Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453)
Background: Breast cancer is one of the most common cancers among women. Incorrect pattern of gene expression involved in epigenetic Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer. resveratrol is a natural flavonoids that antioxidant and anti-c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2017-10-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2756-1&slc_lang=en&sid=1 |
| _version_ | 1818522376570142720 |
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| author | Hadis Ahmadirad Mohammad Reza Hajizadeh Hajizadeh Mehdi Mahmoodi Mohamadreza Mirzaee Fahime Mohammadain Shahrbabaki Mostafa Soltaninejad |
| author_facet | Hadis Ahmadirad Mohammad Reza Hajizadeh Hajizadeh Mehdi Mahmoodi Mohamadreza Mirzaee Fahime Mohammadain Shahrbabaki Mostafa Soltaninejad |
| author_sort | Hadis Ahmadirad |
| collection | DOAJ |
| description | Background: Breast cancer is one of the most common cancers among women. Incorrect pattern of gene expression involved in epigenetic Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer.
resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of resveratrol, this study examined effect of resveratrol on the expression of genes referred to the two classes of breast cancer cell lines
Materials and methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of two doses of 25 and 100 micro molar for 24 hours of resveratrol for were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression.
Results: The results showed that resveratrol decrease in gene expression APOBEC3B and DNMT-1 And increased expression of TET_1 in both of cell lines. .
Discussion: due to satisfactory effects of resveratrol has on breast cancer cells, probably these effects through epigenetic mechanisms applied. However, the final decision needs to be further investigation. |
| first_indexed | 2024-12-11T05:32:27Z |
| format | Article |
| id | doaj.art-476ce66bed744ef1a10c5c5e3807d81d |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-11T05:32:27Z |
| publishDate | 2017-10-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-476ce66bed744ef1a10c5c5e3807d81d2022-12-22T01:19:23ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332017-10-01257526536Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453)Hadis Ahmadirad0Mohammad Reza Hajizadeh Hajizadeh1Mehdi Mahmoodi2Mohamadreza Mirzaee3Fahime Mohammadain Shahrbabaki4Mostafa Soltaninejad5 University of Medical Sciences, Rafsanjan, Iran Molecular Medicine Research Center, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Rafsanjan University of Medical Sciences, Rafsanjan, Iran Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran University of Medical Sciences, Rafsanjan, Iran Rafsanjan University of Medical Sciences, Rafsanjan, Iran Background: Breast cancer is one of the most common cancers among women. Incorrect pattern of gene expression involved in epigenetic Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer. resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of resveratrol, this study examined effect of resveratrol on the expression of genes referred to the two classes of breast cancer cell lines Materials and methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of two doses of 25 and 100 micro molar for 24 hours of resveratrol for were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression. Results: The results showed that resveratrol decrease in gene expression APOBEC3B and DNMT-1 And increased expression of TET_1 in both of cell lines. . Discussion: due to satisfactory effects of resveratrol has on breast cancer cells, probably these effects through epigenetic mechanisms applied. However, the final decision needs to be further investigation.http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2756-1&slc_lang=en&sid=1Resveratrol Gene expression Epigenetics Cell lines Breast cancer MCF-7 MDA-MB-453 |
| spellingShingle | Hadis Ahmadirad Mohammad Reza Hajizadeh Hajizadeh Mehdi Mahmoodi Mohamadreza Mirzaee Fahime Mohammadain Shahrbabaki Mostafa Soltaninejad Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453) Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Resveratrol Gene expression Epigenetics Cell lines Breast cancer MCF-7 MDA-MB-453 |
| title | Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453) |
| title_full | Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453) |
| title_fullStr | Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453) |
| title_full_unstemmed | Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453) |
| title_short | Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453) |
| title_sort | effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines mcf 7 mda mb 453 |
| topic | Resveratrol Gene expression Epigenetics Cell lines Breast cancer MCF-7 MDA-MB-453 |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2756-1&slc_lang=en&sid=1 |
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