Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics
Pseudomonas oryzihabitans is uncommon pathogen, but recently there may be warning of this bacterium to be dangerous, highly virulence, and may cause increase in morbidity and mortality rate, especially if there are multi-drug resistance and biofilm former. Biofilm forming bacteria display resistance...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2019-03-01
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| Series: | Nano Biomedicine and Engineering |
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| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v11i1.p11-17 |
| _version_ | 1797673368103682048 |
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| author | Shaimaa Obaid Hasson |
| author_facet | Shaimaa Obaid Hasson |
| author_sort | Shaimaa Obaid Hasson |
| collection | DOAJ |
| description | Pseudomonas oryzihabitans is uncommon pathogen, but recently there may be warning of this bacterium to be dangerous, highly virulence, and may cause increase in morbidity and mortality rate, especially if there are multi-drug resistance and biofilm former. Biofilm forming bacteria display resistance to antibiotics, reaching to 1000 time higher than planktonic bacteria. The aim of the current study was to detect Pseudomonas oryzihabitans as biofilm producer and determine the susceptibility to the tested antibiotics. Pseudomonas oryzihabitans was isolated from urine of catheterized patients in Iraqi hospitals and diagnosed as biofilm former phenotypically by congo red and tissue culture plate methods, and genetically by polymerase chain reaction analysis to detect lasR gene (quarm sensing gene) as biofilm indicator and determine its susceptibility to antibiotics by disc diffusion and VITEK AST method. The results revealed that Pseudomonas oryzihabitans was present at 3 isolates; all of them had lasR gene and were resistant to most tested antibiotics at 100%, except that it showed sensitivity rate reaching to 100% to imipenem. From the present study, it was concluded that Pseudomonas oryzihabitans was isolated for the first time as biofilm producer from urinary catheter which possessed lasR gene as biofilm formation indicator, and the multi-drug resistance was considered as a distinctive feature of biofilm formation bacteria. |
| first_indexed | 2024-03-11T21:43:29Z |
| format | Article |
| id | doaj.art-478a918eaa8f4925adf3a324d2fb2c10 |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2024-03-11T21:43:29Z |
| publishDate | 2019-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-478a918eaa8f4925adf3a324d2fb2c102023-09-26T11:02:56ZengTsinghua University PressNano Biomedicine and Engineering2150-55782019-03-01111111710.5101/nbe.v11i1.p11-17Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to AntibioticsShaimaa Obaid Hasson0Al-Qasim Green University, Veterinary Collage, Department of Microbiology, IraqPseudomonas oryzihabitans is uncommon pathogen, but recently there may be warning of this bacterium to be dangerous, highly virulence, and may cause increase in morbidity and mortality rate, especially if there are multi-drug resistance and biofilm former. Biofilm forming bacteria display resistance to antibiotics, reaching to 1000 time higher than planktonic bacteria. The aim of the current study was to detect Pseudomonas oryzihabitans as biofilm producer and determine the susceptibility to the tested antibiotics. Pseudomonas oryzihabitans was isolated from urine of catheterized patients in Iraqi hospitals and diagnosed as biofilm former phenotypically by congo red and tissue culture plate methods, and genetically by polymerase chain reaction analysis to detect lasR gene (quarm sensing gene) as biofilm indicator and determine its susceptibility to antibiotics by disc diffusion and VITEK AST method. The results revealed that Pseudomonas oryzihabitans was present at 3 isolates; all of them had lasR gene and were resistant to most tested antibiotics at 100%, except that it showed sensitivity rate reaching to 100% to imipenem. From the present study, it was concluded that Pseudomonas oryzihabitans was isolated for the first time as biofilm producer from urinary catheter which possessed lasR gene as biofilm formation indicator, and the multi-drug resistance was considered as a distinctive feature of biofilm formation bacteria.https://www.sciopen.com/article/10.5101/nbe.v11i1.p11-17pseudomonas oryzihabitansbiofilmmulti-drug resistanceantibioticsurinary catheter |
| spellingShingle | Shaimaa Obaid Hasson Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics Nano Biomedicine and Engineering pseudomonas oryzihabitans biofilm multi-drug resistance antibiotics urinary catheter |
| title | Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics |
| title_full | Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics |
| title_fullStr | Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics |
| title_full_unstemmed | Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics |
| title_short | Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics |
| title_sort | phenotypic and genotypic detection of biofilm formation pseudomonas oryzihabitance and susceptibility to antibiotics |
| topic | pseudomonas oryzihabitans biofilm multi-drug resistance antibiotics urinary catheter |
| url | https://www.sciopen.com/article/10.5101/nbe.v11i1.p11-17 |
| work_keys_str_mv | AT shaimaaobaidhasson phenotypicandgenotypicdetectionofbiofilmformationpseudomonasoryzihabitanceandsusceptibilitytoantibiotics |