Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure
Aim. To compare the effect of increased concentrations of aggregation inducers (five-fold addition) under standard conditions and under the conditions of vortex flow in vitro on platelet aggregation in patients with chronic heart failure (CHF).Materials and methods. The study included 28 patients. T...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2022-07-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4822 |
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| author | O. A. Trubacheva I. V. Kologrivova T. E. Suslova A. V. Swarovskaya A. A. Garganeeva |
| author_facet | O. A. Trubacheva I. V. Kologrivova T. E. Suslova A. V. Swarovskaya A. A. Garganeeva |
| author_sort | O. A. Trubacheva |
| collection | DOAJ |
| description | Aim. To compare the effect of increased concentrations of aggregation inducers (five-fold addition) under standard conditions and under the conditions of vortex flow in vitro on platelet aggregation in patients with chronic heart failure (CHF).Materials and methods. The study included 28 patients. The activity of platelet aggregation in platelet-rich plasma (PRP) was evaluated according to light transmission curves (%) and the average size of aggregates (relative units (rel. units)). The aggregation inducer was added once at 10 seconds of the study (standard procedure) and five times at 10 seconds, 1, 2, 3, and 4 minutes of the study with a constant stirring rate of 800 rpm. The same parameters were evaluated under the conditions of vortex flow, which was created by changing the stirring rate of the PRP from 800 rpm to 0 rpm and again to 800 rpm by pressing the centrifugation button on the analyzer.Results. In the course of the study, the size of the aggregates increased in patients with CHF only under the conditions of vortex flow. When a collagen aggregation inducer was added both at the concentration of 2 mmol / l and 10 mmol / l, platelet aggregation parameters increased under the conditions of vortex flow. During the study of epinephrine-induced platelet aggregation in patients with CHF, an increase in the aggregation parameters was revealed, both at five-fold addition of the inducer and under the conditions of vortex flow compared with the standard method.Conclusion. The proposed methodological approaches to creating the conditions for vortex flow in vitro and to five-fold addition of epinephrine showed an increase in the size of the aggregates and the degree of platelet aggregation. Collagen-induced aggregation under the conditions of vortex flow revealed 7 (25%) patients with high residual platelet reactivity (HRPR), and epinephrine-induced aggregation detected 15 (54%) patients with HRPR. |
| first_indexed | 2024-04-10T01:05:08Z |
| format | Article |
| id | doaj.art-4799fdf0cd944a679d511285fe90849f |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:05:08Z |
| publishDate | 2022-07-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-4799fdf0cd944a679d511285fe90849f2023-03-13T09:58:28ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842022-07-0121212212810.20538/1682-0363-2022-2-122-1282898Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failureO. A. Trubacheva0I. V. Kologrivova1T. E. Suslova2A. V. Swarovskaya3A. A. Garganeeva4Научно-исследовательский институт (НИИ) кардиологии, Томский национальный исследовательский медицинский центр (НИМЦ) Российской академии наукНаучно-исследовательский институт (НИИ) кардиологии, Томский национальный исследовательский медицинский центр (НИМЦ) Российской академии наукНаучно-исследовательский институт (НИИ) кардиологии, Томский национальный исследовательский медицинский центр (НИМЦ) Российской академии наукНаучно-исследовательский институт (НИИ) кардиологии, Томский национальный исследовательский медицинский центр (НИМЦ) Российской академии наукНаучно-исследовательский институт (НИИ) кардиологии, Томский национальный исследовательский медицинский центр (НИМЦ) Российской академии наукAim. To compare the effect of increased concentrations of aggregation inducers (five-fold addition) under standard conditions and under the conditions of vortex flow in vitro on platelet aggregation in patients with chronic heart failure (CHF).Materials and methods. The study included 28 patients. The activity of platelet aggregation in platelet-rich plasma (PRP) was evaluated according to light transmission curves (%) and the average size of aggregates (relative units (rel. units)). The aggregation inducer was added once at 10 seconds of the study (standard procedure) and five times at 10 seconds, 1, 2, 3, and 4 minutes of the study with a constant stirring rate of 800 rpm. The same parameters were evaluated under the conditions of vortex flow, which was created by changing the stirring rate of the PRP from 800 rpm to 0 rpm and again to 800 rpm by pressing the centrifugation button on the analyzer.Results. In the course of the study, the size of the aggregates increased in patients with CHF only under the conditions of vortex flow. When a collagen aggregation inducer was added both at the concentration of 2 mmol / l and 10 mmol / l, platelet aggregation parameters increased under the conditions of vortex flow. During the study of epinephrine-induced platelet aggregation in patients with CHF, an increase in the aggregation parameters was revealed, both at five-fold addition of the inducer and under the conditions of vortex flow compared with the standard method.Conclusion. The proposed methodological approaches to creating the conditions for vortex flow in vitro and to five-fold addition of epinephrine showed an increase in the size of the aggregates and the degree of platelet aggregation. Collagen-induced aggregation under the conditions of vortex flow revealed 7 (25%) patients with high residual platelet reactivity (HRPR), and epinephrine-induced aggregation detected 15 (54%) patients with HRPR.https://bulletin.ssmu.ru/jour/article/view/4822агрегациятромбоцитколлагенэпинефринхроническая сердечная недостаточностьостаточная реактивность |
| spellingShingle | O. A. Trubacheva I. V. Kologrivova T. E. Suslova A. V. Swarovskaya A. A. Garganeeva Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure Бюллетень сибирской медицины агрегация тромбоцит коллаген эпинефрин хроническая сердечная недостаточность остаточная реактивность |
| title | Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure |
| title_full | Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure |
| title_fullStr | Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure |
| title_full_unstemmed | Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure |
| title_short | Platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure |
| title_sort | platelet aggregation under the conditions of vortex flow in vitro in patients with chronic heart failure |
| topic | агрегация тромбоцит коллаген эпинефрин хроническая сердечная недостаточность остаточная реактивность |
| url | https://bulletin.ssmu.ru/jour/article/view/4822 |
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