Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrix
Amyloid-β oligomer (AβO) is believed to cause neurotoxicity which is linked to Alzheimer's disease (AD). Therefore, the detection of AβO has been proposed as an effective method for the early diagnosis of AD. In this work, several three-dimensional layers using gold nanoparticles (AuNPs) embedd...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Materials Today Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590049822000467 |
| _version_ | 1811257864713404416 |
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| author | Chen Zhao Audrey Wang Xiaoying Tang Jieling Qin |
| author_facet | Chen Zhao Audrey Wang Xiaoying Tang Jieling Qin |
| author_sort | Chen Zhao |
| collection | DOAJ |
| description | Amyloid-β oligomer (AβO) is believed to cause neurotoxicity which is linked to Alzheimer's disease (AD). Therefore, the detection of AβO has been proposed as an effective method for the early diagnosis of AD. In this work, several three-dimensional layers using gold nanoparticles (AuNPs) embedded in different conducting polymer matrix, including poly (thiophene-3-acetic acid), poly (pyrrole-2-carboxylic acid), and poly (pyrrole-3-carboxylic acid), were harnessed as transducers for the large surface area and high electrical conductivity. The cellular prion protein (PrPC) was utilized as the biorecognition element for the specific detection of AβO. The prepared electrochemical sensors were fabricated and compared afterward, in terms of sensitivity and detection range for the highly sensitive detection of AβO. In comparison, the PrPC/AuNPs embedded in Ppy-3-COOH matrix (AuNPs-E-Ppy-3-COOH matrix) was demonstrated to have higher sensitivity and wider detection range (10−9 to 103 nM). Subsequently, through the ex vivo real sample analysis within cerebrospinal fluid and blood test, it is proved that PrPC/AuNPs-E-Ppy-3-COOH matrix can be used for the early diagnosis of AD. |
| first_indexed | 2024-04-12T18:04:13Z |
| format | Article |
| id | doaj.art-47a1e5ba251a4045bec9087575267359 |
| institution | Directory Open Access Journal |
| issn | 2590-0498 |
| language | English |
| last_indexed | 2024-04-12T18:04:13Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Advances |
| spelling | doaj.art-47a1e5ba251a4045bec90875752673592022-12-22T03:22:03ZengElsevierMaterials Today Advances2590-04982022-06-0114100250Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrixChen Zhao0Audrey Wang1Xiaoying Tang2Jieling Qin3School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaTongji University Cancer Center, Shanghai Tenth People's Hospital, Shanghai Frontiers Science Center of Nanocatalytic Medicine, School of Medicine, Tongji University, Shanghai 200092, ChinaSchool of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaTongji University Cancer Center, Shanghai Tenth People's Hospital, Shanghai Frontiers Science Center of Nanocatalytic Medicine, School of Medicine, Tongji University, Shanghai 200092, China; Corresponding author.Amyloid-β oligomer (AβO) is believed to cause neurotoxicity which is linked to Alzheimer's disease (AD). Therefore, the detection of AβO has been proposed as an effective method for the early diagnosis of AD. In this work, several three-dimensional layers using gold nanoparticles (AuNPs) embedded in different conducting polymer matrix, including poly (thiophene-3-acetic acid), poly (pyrrole-2-carboxylic acid), and poly (pyrrole-3-carboxylic acid), were harnessed as transducers for the large surface area and high electrical conductivity. The cellular prion protein (PrPC) was utilized as the biorecognition element for the specific detection of AβO. The prepared electrochemical sensors were fabricated and compared afterward, in terms of sensitivity and detection range for the highly sensitive detection of AβO. In comparison, the PrPC/AuNPs embedded in Ppy-3-COOH matrix (AuNPs-E-Ppy-3-COOH matrix) was demonstrated to have higher sensitivity and wider detection range (10−9 to 103 nM). Subsequently, through the ex vivo real sample analysis within cerebrospinal fluid and blood test, it is proved that PrPC/AuNPs-E-Ppy-3-COOH matrix can be used for the early diagnosis of AD.http://www.sciencedirect.com/science/article/pii/S25900498220004673D conductive polymerAuNPsAmyloid beta oligomerImpedance sensorAD |
| spellingShingle | Chen Zhao Audrey Wang Xiaoying Tang Jieling Qin Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrix Materials Today Advances 3D conductive polymer AuNPs Amyloid beta oligomer Impedance sensor AD |
| title | Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrix |
| title_full | Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrix |
| title_fullStr | Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrix |
| title_full_unstemmed | Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrix |
| title_short | Electrochemical sensitive detection of amyloid-β oligomer harnessing cellular prion protein on AuNPs embedded poly (pyrrole-3-carboxylic acid) matrix |
| title_sort | electrochemical sensitive detection of amyloid β oligomer harnessing cellular prion protein on aunps embedded poly pyrrole 3 carboxylic acid matrix |
| topic | 3D conductive polymer AuNPs Amyloid beta oligomer Impedance sensor AD |
| url | http://www.sciencedirect.com/science/article/pii/S2590049822000467 |
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