Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles

Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles

Abstract Small extracellular vesicles (sEVs) provide major promise for advances in cancer diagnostics, prognostics, and therapeutics, ascribed to their distinctive cargo reflective of pathophysiological status, active involvement in intercellular communication, as well as their ubiquity and stabilit...

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Bibliographic Details
Main Authors: Kekoolani S. Visan, Richard J. Lobb, Sunyoung Ham, Luize G. Lima, Carlos Palma, Chai Pei Zhi Edna, Li‐Ying Wu, Harsha Gowda, Keshava K. Datta, Gunter Hartel, Carlos Salomon, Andreas Möller
Format: Article
Language:English
Published: Wiley 2022-09-01
Series:Journal of Extracellular Vesicles
Subjects:
cell culture
extracellular vesicles
isolation
tangential flow filtration
Online Access:https://doi.org/10.1002/jev2.12266

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