In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells
3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated...
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2020-07-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/14/3057 |
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| author | Shuaishuai Cao Jonghyeuk Han Neha Sharma Bilal Msallem Wonwoo Jeong Jeonghyun Son Christoph Kunz Hyun-Wook Kang Florian M. Thieringer |
| author_facet | Shuaishuai Cao Jonghyeuk Han Neha Sharma Bilal Msallem Wonwoo Jeong Jeonghyun Son Christoph Kunz Hyun-Wook Kang Florian M. Thieringer |
| author_sort | Shuaishuai Cao |
| collection | DOAJ |
| description | 3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated 3D poly (lactic-co-glycolic acid)/β-tricalcium phosphate (3D-PLGA/TCP) and 3D β-tricalcium phosphate (3D-TCP) scaffolds using two relatively distinct 3D printing (3DP) technologies. Conjunctively, we compared and investigated mechanical and biological responses on human dental pulp stem cells (hDPSCs). Physicochemical properties of the scaffolds, including pore structure, chemical elements, and compression modulus, were characterized. hDPSCs were cultured on scaffolds for subsequent investigations of biocompatibility and osteoconductivity. Our findings indicate that 3D printed PLGA/TCP and β-tricalcium phosphate (β-TCP) scaffolds possessed a highly interconnected and porous structure. 3D-TCP scaffolds exhibited better compressive strength than 3D-PLGA/TCP scaffolds, while the 3D-PLGA/TCP scaffolds revealed a flexible mechanical performance. The introduction of 3D structure and β-TCP components increased the adhesion and proliferation of hDPSCs and promoted osteogenic differentiation. In conclusion, 3D-PLGA/TCP and 3D-TCP scaffolds, with the incorporation of hDPSCs as a personalized restoration approach, has a prospective potential to repair minor and critical bone defects in oral and maxillofacial surgery, respectively. |
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| id | doaj.art-47a0915be0dc4486b403f89e77dc07d8 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T18:36:26Z |
| publishDate | 2020-07-01 |
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| series | Materials |
| spelling | doaj.art-47a0915be0dc4486b403f89e77dc07d82023-11-20T06:12:21ZengMDPI AGMaterials1996-19442020-07-011314305710.3390/ma13143057In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem CellsShuaishuai Cao0Jonghyeuk Han1Neha Sharma2Bilal Msallem3Wonwoo Jeong4Jeonghyun Son5Christoph Kunz6Hyun-Wook Kang7Florian M. Thieringer8Department of Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Spitalstrasse 21, 4031 Basel, SwitzerlandBiomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 50, UNIST-gil, Ulsan 44919, KoreaDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Spitalstrasse 21, 4031 Basel, SwitzerlandDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Spitalstrasse 21, 4031 Basel, SwitzerlandBiomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 50, UNIST-gil, Ulsan 44919, KoreaBiomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 50, UNIST-gil, Ulsan 44919, KoreaDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Spitalstrasse 21, 4031 Basel, SwitzerlandBiomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 50, UNIST-gil, Ulsan 44919, KoreaDepartment of Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Spitalstrasse 21, 4031 Basel, Switzerland3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated 3D poly (lactic-co-glycolic acid)/β-tricalcium phosphate (3D-PLGA/TCP) and 3D β-tricalcium phosphate (3D-TCP) scaffolds using two relatively distinct 3D printing (3DP) technologies. Conjunctively, we compared and investigated mechanical and biological responses on human dental pulp stem cells (hDPSCs). Physicochemical properties of the scaffolds, including pore structure, chemical elements, and compression modulus, were characterized. hDPSCs were cultured on scaffolds for subsequent investigations of biocompatibility and osteoconductivity. Our findings indicate that 3D printed PLGA/TCP and β-tricalcium phosphate (β-TCP) scaffolds possessed a highly interconnected and porous structure. 3D-TCP scaffolds exhibited better compressive strength than 3D-PLGA/TCP scaffolds, while the 3D-PLGA/TCP scaffolds revealed a flexible mechanical performance. The introduction of 3D structure and β-TCP components increased the adhesion and proliferation of hDPSCs and promoted osteogenic differentiation. In conclusion, 3D-PLGA/TCP and 3D-TCP scaffolds, with the incorporation of hDPSCs as a personalized restoration approach, has a prospective potential to repair minor and critical bone defects in oral and maxillofacial surgery, respectively.https://www.mdpi.com/1996-1944/13/14/30573D printingdental biomaterialspolymer printingceramic printinghuman dental pulp stem cellin vitro research |
| spellingShingle | Shuaishuai Cao Jonghyeuk Han Neha Sharma Bilal Msallem Wonwoo Jeong Jeonghyun Son Christoph Kunz Hyun-Wook Kang Florian M. Thieringer In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells Materials 3D printing dental biomaterials polymer printing ceramic printing human dental pulp stem cell in vitro research |
| title | In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells |
| title_full | In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells |
| title_fullStr | In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells |
| title_full_unstemmed | In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells |
| title_short | In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells |
| title_sort | in vitro mechanical and biological properties of 3d printed polymer composite and β tricalcium phosphate scaffold on human dental pulp stem cells |
| topic | 3D printing dental biomaterials polymer printing ceramic printing human dental pulp stem cell in vitro research |
| url | https://www.mdpi.com/1996-1944/13/14/3057 |
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