Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines
Cancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/12/2/348 |
| _version_ | 1797709663060361216 |
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| author | Julie Lafontaine Jean-Sébastien Boisvert Audrey Glory Sylvain Coulombe Philip Wong |
| author_facet | Julie Lafontaine Jean-Sébastien Boisvert Audrey Glory Sylvain Coulombe Philip Wong |
| author_sort | Julie Lafontaine |
| collection | DOAJ |
| description | Cancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, and can induce cancer cell death both in vitro and <i>in vivo</i>. Here, we used proliferation assay to characterize the plasma sensitivity of fourteen breast cancer cell lines. These assays showed that all tested cell lines were sensitive to NTP. In addition, a good correlation was found comparing cell sensitivity to NTP and radiation therapy (RT), where cells that were sensitive to RT were also sensitive to plasma. Moreover, in some breast cancer cell lines, NTP and RT have a synergistic effect. Adding a dose of PARP-inhibitor olaparib to NTP treatment always increases the efficacy of the treatment. Olaparib also exhibits a synergistic effect with NTP, especially in triple negative breast cancer cells. Results presented here help elucidate the position of plasma use as a potential breast cancer treatment. |
| first_indexed | 2024-03-12T06:40:58Z |
| format | Article |
| id | doaj.art-4770e6f1dd3c4fefa9de6fa265c0c2dc |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T06:40:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-4770e6f1dd3c4fefa9de6fa265c0c2dc2023-09-03T01:01:38ZengMDPI AGCancers2072-66942020-02-0112234810.3390/cancers12020348cancers12020348Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell LinesJulie Lafontaine0Jean-Sébastien Boisvert1Audrey Glory2Sylvain Coulombe3Philip Wong4Institut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaInstitut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaInstitut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaPlasma Processing Laboratory, Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, QC H3A 0C5, CanadaInstitut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaCancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, and can induce cancer cell death both in vitro and <i>in vivo</i>. Here, we used proliferation assay to characterize the plasma sensitivity of fourteen breast cancer cell lines. These assays showed that all tested cell lines were sensitive to NTP. In addition, a good correlation was found comparing cell sensitivity to NTP and radiation therapy (RT), where cells that were sensitive to RT were also sensitive to plasma. Moreover, in some breast cancer cell lines, NTP and RT have a synergistic effect. Adding a dose of PARP-inhibitor olaparib to NTP treatment always increases the efficacy of the treatment. Olaparib also exhibits a synergistic effect with NTP, especially in triple negative breast cancer cells. Results presented here help elucidate the position of plasma use as a potential breast cancer treatment.https://www.mdpi.com/2072-6694/12/2/348radiation therapynon-thermal plasmaradio-frequency dischargebreast cancerparp-inhibitorolaparibdna-damage |
| spellingShingle | Julie Lafontaine Jean-Sébastien Boisvert Audrey Glory Sylvain Coulombe Philip Wong Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines Cancers radiation therapy non-thermal plasma radio-frequency discharge breast cancer parp-inhibitor olaparib dna-damage |
| title | Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines |
| title_full | Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines |
| title_fullStr | Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines |
| title_full_unstemmed | Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines |
| title_short | Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines |
| title_sort | synergy between non thermal plasma with radiation therapy and olaparib in a panel of breast cancer cell lines |
| topic | radiation therapy non-thermal plasma radio-frequency discharge breast cancer parp-inhibitor olaparib dna-damage |
| url | https://www.mdpi.com/2072-6694/12/2/348 |
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