Translocation of Drought-Responsive Proteins from the Chloroplasts
Some chloroplast proteins are known to serve as messengers to transmit retrograde signals from chloroplasts to the nuclei in response to environmental stresses. However, whether particular chloroplast proteins respond to drought stress and serve as messengers for retrograde signal transduction are u...
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MDPI AG
2020-01-01
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| author | Ping Li Haoju Liu Hong Yang Xiaojun Pu Chuanhong Li Heqiang Huo Zhaohui Chu Yuxiao Chang Yongjun Lin Li Liu |
| author_facet | Ping Li Haoju Liu Hong Yang Xiaojun Pu Chuanhong Li Heqiang Huo Zhaohui Chu Yuxiao Chang Yongjun Lin Li Liu |
| author_sort | Ping Li |
| collection | DOAJ |
| description | Some chloroplast proteins are known to serve as messengers to transmit retrograde signals from chloroplasts to the nuclei in response to environmental stresses. However, whether particular chloroplast proteins respond to drought stress and serve as messengers for retrograde signal transduction are unclear. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) to monitor the proteomic changes in tobacco (<i>Nicotiana benthamiana</i>) treated with drought stress/re-watering. We identified 3936 and 1087 differentially accumulated total leaf and chloroplast proteins, respectively, which were grouped into 16 categories. Among these, one particular category of proteins, that includes carbonic anhydrase 1 (CA1), exhibited a great decline in chloroplasts, but a remarkable increase in leaves under drought stress. The subcellular localizations of CA1 proteins from moss (<i>Physcomitrella patens</i>), <i>Arabidopsis thaliana</i> and rice (<i>Oryza sativa</i>) in <i>P. patens</i> protoplasts consistently showed that CA1 proteins gradually diminished within chloroplasts but increasingly accumulated in the cytosol under osmotic stress treatment, suggesting that they could be translocated from chloroplasts to the cytosol and act as a signal messenger from the chloroplast. Our results thus highlight the potential importance of chloroplast proteins in retrograde signaling pathways and provide a set of candidate proteins for further research. |
| first_indexed | 2024-03-12T06:32:52Z |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:32:52Z |
| publishDate | 2020-01-01 |
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| spelling | doaj.art-4774cc7389e44d81a05c60bb07e17e562023-09-03T01:29:57ZengMDPI AGCells2073-44092020-01-019125910.3390/cells9010259cells9010259Translocation of Drought-Responsive Proteins from the ChloroplastsPing Li0Haoju Liu1Hong Yang2Xiaojun Pu3Chuanhong Li4Heqiang Huo5Zhaohui Chu6Yuxiao Chang7Yongjun Lin8Li Liu9National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory for Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaKey Laboratory for Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaNational Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaMid-Florida Research and Education Center, Department of Environmental Horticulture, University of Florida, Miami, FL 32703, USAState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Taian 271018, ChinaAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaNational Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory for Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaSome chloroplast proteins are known to serve as messengers to transmit retrograde signals from chloroplasts to the nuclei in response to environmental stresses. However, whether particular chloroplast proteins respond to drought stress and serve as messengers for retrograde signal transduction are unclear. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) to monitor the proteomic changes in tobacco (<i>Nicotiana benthamiana</i>) treated with drought stress/re-watering. We identified 3936 and 1087 differentially accumulated total leaf and chloroplast proteins, respectively, which were grouped into 16 categories. Among these, one particular category of proteins, that includes carbonic anhydrase 1 (CA1), exhibited a great decline in chloroplasts, but a remarkable increase in leaves under drought stress. The subcellular localizations of CA1 proteins from moss (<i>Physcomitrella patens</i>), <i>Arabidopsis thaliana</i> and rice (<i>Oryza sativa</i>) in <i>P. patens</i> protoplasts consistently showed that CA1 proteins gradually diminished within chloroplasts but increasingly accumulated in the cytosol under osmotic stress treatment, suggesting that they could be translocated from chloroplasts to the cytosol and act as a signal messenger from the chloroplast. Our results thus highlight the potential importance of chloroplast proteins in retrograde signaling pathways and provide a set of candidate proteins for further research.https://www.mdpi.com/2073-4409/9/1/259chloroplastitraqdroughtproteomicsretrograde signal |
| spellingShingle | Ping Li Haoju Liu Hong Yang Xiaojun Pu Chuanhong Li Heqiang Huo Zhaohui Chu Yuxiao Chang Yongjun Lin Li Liu Translocation of Drought-Responsive Proteins from the Chloroplasts Cells chloroplast itraq drought proteomics retrograde signal |
| title | Translocation of Drought-Responsive Proteins from the Chloroplasts |
| title_full | Translocation of Drought-Responsive Proteins from the Chloroplasts |
| title_fullStr | Translocation of Drought-Responsive Proteins from the Chloroplasts |
| title_full_unstemmed | Translocation of Drought-Responsive Proteins from the Chloroplasts |
| title_short | Translocation of Drought-Responsive Proteins from the Chloroplasts |
| title_sort | translocation of drought responsive proteins from the chloroplasts |
| topic | chloroplast itraq drought proteomics retrograde signal |
| url | https://www.mdpi.com/2073-4409/9/1/259 |
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