Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer
Abstract Background Breast cancer (BC) patients tend to suffer from distant metastasis, especially bone metastasis. Methods All the analysis based on open-accessed data was performed in R software, dependent on multiple algorithms and packages. The RNA levels of specific genes were detected using qu...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-023-11588-6 |
| _version_ | 1797376904308719616 |
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| author | Kang Yao Zhu Xiaojun Zhao Tingxiao Liao Shiyao Ji Lichen Zhang Wei Li Yanlei Tian Jinlong Ding Xiaoyan Zhang Jun Bi Qing Lv Jun |
| author_facet | Kang Yao Zhu Xiaojun Zhao Tingxiao Liao Shiyao Ji Lichen Zhang Wei Li Yanlei Tian Jinlong Ding Xiaoyan Zhang Jun Bi Qing Lv Jun |
| author_sort | Kang Yao |
| collection | DOAJ |
| description | Abstract Background Breast cancer (BC) patients tend to suffer from distant metastasis, especially bone metastasis. Methods All the analysis based on open-accessed data was performed in R software, dependent on multiple algorithms and packages. The RNA levels of specific genes were detected using quantitative Real-time PCR as a method of detecting the RNA levels. To assess the ability of BC cells to proliferate, we utilized the CCK8 test, colony formation, and the 5-Ethynyl-20-deoxyuridine assay. BC cells were evaluated for invasion and migration by using Transwell assays and wound healing assays. Results In our study, we identified the molecules involved in BC bone metastasis based on the data from multiple BC cohorts. Then, we comprehensively investigated the effect pattern and underlying biological role of these molecules. We found that in the identified molecules, the EMP1, ACKR3, ITGA10, MMP13, COL11A1, and THY1 were significantly correlated with patient prognosis and mainly expressed in CAFs. Therefore, we explored the CAFs in the BC microenvironment. Results showed that CAFs could activate multiple carcinogenic pathways and most of these pathways play an important role in cancer metastasis. Meanwhile, we noticed the interaction between CAFs and malignant, endothelial, and M2 macrophage cells. Moreover, we found that CAFs could induce the remodeling of the BC microenvironment and promote the malignant behavior of BC cells. Then, we identified MMP13 for further analysis. It was found that MMP13 can enhance the malignant phenotype of BC cells. Meanwhile, biological enrichment and immune infiltration analysis were conducted to present the effect pattern of MMP13 in BC. Conclusions Our result can improve the understanding of researchers on the underlying mechanisms of BC bone metastasis. |
| first_indexed | 2024-03-08T19:46:15Z |
| format | Article |
| id | doaj.art-479ce5c5d54847018b93db7da805815c |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-08T19:46:15Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-479ce5c5d54847018b93db7da805815c2023-12-24T12:21:39ZengBMCBMC Cancer1471-24072023-12-0123111710.1186/s12885-023-11588-6Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancerKang Yao0Zhu Xiaojun1Zhao Tingxiao2Liao Shiyao3Ji Lichen4Zhang Wei5Li Yanlei6Tian Jinlong7Ding Xiaoyan8Zhang Jun9Bi Qing10Lv Jun11Cancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeDepartment of Musculoskeletal Oncology, Sun Yat-sen University Cancer CenterCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeCancer Center, Department of Orthopedics, Affliated People`s Hospital, Zhejiang Provincial People`s Hospital, Hangzhou Medical CollegeAbstract Background Breast cancer (BC) patients tend to suffer from distant metastasis, especially bone metastasis. Methods All the analysis based on open-accessed data was performed in R software, dependent on multiple algorithms and packages. The RNA levels of specific genes were detected using quantitative Real-time PCR as a method of detecting the RNA levels. To assess the ability of BC cells to proliferate, we utilized the CCK8 test, colony formation, and the 5-Ethynyl-20-deoxyuridine assay. BC cells were evaluated for invasion and migration by using Transwell assays and wound healing assays. Results In our study, we identified the molecules involved in BC bone metastasis based on the data from multiple BC cohorts. Then, we comprehensively investigated the effect pattern and underlying biological role of these molecules. We found that in the identified molecules, the EMP1, ACKR3, ITGA10, MMP13, COL11A1, and THY1 were significantly correlated with patient prognosis and mainly expressed in CAFs. Therefore, we explored the CAFs in the BC microenvironment. Results showed that CAFs could activate multiple carcinogenic pathways and most of these pathways play an important role in cancer metastasis. Meanwhile, we noticed the interaction between CAFs and malignant, endothelial, and M2 macrophage cells. Moreover, we found that CAFs could induce the remodeling of the BC microenvironment and promote the malignant behavior of BC cells. Then, we identified MMP13 for further analysis. It was found that MMP13 can enhance the malignant phenotype of BC cells. Meanwhile, biological enrichment and immune infiltration analysis were conducted to present the effect pattern of MMP13 in BC. Conclusions Our result can improve the understanding of researchers on the underlying mechanisms of BC bone metastasis.https://doi.org/10.1186/s12885-023-11588-6Breast cancerBone metastasisPrognosisCAFsMMP13 |
| spellingShingle | Kang Yao Zhu Xiaojun Zhao Tingxiao Liao Shiyao Ji Lichen Zhang Wei Li Yanlei Tian Jinlong Ding Xiaoyan Zhang Jun Bi Qing Lv Jun Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer BMC Cancer Breast cancer Bone metastasis Prognosis CAFs MMP13 |
| title | Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer |
| title_full | Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer |
| title_fullStr | Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer |
| title_full_unstemmed | Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer |
| title_short | Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer |
| title_sort | multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer |
| topic | Breast cancer Bone metastasis Prognosis CAFs MMP13 |
| url | https://doi.org/10.1186/s12885-023-11588-6 |
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