Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> Lind
Plant pathogens evade basal defense systems and attack different organs and tissues of plants. Genetic engineering of plants with genes that confer resistance against pathogens is very effective in pathogen control. Conventional breeding for disease resistance in ornamental crops is difficult and la...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/9/4735 |
| _version_ | 1797504544744144896 |
|---|---|
| author | Satoru Maeda Katsutomo Sasaki Hisatoshi Kaku Yasukazu Kanda Norihiro Ohtsubo Masaki Mori |
| author_facet | Satoru Maeda Katsutomo Sasaki Hisatoshi Kaku Yasukazu Kanda Norihiro Ohtsubo Masaki Mori |
| author_sort | Satoru Maeda |
| collection | DOAJ |
| description | Plant pathogens evade basal defense systems and attack different organs and tissues of plants. Genetic engineering of plants with genes that confer resistance against pathogens is very effective in pathogen control. Conventional breeding for disease resistance in ornamental crops is difficult and lagging relative to that in non-ornamental crops due to an inadequate number of disease-resistant genes. Therefore, genetic engineering of these plants with defense-conferring genes is a practical approach. We used rice <i>BSR2</i> encoding CYP78A15 for developing transgenic <i>Torenia fournieri</i> Lind. lines. The overexpression of <i>BSR2</i> conferred resistance against two devastating fungal pathogens, <i>Rhizoctonia solani</i> and <i>Botrytis cinerea</i>. In addition, <i>BSR2</i> overexpression resulted in enlarged flowers with enlarged floral organs. Histological observation of the petal cells suggested that the enlargement in the floral organs could be due to the elongation and expansion of the cells. Therefore, the overexpression of <i>BSR2</i> confers broad-spectrum disease resistance and induces the production of enlarged flowers simultaneously. Therefore, this could be an effective strategy for developing ornamental crops that are disease-resistant and economically more valuable. |
| first_indexed | 2024-03-10T04:06:06Z |
| format | Article |
| id | doaj.art-4770649e1e804389a5c993896e3433b6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:06:06Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4770649e1e804389a5c993896e3433b62023-11-23T08:21:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239473510.3390/ijms23094735Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> LindSatoru Maeda0Katsutomo Sasaki1Hisatoshi Kaku2Yasukazu Kanda3Norihiro Ohtsubo4Masaki Mori5Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NIAS), Tsukuba 305-8602, JapanInstitute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization (NIVFS), Tsukuba 305-0852, JapanInstitute of Agrobiological Sciences, National Agriculture and Food Research Organization (NIAS), Tsukuba 305-8602, JapanInstitute of Agrobiological Sciences, National Agriculture and Food Research Organization (NIAS), Tsukuba 305-8602, JapanInstitute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization (NIVFS), Tsukuba 305-0852, JapanInstitute of Agrobiological Sciences, National Agriculture and Food Research Organization (NIAS), Tsukuba 305-8602, JapanPlant pathogens evade basal defense systems and attack different organs and tissues of plants. Genetic engineering of plants with genes that confer resistance against pathogens is very effective in pathogen control. Conventional breeding for disease resistance in ornamental crops is difficult and lagging relative to that in non-ornamental crops due to an inadequate number of disease-resistant genes. Therefore, genetic engineering of these plants with defense-conferring genes is a practical approach. We used rice <i>BSR2</i> encoding CYP78A15 for developing transgenic <i>Torenia fournieri</i> Lind. lines. The overexpression of <i>BSR2</i> conferred resistance against two devastating fungal pathogens, <i>Rhizoctonia solani</i> and <i>Botrytis cinerea</i>. In addition, <i>BSR2</i> overexpression resulted in enlarged flowers with enlarged floral organs. Histological observation of the petal cells suggested that the enlargement in the floral organs could be due to the elongation and expansion of the cells. Therefore, the overexpression of <i>BSR2</i> confers broad-spectrum disease resistance and induces the production of enlarged flowers simultaneously. Therefore, this could be an effective strategy for developing ornamental crops that are disease-resistant and economically more valuable.https://www.mdpi.com/1422-0067/23/9/4735torenia<i>BSR2</i>disease resistancegenetic engineering<i>Botrytis cinerea</i><i>Rhizoctonia solani</i> |
| spellingShingle | Satoru Maeda Katsutomo Sasaki Hisatoshi Kaku Yasukazu Kanda Norihiro Ohtsubo Masaki Mori Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> Lind International Journal of Molecular Sciences torenia <i>BSR2</i> disease resistance genetic engineering <i>Botrytis cinerea</i> <i>Rhizoctonia solani</i> |
| title | Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> Lind |
| title_full | Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> Lind |
| title_fullStr | Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> Lind |
| title_full_unstemmed | Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> Lind |
| title_short | Overexpression of Rice <i>BSR2</i> Confers Disease Resistance and Induces Enlarged Flowers in <i>Torenia fournieri</i> Lind |
| title_sort | overexpression of rice i bsr2 i confers disease resistance and induces enlarged flowers in i torenia fournieri i lind |
| topic | torenia <i>BSR2</i> disease resistance genetic engineering <i>Botrytis cinerea</i> <i>Rhizoctonia solani</i> |
| url | https://www.mdpi.com/1422-0067/23/9/4735 |
| work_keys_str_mv | AT satorumaeda overexpressionofriceibsr2iconfersdiseaseresistanceandinducesenlargedflowersinitoreniafournieriilind AT katsutomosasaki overexpressionofriceibsr2iconfersdiseaseresistanceandinducesenlargedflowersinitoreniafournieriilind AT hisatoshikaku overexpressionofriceibsr2iconfersdiseaseresistanceandinducesenlargedflowersinitoreniafournieriilind AT yasukazukanda overexpressionofriceibsr2iconfersdiseaseresistanceandinducesenlargedflowersinitoreniafournieriilind AT norihiroohtsubo overexpressionofriceibsr2iconfersdiseaseresistanceandinducesenlargedflowersinitoreniafournieriilind AT masakimori overexpressionofriceibsr2iconfersdiseaseresistanceandinducesenlargedflowersinitoreniafournieriilind |