PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance.
Leucine-rich repeats receptor-like kinases (LRR-RLKs) play important roles in plant growth and development as well as stress responses. Here, 56 LRR-RLK genes were identified in the Antarctic moss Pohlia nutans transcriptome, which were further classified into 11 subgroups based on their extracellul...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5328275?pdf=render |
| _version_ | 1818310821434884096 |
|---|---|
| author | Jing Wang Shenghao Liu Chengcheng Li Tailin Wang Pengying Zhang Kaoshan Chen |
| author_facet | Jing Wang Shenghao Liu Chengcheng Li Tailin Wang Pengying Zhang Kaoshan Chen |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Leucine-rich repeats receptor-like kinases (LRR-RLKs) play important roles in plant growth and development as well as stress responses. Here, 56 LRR-RLK genes were identified in the Antarctic moss Pohlia nutans transcriptome, which were further classified into 11 subgroups based on their extracellular domain. Of them, PnLRR-RLK27 belongs to the LRR II subgroup and its expression was significantly induced by abiotic stresses. Subcellular localization analysis showed that PnLRR-RLK27 was a plasma membrane protein. The overexpression of PnLRR-RLK27 in Physcomitrella significantly enhanced the salinity and ABA tolerance in their gametophyte growth. Similarly, PnLRR-RLK27 heterologous expression in Arabidopsis increased the salinity and ABA tolerance in their seed germination and early root growth as well as the tolerance to oxidative stress. PnLRR-RLK27 overproduction in these transgenic plants increased the expression of salt stress/ABA-related genes. Furthermore, PnLRR-RLK27 increased the activities of reactive oxygen species (ROS) scavengers and reduced the levels of malondialdehyde (MDA) and ROS. Taken together, these results suggested that PnLRR-RLK27 as a signaling regulator confer abiotic stress response associated with the regulation of the stress- and ABA-mediated signaling network. |
| first_indexed | 2024-12-13T07:52:09Z |
| format | Article |
| id | doaj.art-47826c0f292f434591d84db61cbdc123 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T07:52:09Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-47826c0f292f434591d84db61cbdc1232022-12-21T23:54:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017286910.1371/journal.pone.0172869PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance.Jing WangShenghao LiuChengcheng LiTailin WangPengying ZhangKaoshan ChenLeucine-rich repeats receptor-like kinases (LRR-RLKs) play important roles in plant growth and development as well as stress responses. Here, 56 LRR-RLK genes were identified in the Antarctic moss Pohlia nutans transcriptome, which were further classified into 11 subgroups based on their extracellular domain. Of them, PnLRR-RLK27 belongs to the LRR II subgroup and its expression was significantly induced by abiotic stresses. Subcellular localization analysis showed that PnLRR-RLK27 was a plasma membrane protein. The overexpression of PnLRR-RLK27 in Physcomitrella significantly enhanced the salinity and ABA tolerance in their gametophyte growth. Similarly, PnLRR-RLK27 heterologous expression in Arabidopsis increased the salinity and ABA tolerance in their seed germination and early root growth as well as the tolerance to oxidative stress. PnLRR-RLK27 overproduction in these transgenic plants increased the expression of salt stress/ABA-related genes. Furthermore, PnLRR-RLK27 increased the activities of reactive oxygen species (ROS) scavengers and reduced the levels of malondialdehyde (MDA) and ROS. Taken together, these results suggested that PnLRR-RLK27 as a signaling regulator confer abiotic stress response associated with the regulation of the stress- and ABA-mediated signaling network.http://europepmc.org/articles/PMC5328275?pdf=render |
| spellingShingle | Jing Wang Shenghao Liu Chengcheng Li Tailin Wang Pengying Zhang Kaoshan Chen PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance. PLoS ONE |
| title | PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance. |
| title_full | PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance. |
| title_fullStr | PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance. |
| title_full_unstemmed | PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance. |
| title_short | PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance. |
| title_sort | pnlrr rlk27 a novel leucine rich repeats receptor like protein kinase from the antarctic moss pohlia nutans positively regulates salinity and oxidation stress tolerance |
| url | http://europepmc.org/articles/PMC5328275?pdf=render |
| work_keys_str_mv | AT jingwang pnlrrrlk27anovelleucinerichrepeatsreceptorlikeproteinkinasefromtheantarcticmosspohlianutanspositivelyregulatessalinityandoxidationstresstolerance AT shenghaoliu pnlrrrlk27anovelleucinerichrepeatsreceptorlikeproteinkinasefromtheantarcticmosspohlianutanspositivelyregulatessalinityandoxidationstresstolerance AT chengchengli pnlrrrlk27anovelleucinerichrepeatsreceptorlikeproteinkinasefromtheantarcticmosspohlianutanspositivelyregulatessalinityandoxidationstresstolerance AT tailinwang pnlrrrlk27anovelleucinerichrepeatsreceptorlikeproteinkinasefromtheantarcticmosspohlianutanspositivelyregulatessalinityandoxidationstresstolerance AT pengyingzhang pnlrrrlk27anovelleucinerichrepeatsreceptorlikeproteinkinasefromtheantarcticmosspohlianutanspositivelyregulatessalinityandoxidationstresstolerance AT kaoshanchen pnlrrrlk27anovelleucinerichrepeatsreceptorlikeproteinkinasefromtheantarcticmosspohlianutanspositivelyregulatessalinityandoxidationstresstolerance |