Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species
The repertoires of bitter taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We stud...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2016.00055/full |
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| author | Ana M. Ferreira Ana M. Ferreira Andreia T. Marques Luca eFontanesi Carl-Gustaf eThulin Elvira eSales-Baptista Susana S. Araújo Susana S. Araújo André M. Almeida |
| author_facet | Ana M. Ferreira Ana M. Ferreira Andreia T. Marques Luca eFontanesi Carl-Gustaf eThulin Elvira eSales-Baptista Susana S. Araújo Susana S. Araújo André M. Almeida |
| author_sort | Ana M. Ferreira |
| collection | DOAJ |
| description | The repertoires of bitter taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, Lepus europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi and Sylvilagus floridanus, using Oryctolagus cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of Oryctolagus cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in Romerolagus diazi and Sylvilagus floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification. |
| first_indexed | 2024-12-12T14:32:45Z |
| format | Article |
| id | doaj.art-4778ca007a134368977a7bad57a7bd82 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-12T14:32:45Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-4778ca007a134368977a7bad57a7bd822022-12-22T00:21:28ZengFrontiers Media S.A.Frontiers in Genetics1664-80212016-04-01710.3389/fgene.2016.00055148739Identification of a bitter-taste receptor gene repertoire in different Lagomorphs speciesAna M. Ferreira0Ana M. Ferreira1Andreia T. Marques2Luca eFontanesi3Carl-Gustaf eThulin4Elvira eSales-Baptista5Susana S. Araújo6Susana S. Araújo7André M. Almeida8University of ÉvoraInstituto de Tecnologia Química e Biológica António Xavier - Universidade Nova de LisboaUniversità degli Studi di MilanoALMA MATER STUDIORUM - University of BolognaSwedish University of Agricultural SciencesUniversity of ÉvoraInstituto de Tecnologia Química e Biológica António Xavier - Universidade Nova de LisboaUniversità degli Studi di PaviaRoss UniversityThe repertoires of bitter taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, Lepus europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi and Sylvilagus floridanus, using Oryctolagus cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of Oryctolagus cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in Romerolagus diazi and Sylvilagus floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification.http://journal.frontiersin.org/Journal/10.3389/fgene.2016.00055/fullOryctolagusTAS2RlagomorphLepusSylvilagusRomerolagus |
| spellingShingle | Ana M. Ferreira Ana M. Ferreira Andreia T. Marques Luca eFontanesi Carl-Gustaf eThulin Elvira eSales-Baptista Susana S. Araújo Susana S. Araújo André M. Almeida Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species Frontiers in Genetics Oryctolagus TAS2R lagomorph Lepus Sylvilagus Romerolagus |
| title | Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
| title_full | Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
| title_fullStr | Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
| title_full_unstemmed | Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
| title_short | Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
| title_sort | identification of a bitter taste receptor gene repertoire in different lagomorphs species |
| topic | Oryctolagus TAS2R lagomorph Lepus Sylvilagus Romerolagus |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2016.00055/full |
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