Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management
Prospecting for potential novel Bacillus thuringiensis with new holotype crystal toxins was carried out in the Western ghats hill range of Karnataka state, India. From the soil samples collected three Bt isolates SBIKWG 12, SBIKWG 24 and SBIKWG 70 were isolated. Of these while the two isolates, name...
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| Format: | Article |
| Language: | English |
| Published: |
Society for Sugarcane Research and Development
2021-08-01
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| Series: | Journal of Sugarcane Research |
| Subjects: | |
| Online Access: | http://localhost/ojs-3.3.0-13/index.php/JSR/article/view/101370 |
| _version_ | 1797957016426119168 |
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| author | B Singaravelu G.S Suresha J Srikanth C Appunu C Sankaranarayanan P Mahesh R Nirmala M Rajeshkumar |
| author_facet | B Singaravelu G.S Suresha J Srikanth C Appunu C Sankaranarayanan P Mahesh R Nirmala M Rajeshkumar |
| author_sort | B Singaravelu |
| collection | DOAJ |
| description | Prospecting for potential novel Bacillus thuringiensis with new holotype crystal toxins was carried out in the Western ghats hill range of Karnataka state, India. From the soil samples collected three Bt isolates SBIKWG 12, SBIKWG 24 and SBIKWG 70 were isolated. Of these while the two isolates, namely SBIKWG 12 and SBIKWG 24 produced bipyramidal crystal toxins, the third isolate produced spherical crystal. PCR screening of the isolates revealed the presence of lepidopteran and coleopteran active cry genes. Partial sequences obtained from these isolates revealed the presence of multiple crystal toxin genes. BlastX analysis of the partial gene sequences indicated the potential for the occurrence of new holotype crystal toxin genes in SBIKWG 24 and SBIKWG 70. |
| first_indexed | 2024-04-10T23:57:42Z |
| format | Article |
| id | doaj.art-4793878f10b649d5b531867d13dde16c |
| institution | Directory Open Access Journal |
| issn | 2249-927X 2582-4767 |
| language | English |
| last_indexed | 2024-04-10T23:57:42Z |
| publishDate | 2021-08-01 |
| publisher | Society for Sugarcane Research and Development |
| record_format | Article |
| series | Journal of Sugarcane Research |
| spelling | doaj.art-4793878f10b649d5b531867d13dde16c2023-01-10T10:03:56ZengSociety for Sugarcane Research and DevelopmentJournal of Sugarcane Research2249-927X2582-47672021-08-01102Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest managementB Singaravelu0G.S Suresha1J Srikanth2C Appunu3C Sankaranarayanan4P Mahesh5R Nirmala6M Rajeshkumar7ICAR-SUGARCANE BREEDING INSTITUTEICAR-SUGARCANE BREEDING INSTITUTEICAR-SUGARCANE BREEDING INSTITUTEICAR-SUGARCANE BREEDING INSTITUTEICAR-SUGARCANE BREEDING INSTITUTEICAR-SUGARCANE BREEDING INSTITUTEICAR-SUGARCANE BREEDING INSTITUTEICAR-SUGARCANE BREEDING INSTITUTEProspecting for potential novel Bacillus thuringiensis with new holotype crystal toxins was carried out in the Western ghats hill range of Karnataka state, India. From the soil samples collected three Bt isolates SBIKWG 12, SBIKWG 24 and SBIKWG 70 were isolated. Of these while the two isolates, namely SBIKWG 12 and SBIKWG 24 produced bipyramidal crystal toxins, the third isolate produced spherical crystal. PCR screening of the isolates revealed the presence of lepidopteran and coleopteran active cry genes. Partial sequences obtained from these isolates revealed the presence of multiple crystal toxin genes. BlastX analysis of the partial gene sequences indicated the potential for the occurrence of new holotype crystal toxin genes in SBIKWG 24 and SBIKWG 70.http://localhost/ojs-3.3.0-13/index.php/JSR/article/view/101370Bacillus thuringiensisBorersColeopteracry1 genecry8 geneHolotypes |
| spellingShingle | B Singaravelu G.S Suresha J Srikanth C Appunu C Sankaranarayanan P Mahesh R Nirmala M Rajeshkumar Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management Journal of Sugarcane Research Bacillus thuringiensis Borers Coleoptera cry1 gene cry8 gene Holotypes |
| title | Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management |
| title_full | Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management |
| title_fullStr | Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management |
| title_full_unstemmed | Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management |
| title_short | Prospecting in Western Ghats of Karnataka for indigenous Bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management |
| title_sort | prospecting in western ghats of karnataka for indigenous bacillus thuringiensis isolates harbouring novel crystal toxin genes for sugarcane pest management |
| topic | Bacillus thuringiensis Borers Coleoptera cry1 gene cry8 gene Holotypes |
| url | http://localhost/ojs-3.3.0-13/index.php/JSR/article/view/101370 |
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