A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluation
Staplers have been widely used in the clinical treatment of gastrointestinal reconstruction. However, the current titanium (Ti) staple will remain in the human body permanently, resulting in some adverse effects. In this study, we developed a type of biodegradable staple for colonic anastomosis usin...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-04-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22004194 |
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| author | Yue Zhang Jian Cao Mengmeng Lu Yi Shao Kewei Jiang Xiaodong Yang Xiaoyu Xiong Shan Wang Chenglin Chu Feng Xue Yingjiang Ye Jing Bai |
| author_facet | Yue Zhang Jian Cao Mengmeng Lu Yi Shao Kewei Jiang Xiaodong Yang Xiaoyu Xiong Shan Wang Chenglin Chu Feng Xue Yingjiang Ye Jing Bai |
| author_sort | Yue Zhang |
| collection | DOAJ |
| description | Staplers have been widely used in the clinical treatment of gastrointestinal reconstruction. However, the current titanium (Ti) staple will remain in the human body permanently, resulting in some adverse effects. In this study, we developed a type of biodegradable staple for colonic anastomosis using 0.3 mm diameter magnesium (Mg) alloy wires. The wire surface was modified by micro-arc oxidation treatment (MAO) and then coated with poly-l-lactic acid (PLLA) to achieve a moderate degradation rate matching the tissue healing process. The results of tensile tests on isolated porcine colon tissue anastomosed by Mg and Ti staples showed that the anastomotic property of Mg staples was almost equal to that of Ti staples. The in vitro degradation tests indicated the dual-layer coating effectively enhanced the corrosion resistance and maintained the tensile force of the coated staple stable after 14-day immersion in the simulated colonic fluid (SCF). Furthermore, 24 beagle dogs were employed to conduct a comparison experiment using Mg-based and clinical Ti staples for 90-day implantation by ent-to-side anastomosis of the colon. The integrated structure of Mg-based staples was observed after 7 days and completely degraded after 90 days. All animals did not have anastomotic leakage and stenosis, and 12 dogs with Mg-based staples fully recovered after 90 days without differences in visceral ion levels and other side effects. The favorable performance makes this Mg-based anastomotic staple an ideal candidate for colon reconstruction. |
| first_indexed | 2024-04-10T20:56:33Z |
| format | Article |
| id | doaj.art-47a224ad552e4a4490c202a3172ecaf6 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:34:22Z |
| publishDate | 2023-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-47a224ad552e4a4490c202a3172ecaf62024-04-16T17:41:26ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-04-0122225238A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluationYue Zhang0Jian Cao1Mengmeng Lu2Yi Shao3Kewei Jiang4Xiaodong Yang5Xiaoyu Xiong6Shan Wang7Chenglin Chu8Feng Xue9Yingjiang Ye10Jing Bai11Jiangsu Key Laboratory for Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing, 211189, China; Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, ChinaDepartment of Gastroenterological Surgery, Peking University People's Hospital, Beijing, 100044, ChinaDepartment of Oral Implantology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, 210029, ChinaJiangsu Key Laboratory for Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing, 211189, China; Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, ChinaDepartment of Gastroenterological Surgery, Peking University People's Hospital, Beijing, 100044, ChinaDepartment of Gastroenterological Surgery, Peking University People's Hospital, Beijing, 100044, ChinaDepartment of Gastroenterological Surgery, Peking University People's Hospital, Beijing, 100044, ChinaDepartment of Gastroenterological Surgery, Peking University People's Hospital, Beijing, 100044, ChinaJiangsu Key Laboratory for Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing, 211189, ChinaJiangsu Key Laboratory for Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing, 211189, China; Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, ChinaDepartment of Gastroenterological Surgery, Peking University People's Hospital, Beijing, 100044, China; Corresponding author.Jiangsu Key Laboratory for Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing, 211189, China; Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, China; Jiangsu Key Laboratory for Light Metal Alloys, Nanjing, 211212, China; Corresponding author. Jiangsu Key Laboratory for Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing, 211189, China.Staplers have been widely used in the clinical treatment of gastrointestinal reconstruction. However, the current titanium (Ti) staple will remain in the human body permanently, resulting in some adverse effects. In this study, we developed a type of biodegradable staple for colonic anastomosis using 0.3 mm diameter magnesium (Mg) alloy wires. The wire surface was modified by micro-arc oxidation treatment (MAO) and then coated with poly-l-lactic acid (PLLA) to achieve a moderate degradation rate matching the tissue healing process. The results of tensile tests on isolated porcine colon tissue anastomosed by Mg and Ti staples showed that the anastomotic property of Mg staples was almost equal to that of Ti staples. The in vitro degradation tests indicated the dual-layer coating effectively enhanced the corrosion resistance and maintained the tensile force of the coated staple stable after 14-day immersion in the simulated colonic fluid (SCF). Furthermore, 24 beagle dogs were employed to conduct a comparison experiment using Mg-based and clinical Ti staples for 90-day implantation by ent-to-side anastomosis of the colon. The integrated structure of Mg-based staples was observed after 7 days and completely degraded after 90 days. All animals did not have anastomotic leakage and stenosis, and 12 dogs with Mg-based staples fully recovered after 90 days without differences in visceral ion levels and other side effects. The favorable performance makes this Mg-based anastomotic staple an ideal candidate for colon reconstruction.http://www.sciencedirect.com/science/article/pii/S2452199X22004194Gastrointestinal tractDegradationBursting pressureAnastomotic stomaColon reconstruction |
| spellingShingle | Yue Zhang Jian Cao Mengmeng Lu Yi Shao Kewei Jiang Xiaodong Yang Xiaoyu Xiong Shan Wang Chenglin Chu Feng Xue Yingjiang Ye Jing Bai A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluation Bioactive Materials Gastrointestinal tract Degradation Bursting pressure Anastomotic stoma Colon reconstruction |
| title | A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluation |
| title_full | A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluation |
| title_fullStr | A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluation |
| title_full_unstemmed | A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluation |
| title_short | A biodegradable magnesium surgical staple for colonic anastomosis: In vitro and in vivo evaluation |
| title_sort | biodegradable magnesium surgical staple for colonic anastomosis in vitro and in vivo evaluation |
| topic | Gastrointestinal tract Degradation Bursting pressure Anastomotic stoma Colon reconstruction |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22004194 |
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