| Summary: | Economic value development of peony is limited by the fuzzy genetic relationships. Phylogeny investigation, genomic resource conservation, and genome evolution could be elucidated by the chloroplast genome (cp) on account of the extremely conservative structure. Paeonia lactiflora ‘Lv He’, a new peony variety blooming with green flowers, possess excellent ornamental value. Molecular structure and phylogenetic analysis of the plastome of Paeonia lactiflora ‘Lv He’ was resolved in this study. The complete cp genome contained a typical quadripartite structure with a length of 152, 702 bp and an overall 38.4% GC content. The circular molecule of the genome was constituted of a large single-copy (LSC) region of 84,373 bp with 36.7% GC content, a small single-copy (SSC) region of 16,969 bp with 32.7% GC content, and a pair of inverted repeat (IRa and IRb) regions of 25,680 bp each with 43.1% GC content. The genome annotated 115 genes, containg 81 protein-coding genes, 30 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes. Phylogenetic analysis revealed a sister taxonomical relationship to P. lactiflora and P. obovate.
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