A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition
Human embryonic stem cell line WA01 was genetically modified using zinc-finger nucleases and the PiggyBac/transponson system to introduce a fluorescence reporter for VE-cadherin (VEC; tdTomato) and CD43 (eGFP). Phenotypic and functional assays for pluripotency revealed the modified hES cell reporter...
Main Authors: | Ho Sun Jung, Gene Uenishi, Akhilesh Kumar, Mi Ae Park, Matt Raymond, Dustin Fink, Ethan McLeod, Igor Slukvin |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2016-09-01
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Series: | Stem Cell Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506116301192 |
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