clonebyrealtimepcrinescherichiacolistrainsisolatedinpatientsfromintensivecareunitsandotherservices |