Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoes
<p>Abstract</p> <p>As part of a routine screening for the possible presence of the necrotic strains of potato virus Y affecting potatoes in Mexico, five PVY isolates were submitted to biological and molecular analysis. Considering their serological properties, two belong to the com...
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| Format: | Article |
| Language: | English |
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BMC
2009-05-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/6/1/48 |
| _version_ | 1818113545404940288 |
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| author | Martínez-Soriano Juan Silva-Rosales Laura Frías-Treviño Gustavo Aviña-Padilla Katia Ramírez-Rodríguez Victoriano |
| author_facet | Martínez-Soriano Juan Silva-Rosales Laura Frías-Treviño Gustavo Aviña-Padilla Katia Ramírez-Rodríguez Victoriano |
| author_sort | Martínez-Soriano Juan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>As part of a routine screening for the possible presence of the necrotic strains of potato virus Y affecting potatoes in Mexico, five PVY isolates were submitted to biological and molecular analysis. Considering their serological properties, two belong to the common strain (O) and three to the necrotic strain (N). All the isolates induced vein necrosis in <it>Nicotiana tabacum</it>. To characterize the isolates, 5' NTR and P1 genes were sequenced and compared with sequences from GenBank database. Nucleotide sequence similarity ranged from 47–100% in the 5' NTR and from 63–100% in the P1 coding region. The lowest amino acid similarity between sequences of P1 gene was 55%. In phylogenetic trees of 5'NTR analysis, two PVY<sup>O </sup>Mexican isolates clustered with other PVY<sup>O </sup>isolates. In turn, the three PVY<sup>N </sup>isolates grouped with PVY<sup>N-NTN </sup>isolates. The phylogenetic analysis of P1 sequences (nucleotide and amino acid) showed two PVY<sup>O </sup>isolates grouping next to N-NTN cluster. A detailed analysis of the PVY<sup>O </sup>isolates showed two potential recombination regions in the P1 gene, in contrast to 5'NTR where no recombination was detected.</p> |
| first_indexed | 2024-12-11T03:36:32Z |
| format | Article |
| id | doaj.art-479a187a4bd34544894cf8b292613fe2 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-11T03:36:32Z |
| publishDate | 2009-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-479a187a4bd34544894cf8b292613fe22022-12-22T01:22:14ZengBMCVirology Journal1743-422X2009-05-01614810.1186/1743-422X-6-48Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoesMartínez-Soriano JuanSilva-Rosales LauraFrías-Treviño GustavoAviña-Padilla KatiaRamírez-Rodríguez Victoriano<p>Abstract</p> <p>As part of a routine screening for the possible presence of the necrotic strains of potato virus Y affecting potatoes in Mexico, five PVY isolates were submitted to biological and molecular analysis. Considering their serological properties, two belong to the common strain (O) and three to the necrotic strain (N). All the isolates induced vein necrosis in <it>Nicotiana tabacum</it>. To characterize the isolates, 5' NTR and P1 genes were sequenced and compared with sequences from GenBank database. Nucleotide sequence similarity ranged from 47–100% in the 5' NTR and from 63–100% in the P1 coding region. The lowest amino acid similarity between sequences of P1 gene was 55%. In phylogenetic trees of 5'NTR analysis, two PVY<sup>O </sup>Mexican isolates clustered with other PVY<sup>O </sup>isolates. In turn, the three PVY<sup>N </sup>isolates grouped with PVY<sup>N-NTN </sup>isolates. The phylogenetic analysis of P1 sequences (nucleotide and amino acid) showed two PVY<sup>O </sup>isolates grouping next to N-NTN cluster. A detailed analysis of the PVY<sup>O </sup>isolates showed two potential recombination regions in the P1 gene, in contrast to 5'NTR where no recombination was detected.</p>http://www.virologyj.com/content/6/1/48 |
| spellingShingle | Martínez-Soriano Juan Silva-Rosales Laura Frías-Treviño Gustavo Aviña-Padilla Katia Ramírez-Rodríguez Victoriano Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoes Virology Journal |
| title | Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoes |
| title_full | Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoes |
| title_fullStr | Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoes |
| title_full_unstemmed | Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoes |
| title_short | Presence of necrotic strains of <it>Potato virus Y </it>in Mexican potatoes |
| title_sort | presence of necrotic strains of it potato virus y it in mexican potatoes |
| url | http://www.virologyj.com/content/6/1/48 |
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